General Gibberellic Acid Elisa Kit

  • Product name: Gibberellic acid (GA), ELISA kit
  • Full Product Name: General Gibberellic Acid (GA) ELISA Kit
  • Product synonym names: GA3; Gibberellin A3; Gibberellins
  • Product gene name: GA ELISA kit
  • Species reactivity: General

Specificity

  • This assay has high sensitivity and excellent specificity for the detection of gibberellic acid (GA).
  • No significant cross-reactivity or interference was observed between gibberellic acid (GA) and its analogs.

Test type: Competitive inhibition

Samples: Cell or tissue culture supernatants

Detection range: 123.5-10,000 ng / ml

Sensitivity: <52.1 ng / ml

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Enzyme-linked immunosorbent assay for antigen detection.

Intra-assay precision (precision within an assay)

3 samples were tested with low, medium, and high-level gibberellic acid (GA) 20 times on one plate, respectively.

Inter-assay precision (precision between assays)

3 samples were tested with low, medium, and high-level gibberellic acid (GA) on 3 different plates, with 8 replicates on each plate.

CV (%) =

SD / media X100

Intra-assay: CV <10%

Intense: CV <12%

Preparation and storage

  • The stability of the kit is determined by the rate of loss of activity. The loss rate for this kit is less than 5% within the expiration date under proper storage conditions.
  • To minimize the further influence on performance, operating procedures and laboratory conditions, especially ambient temperature, air humidity, and incubator temperature, must be strictly controlled. It is also strongly suggested that the entire test be performed by the same operator from start to finish.

ISO certification

Manufactured in an ISO 9001: 2008 and ISO 13485: 2003 certified laboratory.

Supply chain verification

Lab-made with traceable raw materials. Generally, bulk orders can be prepared to customer specifications, please inquire.

Product note

Information selected from the online data sheet is extracted from bioinformatics databases, occasionally resulting in the ambiguous or irrelevant product information. It is the customer’s responsibility to review, verify and evaluate the information to make sure it matches their requirements before purchasing the kit. The essays in our ELISA kit are dynamic research tools and can sometimes be updated and improved.

If the format of this assay is important to you, please request the current manual or contact our technical support team with a pre-sale consultation before placing an order. We will confirm the current details of the trial. We cannot guarantee that the sample manual posted online is the most current manual, it is intended to serve as an example only. See the instructions for use provided with the test kit for precise details.

Other notes

Occasionally, small volumes of GA ELISA kit vials may become trapped in the product vial seal during shipping and storage. If necessary, briefly centrifuge the vial in a benchtop centrifuge to dislodge any liquid on the lid of the container. Some products may need to be shipped with dry ice and an additional fee may apply for dry ice.

Search terms for GA purchases

MBS2700284 is a ready-to-use microwell plate ELISA (enzyme-linked immunosorbent assay) kit for testing the presence of gibberellic acid (GA) ELISA kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls provide a theoretical detection range of the kit in biological research samples containing GA.

The analytical biochemical ELISA technique of the MBS2700284 kit is based on GA antibody-GA antigen interactions (immunosorbent) and an HRP colorimetric detection system to detect GA antigen targets in samples. The ELISA Kit is designed to detect native, non-recombinant GA. Appropriate sample types may include secretions and undiluted body fluids and/or tissue homogenates. Quality control trials evaluating reproducibility identified the intra-assay CV (%) and inter-assay CV (%).

Related Product Information for GA ELISA kit

This assay employs the competitive inhibition enzyme immunoassay technique. A microplate has been pre-coated with a gibberellic acid (GA) specific monoclonal antibody. A competitive inhibition reaction between biotin-labeled gibberellic acid (GA) and unlabeled gibberellic acid (GA) (standards or samples) is launched with the gibberellic acid (GA) -specific pre-coated antibody.

After incubation, the unbound conjugate is washed off. Horseradish peroxidase (HRP) conjugated avidin is then added to each well of the microplate and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of gibberellic acid (GA) in the sample. After the addition of the substrate solution, the intensity of the color developed is inversely proportional to the concentration of gibberellic acid (GA) in the sample.

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