Detection of SARS-CoV-2 using qRT-PCR

Detection of SARS-CoV-2 utilizing qRT-PCR in saliva obtained from asymptomatic or gentle COVID-19 sufferers, comparative evaluation with matched nasopharyngeal samples

Targets: The correct detection of extreme acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) is important for the prognosis of coronavirus illness 2019 (COVID-19). We in contrast the quantitative RT-PCR outcomes between nasopharyngeal swabs and saliva specimens.
Strategies: A COVID-19 outbreak occurred on a cruise ship at Nagasaki port, Japan. We obtained 123 nasopharyngeal swabs and saliva every from asymptomatic or gentle sufferers within the late section of an infection.
Outcomes: The intervals from the prognosis to the sampling had been 25.5 days for nasopharyngeal swabs and 28.9 days for saliva. The optimistic fee was 19.5% (24/123) for nasopharyngeal swabs and 38.2% (47/123) for saliva (P = 0.48). The quantified viral copies (imply ± SEM copies/5 μl) had been 9.3±2.6 in nasopharyngeal swabs and 920±850 in saliva (P = 0.0006).
Conclusions: Some great benefits of saliva specimens embrace optimistic fee enchancment and correct viral load detection. Saliva could also be used as a dependable pattern for SARS-CoV-2 detection.
russelldurrett
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Bovine Colon Genomic DNA

BG-311 0.1mg
EUR 177

Bovine Colon Total Protein

BT-311 1mg
EUR 153

Bovine Colon Tissue Lysate

IBOCLNTL100UG each
EUR 541
Description: Bovine Colon Tissue Lysate

Bovine Colon Frozen Sections

BF-311 10 slides
EUR 261

Bovine Colon Paraffin Sections

BP-311 10 slides
EUR 240

Bovine Colon Cancer Antigen ELISA kit

E01A80880 96T
EUR 700
Description: ELISA

Dog Colon cDNA

DD-311 30 Reactions
EUR 280

Cat Colon cDNA

FD-311 30 Reactions
EUR 280

Pig Colon cDNA

PD-311 30 reactions
EUR 243

Rat Colon cDNA

RD-311 30 reactions
EUR 243

Sheep Colon cDNA

SD-311 30 reactions
EUR 243

Equine Colon cDNA

ED-311 30 reactions
EUR 319

Rabbit Colon cDNA

TD-311 30 reactions
EUR 243

Bovine Colon Cancer Antigen(CCA) ELISA Kit

NSL0064Bo 96 T
EUR 528

Hamster Colon cDNA

AD-311 30 reactions
EUR 243

MiniPig Colon cDNA

ND-311 30 reactions
EUR 358

cDNA - Lupus: Colon

C1236090Lup 40 reactions
EUR 811

Mouse CD1 Colon cDNA

MD-311 30 reactions
EUR 243

Mouse BLC Colon cDNA

MD-311-BLC 30 reactions
EUR 280

Mouse C57 Colon cDNA

MD-311-C57 30 reactions
EUR 280

Bovine Colon Cancer specific antigene 2 ELISA kit

E01A80884 96T
EUR 700
Description: ELISA

Bovine Colon Cancer specific antigene 3 ELISA kit

E01A80885 96T
EUR 700
Description: ELISA

Bovine Colon Cancer specific antigene 4 ELISA kit

E01A80886 96T
EUR 700
Description: ELISA

Guinea Pig Colon cDNA

GD-311 30 reactions
EUR 243

Monkey Colon cDNA, Rhesus

UD-311 30 reactions
EUR 316

Monkey Colon cDNA, Cynomolgus

KD-311 30 reactions
EUR 316

Human Colon cDNA-Oligo-dT

HD-311 30 reactions
EUR 280

Rat Colon cDNA-Random Primer

RD-311-RH 30 reactions
EUR 243

cDNA - Liver Cirrhosis: Colon

C1236090Lcs 40 reactions
EUR 802

Human Colon cDNA-Random Primer

HD-311-HR 30 reactions
EUR 280

Rat WS Colon cDNA-Oligo-dT

RD-311-WS 30 reactions
EUR 243

Bovine Skin cDNA

BD-101 30 reactions
EUR 243

Bovine Lung cDNA

BD-601 30 reactions
EUR 243

Bovine Liver cDNA

BD-314 30 reactions
EUR 243

Bovine Ovary cDNA

BD-406 30 reactions
EUR 243

Bovine Heart cDNA

BD-801 30 reactions
EUR 243

Bovine Aorta cDNA*

BD-807 30 reactions
EUR 280

Bovine Testis cDNA

BD-401 30 reactions
EUR 243

Bovine Uterus cDNA

BD-411 30 reactions
EUR 243

Bovine Pineal cDNA*

BD-505 30 reactions
EUR 280

Bovine Spleen cDNA

BD-701 30 reactions
EUR 243

Bovine Thymus cDNA

BD-702 30 reactions
EUR 243

Bovine Kidney cDNA

BD-901 30 reactions
EUR 243

Bovine Stomach cDNA

BD-302 30 reactions
EUR 243

Bovine Thyroid cDNA*

BD-503 30 reactions
EUR 280

Bovine Bladder cDNA*

BD-902 30 reactions
EUR 243

cDNA - Rat Normal Tissue: Colon

C1434090 40 reactions
EUR 636

Bovine Prostate cDNA*

BD-408 30 reactions
EUR 243

Bovine Placenta cDNA

BD-413 30 reactions
EUR 243

cDNA - Human Tumor Tissue: Colon

C1235090 40 reactions
EUR 636

Mouse C57 Colon cDNA-Random Primer

MD-311-C57-RH 30 reactions
EUR 280

Mouse CD1 Colon cDNA-Random Primer

MD-311-HR 30 reactions
EUR 243

Bovine Esophagus cDNA

BD-301 30 reactions
EUR 243

Bovine Pituitary cDNA*

BD-502 30 reactions
EUR 243

cDNA - Mouse Normal Tissue: Colon

C1334090 40 reactions
EUR 636

Bovine Epididymis cDNA

BD-402 30 reactions
EUR 243

Monkey Colon cDNA-Random Primer, Rhesus

UD-311-RH 30 reactions
EUR 316

Monkey Colon cDNA-Random Primer, Cynomolgus

KD-311-RH 30 reactions
EUR 316

Bovine Eye, Lens cDNA*

BD-117 30 reactions
EUR 280

Bovine Eye, Whole cDNA

BD-106 30 reactions
EUR 243

Bovine Eye, Cornea cDNA*

BD-116 30 reactions
EUR 280

Bovine Eye, Retina cDNA*

BD-118 30 reactions
EUR 280

Bovine Brain Stem cDNA*

BD-215 30 reactions
EUR 280

Bovine Spinal Cord cDNA*

BD-230 30 reactions
EUR 280

Bovine Lymph Nodes cDNA

BD-703 30 reactions
EUR 280

Bovine Bone Marrow cDNA*

BD-704 30 reactions
EUR 243

Bovine Corpus Luteum cDNA*

BD-417 30 reactions
EUR 280

Bovine Adrenal, whole cDNA*

BD-501 30 reactions
EUR 243

Bovine Adipose Tissue cDNA*

BD-103 30 reactions
EUR 280

Bovine Brain, Striatum cDNA*

BD-214 30 reactions
EUR 280

Bovine Fallopian Tube cDNA*

BD-410 30 reactions
EUR 280

Bovine Adrenal, Cortex cDNA*

BD-506 30 reactions
EUR 280

cDNA - Human Adult Normal Tissue: Colon

C1234090 40 reactions
EUR 424

Bovine Adrenal, Medulla cDNA*

BD-507 30 reactions
EUR 280

Bovine Skeletal Muscles cDNA

BD-102 30 reactions
EUR 243

Bovine Brain, Cerebellum cDNA

BD-202 30 reactions
EUR 243

Bovine Salivary, Parotid cDNA

BD-316 30 reactions
EUR 243

cDNA - Monkey (Rhesus) Normal Tissue: Colon

C1534090 40 reactions
EUR 481

Bovine Brain, Hippocampus cDNA*

BD-203 30 reactions
EUR 280

Bovine Brain, Hypothalamus cDNA*

BD-204 30 reactions
EUR 280

cDNA - Human Diabetic Diseased Tissue: Colon

C1236090Dia 40 reactions
EUR 802

cDNA - Monkey (Cynomolgus) Normal Tissue: Colon

C1534090-Cy 40 reactions
EUR 481

Bovine Salivary, Submaxillary cDNA

BD-317 30 reactions
EUR 243

Bovine Dorsal Root Ganglia cDNA

BD-240 30 reactions
EUR 243

Bovine Brain, Cerebral Cortex cDNA

BD-210 30 reactions
EUR 243

Bovine Brain, Caudate Nucleus cDNA*

BD-242 30 reactions
EUR 280

Bovine Brain, Choroids Plexus cDNA

BD-243 30 reactions
EUR 280

Bovine Small Intestine, Ileum cDNA

BD-309 30 reactions
EUR 243

cDNA - Human Adult Normal Tissue: Colon Sigmoid

C1234095 40 reactions
EUR 424

Bovine Small Intestine, Jejunum cDNA

BD-308 30 reactions
EUR 243

Bovine Small Intestine, Duodenum cDNA

BD-307 30 reactions
EUR 243

cDNA - Monkey (Rhesus) Normal Tissue: Colon Sigmoid

C1534095 40 reactions
EUR 723

cDNA - Human Adult Normal Tissue: Colon Ascending

C1234091 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Colon Descending

C1234092 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Colon Transverse

C1234096 40 reactions
EUR 424

cDNA - Monkey (Rhesus) Normal Tissue: Colon Ascending

C1534091 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Colon descending

C1534092 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Colon Transverse

C1534096 40 reactions
EUR 723

Bovine Universal Reference cDNA-Oligo dT

BD-UR-40 1x40 reactions
EUR 243

Bovine Universal Reference cDNA-Oligo dT

BD-UR-80 2x40 reactions
EUR 461

Bovine Tissue cDNA Panel, any 10 Tissues

BD-010 10x10 reactions
EUR 1294

Bovine Universal Reference cDNA-Random Hexamer

BD-UR-40-RH 1x40 reactions
EUR 243

Bovine Universal Reference cDNA-Random Hexamer

BD-UR-80-RH 2x40 reactions
EUR 461

Genorise® Bovine IL-1b cDNA Clone, 10 ug

GR152001 10 µg
EUR 153

Bovine Colony Stimulating Factor 1 ELISA kit

E01A80684 96T
EUR 700
Description: ELISA

Bovine colony-stimulating factor(CSF) ELISA Kit

NSL1488Bo 96 T
EUR 528

Matched Pair - cDNA - Human Primary Tumor and Normal Tissue: Colon

C8235090-PP 10 reactions x2
EUR 583

Bovine Colony Stimulating Factor 2 Receptor Alpha ELISA kit

E01A80809 96T
EUR 700
Description: ELISA

Matched Pair - cDNA - Human Primary and Matched Metastatic Tumor Tissue: Colon

C8235090-PM 10 reactions x2
EUR 1212

Bovine Eosinophil colony stimulating factor ELISA kit

E01A82789 96T
EUR 700
Description: ELISA

Bovine Granulocyte Colony Stimulating Factor ELISA kit

E01A83233 96T
EUR 700
Description: ELISA

Bovine Anti Granulocyte Colony Stimulating Factor Antibody

E01A80193 96T
EUR 700
Description: ELISA

Bovine Granulocyte colony-stimulating factor(CSF3) Elisa kit

EK760046 96 Wells
EUR 0.9

Bovine CSF3/Granulocyte colony-stimulating factor ELISA Kit

EK19191 96Т
EUR 650

Bovine Granulocyte colony-stimulating factor(CSF3) ELISA kit

CSB-EL006049BO-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Bovine Granulocyte colony-stimulating factor (CSF3) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Bovine Granulocyte colony-stimulating factor(CSF3) ELISA kit

1-CSB-EL006049BO
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  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Bovine Granulocyte colony-stimulating factor(CSF3) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Bovine Granulocyte colony-stimulating factor (CSF3) ELISA Kit

RK06106 96T
EUR 280

Bovine CSF3 / Granulocyte colony-stimulating factor ELISA Kit

E0042b 96T
EUR 900

Bovine Granulocyte Colony Stimulating Factor(G-CSF) ELISA Kit

NSL0295Bo 96 T
EUR 528

Bovine Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit

QY-E60021 96T
EUR 511.2

Bovine Macrophage colony stimulating factor receptor ELISA kit

E01A84251 96T
EUR 700
Description: ELISA

Bovine Granulocyte macrophage colony stimulating factor, GM- CSF

ELA-E0045b 96 Tests
EUR 1113.6

Bovine Macrophage Colony-Stimulating Factor(M-CSF)ELISA Kit

NSL1682Bo 96 T
EUR 528

Bovine Granulocyte Colony Stimulating Factor Receptor ELISA kit

E01A83234 96T
EUR 700
Description: ELISA

Bovine Colostrum Powder, 20% Igg

BCP-0100 1kg
EUR 298

Bovine Granulocyte Macrophage Colony Stimulating Factor ELISA kit

E01A83183 96T
EUR 700
Description: ELISA

Bovine Granulocyte colony-stimulating Factor, CSF3 GENLISA ELISA

KLB2212 1 x 96 wells
EUR 341

Colon

TMA2081 each
EUR 546
Description: Colon, prostate and breast tissue array, 156 cases/208 cores

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine)

4-PAA042Bo01
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  • 100ul
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  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF)

Bovine GM-CSF(Granulocyte Macrophage Colony Stimulating Factor) ELISA Kit

EB0161 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Cattle;Sensitivity: 9.375pg/ml

Bovine Macrophage Colony-Stimulating Factor Receptor(M-CSFR) ELISA Kit

NSL0577Bo 96 T
EUR 528

Bovine CSF2/ Granulocyte-macrophage colony-stimulating factor ELISA Kit

E0071Bo 1 Kit
EUR 630
Description: Colony-stimulating factor,CSF

Bovine Granulocyte-macrophage colony-stimulating factor(CSF2) ELISA kit

E01A83364 96T
EUR 700
Description: ELISA

Bovine Granulocyte-macrophage colony-stimulating factor(CSF2) Elisa kit

EK760043 96 Wells
EUR 0.9

Bovine Granulocyte-macrophage colony-stimulating factor(CSF2) ELISA kit

CSB-EL006045BO-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Bovine Granulocyte-macrophage colony-stimulating factor (CSF2) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Bovine Granulocyte-macrophage colony-stimulating factor(CSF2) ELISA kit

1-CSB-EL006045BO
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  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Bovine Granulocyte-macrophage colony-stimulating factor(CSF2) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Bovine Granulocyte-macrophage colony-stimulating factor (CSF2) ELISA Kit

RK06111 96T
EUR 280

Bovine CSF2 / Granulocyte-macrophage colony-stimulating factor ELISA Kit

E0045b 96T
EUR 900

Colon-26

CSC-C8788H One Frozen vial Ask for price
Description: Colon Adenocarcinoma Cells

Bovine Granulocyte-Macrophage Colony Stimulating Factor(GM-CSF) ELISA Kit

NSL0320Bo 96 T
EUR 528

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), APC

4-PAA042Bo01-APC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
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Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with APC.

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), Cy3

4-PAA042Bo01-Cy3
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  • 100ul
  • 10ml
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  • 200ul
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Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with Cy3.

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), HRP

4-PAA042Bo01-HRP
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
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Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with HRP.

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), PE

4-PAA042Bo01-PE
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
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Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with PE.

Recombinant Bovine Granulocyte-macrophage colony-stimulating factor (CSF2)

CSB-YP006045BO 7329 mg Ask for price

Ict1 (GFP-tagged) - Mouse immature colon carcinoma transcript 1 (cDNA clone MGC:41300 IMAGE:2938122)

MG202185 10 µg Ask for price

Colon cancer with colon tissue array

CO1021a each
EUR 306
Description: Colon cancer with colon tissue array, including pathology grade, with MMR (MLH1/PMS2/MSH2/MSH6) IHC results,TNM and clinical stage, containing 86 cases of adenocarcinoma, 4 papillary adenocarcinoma, 7 mucinous adenocarcinoma, 2 adenoma, 1 congenital megacolon, 2 colon tissue, 102 cases/102 cores replacing CO1021

Colon cancer with colon tissue array

CO1921 each
EUR 546
Description: Colon cancer with colon tissue array, including pathology grade, TNM and clinical stage, 192 cases/192 cores

Colon cancer with colon tissue array

CO2161b each
EUR 474
Description: Colon cancer with colon tissue array, including pathology grade, TNM and clinical stage, 216 cases/216 cores, replacing CO2161a

Colon cancer with colon tissue array

CO721 each
EUR 270
Description: Colon cancer with colon tissue array, including pathology grade, TNM and clinical stage, 24 cases/72 cores

Colon cancer with colon tissue array

CO805 each
EUR 258
Description: Colon cancer with colon tissue array, including pathology grade, TNM and clinical stage, 40 cases/80 cores

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), FITC

4-PAA042Bo01-FITC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
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Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with FITC.

Ict1 (untagged) - Mouse immature colon carcinoma transcript 1 (cDNA clone MGC:41300 IMAGE:2938122), (10ug)

MC206924 10 µg Ask for price

Innovative Grade US Origin Bovine Colostrum

IGBOBMKCOF1000ML each
EUR 210
Description: Innovative Grade US Origin Bovine Colostrum

Innovative Grade US Origin Bovine Colostrum

IGBOBMKCOZ1000ML each
EUR 210
Description: Innovative Grade US Origin Bovine Colostrum

Bovine Granulocyte-macrophage colony-stimulating Factor, CSF2 GENLISA ELISA

KLB2213 1 x 96 wells
EUR 341

Bovine Anti Granulocyte macrophage colony stimulating factor antibody ELISA kit

E01A83343 96T
EUR 700
Description: ELISA

Bovine Granulocyte-Macrophage Colony Stimulating Factor, GM-CSF GENLISA ELISA

KLB0430 1 x 96 wells
EUR 341

Colon adenocarcinoma with colon tissue array

CO483b each
EUR 198
Description: Colon adenocarcinoma with colon tissue array, including pathology, TNM and clinical stage, 48 cases/48 cores, replacing CO483a

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), APC-Cy7

4-PAA042Bo01-APC-Cy7
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
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Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with APC-Cy7.

Colon tissue array with colon cancer tissue

BN05014a each
EUR 222
Description: Colon tissue array with colon cancer tissue, including pathology grade, TNM and clinical stage, 24 cases/72 cores, replacing BN05014

Ict1 (Myc-DDK-tagged) - Mouse immature colon carcinoma transcript 1 (cDNA clone MGC:41300 IMAGE:2938122)

MR202185 10 µg Ask for price

Colon cancer with matched colon tissue array

CO1801a each
EUR 474
Description: Colon cancer with matched colon tissue array, including pathology grade,with MMR (MLH1/PMS2/MSH2/MSH6) IHC results, TNM and clinical stage, containing 90 cases of adenocarcinoma, and 90 cancer adjacent or adjacent normal colon tissue, 90 cases/180 cores, replacing CO1801

Colon cancer with matched colon tissue array

CO602a each
EUR 222
Description: Colon cancer with matched colon tissue array, including pathology grade, TNM and clinical stage, 30 cases/60 cores, replacing CO602

Colon cancer with matched colon tissue array

CO703 each
EUR 198
Description: Colon cancer with matched colon tissue array, including pathology grade, TNM and clinical stage, 35 cases/70 cores,replaced by CO703a

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), Biotinylated

4-PAA042Bo01-Biotin
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  • 100ul
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Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with Biotin.

Human Colon

HS0024 6 Slides/Pack
EUR 160

Stard13 (GFP-tagged) - Mouse serologically defined colon cancer antigen 13 (cDNA clone MGC:38133 IMAGE:5320966)

MG211650 10 µg Ask for price

Sdccag8 (untagged) - Mouse serologically defined colon cancer antigen 8 (cDNA clone MGC:28854 IMAGE:4507575), (10ug)

MC218488 10 µg Ask for price

Sdccag10 (GFP-tagged) - Mouse serologically defined colon cancer antigen 10 (cDNA clone MGC:29160 IMAGE:5025084)

MG205684 10 µg Ask for price

Sdccag3 (GFP-tagged) - Mouse serologically defined colon cancer antigen 3 (cDNA clone MGC:37699 IMAGE:5064948)

MG205506 10 µg Ask for price

Sdccag8 (GFP-tagged) - Mouse serologically defined colon cancer antigen 8 (cDNA clone MGC:28854 IMAGE:4507575)

MG205648 10 µg Ask for price

Colon Lysate

1472 0.1 mg
EUR 229.2
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Colon Lysate

21-179 0.1 mg
EUR 342.6
Description: Monkey (Cynomolgus) colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) colon tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Colon Lysate

21-288 0.1 mg
EUR 342.6
Description: Monkey (Rhesus) colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Rhesus) colon tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Colon adenocarcinoma tissue array with colon tissue

CO1201 each
EUR 348
Description: Colon adenocarcinoma tissue array with colon tissue, with pathology grade, TNM/Stage, 120 cases/120 cores (core size 1.0mm), replacing BC051110c

Comparative Analysis of Two Automated Multiplex RT-PCR Assessments for Fast Detection of Influenza and Respiratory Syncytial Viruses

Background: Fast and correct prognosis of influenza virus (Flu) and respiratory syncytial virus (RSV) is vital for managing each the affected person and laboratory. We in contrast the cobas Influenza A/B & RSV assay (cobas Liat) with the Simplexa Flu A/B & RSV assay (Simplexa) to guage which check methodology is extra advantageous contemplating the sources of the laboratory and outcomes of check efficiency.
Strategies: A complete of 236 respiratory specimens from sufferers referred for respiratory virus testing had been retrospectively evaluated; 53 specimens examined optimistic for every of Flu A, Flu B, and RSV, and 77 specimens examined unfavorable based mostly on the outcomes of the reference methodology, i.e., the Seegene Allplex Respiratory Panel half/3 (Seegene, Seoul, Korea). The turnaround time (TAT) was 20 minutes per specimen for cobas Liat and 78 minutes per eight speci-mens for Simplexa. The overall hands-on time was round one minute per specimen for each assessments. The specimen quantity required for testing was 200 µL for cobas Liat and 50 µL for Simplexa. Seegene Allplex Respiratory Panel half/Three was used because the reference methodology.
Outcomes: The variety of invalid outcomes was 1 (0.4%) for cobas Liat and 10 (4.2%) for Simplexa (p < 0.05). All outcomes had been according to these of the reference methodology in cobas Liat. The sensitivity and specificity for Flu A, Flu B, and RSVA had been 100% with Simplexa. Nevertheless, the sensitivity for RSVB was 80.0% with Simplexa, which was a statistically vital distinction with the discovering for cobas Liat (p < 0.05). Comparability of the cycle threshold (Ct) values of RSV for Simplexa with the reference methodology confirmed correlation as steady variables (p < 0.001) with a better propensity for acquiring Ct values with Simplexa, the exception being the six false unfavorable outcomes; their Ct values had been greater than 30 within the reference methodology.
Conclusions: Cobas Liat confirmed correct efficiency with a fast TAT and an excellent workflow effectivity. Cobas Liat is extra environment friendly than Simplexa as a point-of-care check for the detection of RSV.

Disagreement between PCR and serological prognosis of Trypanosoma cruzi an infection in blood donors from a Colombian endemic area

Introduction: Chagas’ illness is the main reason behind infectious myocarditis worldwide. This an infection brought on by Trypanosoma cruzi is often life-long and asymptomatic; nonetheless, the third a part of contaminated folks can develop extreme and even deadly cardiomyopathy. Because the parasitemia within the persistent section is each low-grade and intermittent, T. cruzi an infection is principally detected by serology, though this methodology has sensitivity and specificity limitations.
Goal: To find out the extent of settlement between serologic and molecular assessments in 658 voluntary blood donors from six provinces within the Colombian division of Santander.
Supplies and strategies: We evaluated an array of diagnostic applied sciences by cross-section sampling performing a serological double diagnostic check for T. cruzi antibody detection (Chagas III ELISA™, BiosChile Group, and ARCHITECT Chagas CMIA™, Abbott), and DNA detection by polymerase chain response (PCR). We collected the demographic, medical, and epidemiological data of contributors. The pattern measurement was calculated utilizing Epidat™ and the statistical evaluation was finished with Stata 12.1™.
Outcomes: PCR was six occasions extra delicate in detecting T. cruzi an infection than ELISA/CMIA with prevalence values of 1.8% (12/658) and 0.3% (2/658), respectively, and kappa=0.28 (95%CI: -0.03 – 0.59). In distinction, serology confirmed a sensitivity of 16.7% (95%CI: 2.09 – 48.4) and a specificity of 100% (95%CI: 99.4 – 100). All seropositive samples had been discovered to be optimistic by PCR.
Conclusions: The implementation of PCR as a complementary methodology for screening donors might scale back the likelihood of false unfavorable and the resultant danger of transfusional-transmission of Chagas’ illness, particularly in endemic areas.

Simultaneous screening of the FRAXA and FRAXE loci for fast detection of FMR1 CGG and/or AFF2 CCG repeat expansions by triplet-primed PCR

Reasonable to hyper growth of trinucleotide repeats on the FRAXA and FRAXE fragile websites, with or with out concomitant hypermethylation are related to mental incapacity and different situations. Not like molecular prognosis of FMR1 CGG repeat expansions in FRAXA, present detection of AFF2 CCG repeat expansions in FRAXE depends on low-throughput and inefficient strategies combining Southern blot and PCR. A novel triplet-primed PCR assay was developed to concurrently display for trinucleotide repeat expansions on the FRAXA and FRAXE fragile websites, and validated utilizing archived medical samples of identified FMR1 and AFF2 genotypes.
Sequencing of inhabitants samples and FRAXE-affected samples was carried out to guage variation of the AFF2 CCG repeat construction. The duplex assay precisely recognized expansions on the FMR1 and AFF2 trinucleotide repeat loci. Sanger sequencing of the AFF2 CCG repeat revealed that the SNP variant rs868914124(C), which successfully provides two CCG repeats at the 5′ finish, is enriched within the Malay inhabitants and with brief repeats (<11 CCGs), and was current in all six expanded AFF2 alleles of this research. All expanded AFF2 alleles contained a number of non-CCG interruptions in the direction of the 5′ finish of the repeat. We’ve developed a delicate, sturdy and fast assay to concurrently detect growth mutations on the FMR1 and AFF2 trinucleotide repeat loci, simplifying the screening of FRAXA and FRAXE related issues.

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