Detection of SARS-CoV-2 using qRT-PCR

Detection of SARS-CoV-2 utilizing qRT-PCR in saliva obtained from asymptomatic or gentle COVID-19 sufferers, comparative evaluation with matched nasopharyngeal samples

Targets: The correct detection of extreme acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) is important for the prognosis of coronavirus illness 2019 (COVID-19). We in contrast the quantitative RT-PCR outcomes between nasopharyngeal swabs and saliva specimens.
Strategies: A COVID-19 outbreak occurred on a cruise ship at Nagasaki port, Japan. We obtained 123 nasopharyngeal swabs and saliva every from asymptomatic or gentle sufferers within the late section of an infection.
Outcomes: The intervals from the prognosis to the sampling had been 25.5 days for nasopharyngeal swabs and 28.9 days for saliva. The optimistic fee was 19.5% (24/123) for nasopharyngeal swabs and 38.2% (47/123) for saliva (P = 0.48). The quantified viral copies (imply ± SEM copies/5 μl) had been 9.3±2.6 in nasopharyngeal swabs and 920±850 in saliva (P = 0.0006).
Conclusions: Some great benefits of saliva specimens embrace optimistic fee enchancment and correct viral load detection. Saliva could also be used as a dependable pattern for SARS-CoV-2 detection.
russelldurrett
russelldurrett

Bovine Colon Tissue Lysate

IBOCLNTL100UG each
EUR 541
Description: Bovine Colon Tissue Lysate

cDNA - Rat Normal Tissue: Colon

C1434090 40 reactions
EUR 578

cDNA - Human Tumor Tissue: Colon

C1235090 40 reactions
EUR 578

cDNA - Mouse Normal Tissue: Colon

C1334090 40 reactions
EUR 578

cDNA - Human Adult Normal Tissue: Colon

C1234090 40 reactions
EUR 385

cDNA - Monkey (Rhesus) Normal Tissue: Colon

C1534090 40 reactions
EUR 385

cDNA - Human Diabetic Diseased Tissue: Colon

C1236090Dia 40 reactions
EUR 729

cDNA - Monkey (Cynomolgus) Normal Tissue: Colon

C1534090-Cy 40 reactions
EUR 385

Bovine Colon Cancer Antigen(CCA) ELISA Kit

NSL0064Bo 96 T
EUR 528

cDNA - Human Adult Normal Tissue: Colon Sigmoid

C1234095 40 reactions
EUR 385

cDNA - Monkey (Rhesus) Normal Tissue: Colon Sigmoid

C1534095 40 reactions
EUR 578

cDNA - Human Adult Normal Tissue: Colon Ascending

C1234091 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Colon Descending

C1234092 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Colon Transverse

C1234096 40 reactions
EUR 385

cDNA - Monkey (Rhesus) Normal Tissue: Colon Ascending

C1534091 40 reactions
EUR 578

cDNA - Monkey (Rhesus) Normal Tissue: Colon descending

C1534092 40 reactions
EUR 578

cDNA - Monkey (Rhesus) Normal Tissue: Colon Transverse

C1534096 40 reactions
EUR 578

Genorise® Bovine IL-1b cDNA Clone, 10 ug

GR152001 10 µg
EUR 153

Matched Pair - cDNA - Human Primary Tumor and Normal Tissue: Colon

C8235090-PP 10 reactions x2
EUR 530

Matched Pair - cDNA - Human Primary and Matched Metastatic Tumor Tissue: Colon

C8235090-PM 10 reactions x2
EUR 1102

Bovine colony-stimulating factor(CSF) ELISA Kit

NSL1488Bo 96 T
EUR 528

Colon

TMA2081 each
EUR 546
Description: Colon, prostate and breast tissue array, 156 cases/208 cores

Bovine Granulocyte colony-stimulating factor(CSF3) ELISA kit

CSB-EL006049BO-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Bovine Granulocyte colony-stimulating factor (CSF3) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Bovine Granulocyte colony-stimulating factor(CSF3) ELISA kit

1-CSB-EL006049BO
  • EUR 843.60
  • EUR 5811.60
  • EUR 3084.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Bovine Granulocyte colony-stimulating factor(CSF3) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Bovine Granulocyte colony-stimulating factor (CSF3) ELISA Kit

RK06106 96T
EUR 280

Bovine Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit

QY-E60021 96T
EUR 511.2

Bovine Granulocyte Colony Stimulating Factor(G-CSF) ELISA Kit

NSL0295Bo 96 T
EUR 528

Bovine Granulocyte macrophage colony stimulating factor, GM- CSF

ELA-E0045b 96 Tests
EUR 1113.6

Bovine Macrophage Colony-Stimulating Factor(M-CSF)ELISA Kit

NSL1682Bo 96 T
EUR 528

Colon cancer with colon tissue array

CO1021a each
EUR 306
Description: Colon cancer with colon tissue array, including pathology grade, with MMR (MLH1/PMS2/MSH2/MSH6) IHC results,TNM and clinical stage, containing 86 cases of adenocarcinoma, 4 papillary adenocarcinoma, 7 mucinous adenocarcinoma, 2 adenoma, 1 congenital megacolon, 2 colon tissue, 102 cases/102 cores replacing CO1021

Colon cancer with colon tissue array

CO1921 each
EUR 546
Description: Colon cancer with colon tissue array, including pathology grade, TNM and clinical stage, 192 cases/192 cores

Colon cancer with colon tissue array

CO2161b each
EUR 474
Description: Colon cancer with colon tissue array, including pathology grade, TNM and clinical stage, 216 cases/216 cores, replacing CO2161a

Colon cancer with colon tissue array

CO721 each
EUR 270
Description: Colon cancer with colon tissue array, including pathology grade, TNM and clinical stage, 24 cases/72 cores

Colon cancer with colon tissue array

CO805 each
EUR 258
Description: Colon cancer with colon tissue array, including pathology grade, TNM and clinical stage, 40 cases/80 cores

Colon adenocarcinoma with colon tissue array

CO483b each
EUR 198
Description: Colon adenocarcinoma with colon tissue array, including pathology, TNM and clinical stage, 48 cases/48 cores, replacing CO483a

Colon tissue array with colon cancer tissue

BN05014a each
EUR 222
Description: Colon tissue array with colon cancer tissue, including pathology grade, TNM and clinical stage, 24 cases/72 cores, replacing BN05014

Colon cancer with matched colon tissue array

CO1801a each
EUR 474
Description: Colon cancer with matched colon tissue array, including pathology grade,with MMR (MLH1/PMS2/MSH2/MSH6) IHC results, TNM and clinical stage, containing 90 cases of adenocarcinoma, and 90 cancer adjacent or adjacent normal colon tissue, 90 cases/180 cores, replacing CO1801

Colon cancer with matched colon tissue array

CO602a each
EUR 222
Description: Colon cancer with matched colon tissue array, including pathology grade, TNM and clinical stage, 30 cases/60 cores, replacing CO602

Colon cancer with matched colon tissue array

CO703 each
EUR 198
Description: Colon cancer with matched colon tissue array, including pathology grade, TNM and clinical stage, 35 cases/70 cores,replaced by CO703a

Colon Lysate

1472 0.1 mg
EUR 229.2
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Colon Lysate

21-179 0.1 mg
EUR 342.6
Description: Monkey (Cynomolgus) colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) colon tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Colon Lysate

21-288 0.1 mg
EUR 342.6
Description: Monkey (Rhesus) colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Rhesus) colon tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine)

4-PAA042Bo01
  • EUR 310.80
  • EUR 3232.80
  • EUR 800.40
  • EUR 391.20
  • EUR 261.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF)

Bovine GM-CSF(Granulocyte Macrophage Colony Stimulating Factor) ELISA Kit

EB0161 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Cattle;Sensitivity: 9.375pg/ml

Bovine Macrophage Colony-Stimulating Factor Receptor(M-CSFR) ELISA Kit

NSL0577Bo 96 T
EUR 528

Bovine Granulocyte-macrophage colony-stimulating factor(CSF2) ELISA kit

CSB-EL006045BO-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Bovine Granulocyte-macrophage colony-stimulating factor (CSF2) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Bovine Granulocyte-macrophage colony-stimulating factor(CSF2) ELISA kit

1-CSB-EL006045BO
  • EUR 1080.00
  • EUR 6571.20
  • EUR 3480.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Bovine Granulocyte-macrophage colony-stimulating factor(CSF2) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Bovine CSF2/ Granulocyte-macrophage colony-stimulating factor ELISA Kit

E0071Bo 1 Kit
EUR 860.4

Bovine Granulocyte-macrophage colony-stimulating factor (CSF2) ELISA Kit

RK06111 96T
EUR 280

Colon carcinoma

CO1005a each
EUR 306
Description: Colon carcinoma, metastatic carcinoma and colon adenoma tissue array with colon tissue as control, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 98 cases/100 cores, replacing CO1005

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), APC

4-PAA042Bo01-APC
  • EUR 435.60
  • EUR 4232.40
  • EUR 1170.00
  • EUR 558.00
  • EUR 271.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with APC.

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), Cy3

4-PAA042Bo01-Cy3
  • EUR 531.60
  • EUR 5593.20
  • EUR 1510.80
  • EUR 693.60
  • EUR 312.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with Cy3.

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), HRP

4-PAA042Bo01-HRP
  • EUR 397.20
  • EUR 3687.60
  • EUR 1034.40
  • EUR 502.80
  • EUR 255.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with HRP.

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), PE

4-PAA042Bo01-PE
  • EUR 372.00
  • EUR 3409.20
  • EUR 960.00
  • EUR 470.40
  • EUR 241.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with PE.

Bovine Granulocyte-Macrophage Colony Stimulating Factor(GM-CSF) ELISA Kit

NSL0320Bo 96 T
EUR 528

Colon adenocarcinoma tissue array with colon tissue

CO1201 each
EUR 348
Description: Colon adenocarcinoma tissue array with colon tissue, with pathology grade, TNM/Stage, 120 cases/120 cores (core size 1.0mm), replacing BC051110c

Innovative Grade US Origin Bovine Colostrum

IGBOBMKCOF1000ML each
EUR 210
Description: Innovative Grade US Origin Bovine Colostrum

Innovative Grade US Origin Bovine Colostrum

IGBOBMKCOZ1000ML each
EUR 210
Description: Innovative Grade US Origin Bovine Colostrum

cDNA - Plant Normal Tissue: cDNA - Plant: Corn

C1634330 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Rice

C1634360 40 reactions
EUR 674

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), FITC

4-PAA042Bo01-FITC
  • EUR 372.00
  • EUR 3409.20
  • EUR 960.00
  • EUR 470.40
  • EUR 241.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with FITC.

cDNA - Plant Normal Tissue: cDNA - Plant: Wheat

C1634390 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Orange

C1634340 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Potato

C1634350 40 reactions
EUR 674

Colon cancer tissue array with normal colon tissue

T054c each
EUR 48
Description: Colon cancer tissue array with normal colon tissue, including TNM, clinical stage and pathology grade, 12 cases/ 24 cores, replaced by T054d

High-density colon cancer with colon tissue array

CO6161 each
EUR 954
Description: High-density colon cancer with colon tissue array, including pathology grade, TNM and clinical stage, 322 cases/616 cores

Colon cancer with adjacent normal colon tissue array

CO2001a each
EUR 474
Description: Colon cancer with adjacent normal colon tissue array, including TNM and pathology grade with MMR (MLH1/PMS2/MSH2/MSH6) IHC results, containing 140 cases of adenocarcinoma, 52 cases of mucinous adenocarcinoma and 8 adjacent normal colon tissue, 200 cases/ 200 cores, replacing CO2001

Colon cancer with adjacent normal colon tissue array

CO806 each
EUR 270
Description: Colon cancer with adjacent normal colon tissue array, replacing BN05012, 80 cases/80 cores

cDNA - Plant Normal Tissue: cDNA - Plant: Arabidopsis

C1634310 40 reactions
EUR 674

Colon cancer tissue array with normal colon tissue and adjacent colon tissue as control

CO20811a each
EUR 546
Description: Colon cancer tissue array with normal colon tissue and adjacent colon tissue as control, including TNM and pathology grade with MMR (MLH1/PMS2/MSH2/MSH6) IHC results,208 cases/ 208 cores, replacing CO20811

Colon cancer with matched adjacent normal colon tissue

CO321a each
EUR 168
Description: Colon cancer with matched adjacent normal colon tissue, including pathology grade, TNM and clinical stage, 16 cases/32 cores, replacing CO321

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), APC-Cy7

4-PAA042Bo01-APC-Cy7
  • EUR 728.40
  • EUR 8320.80
  • EUR 2197.20
  • EUR 972.00
  • EUR 399.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with APC-Cy7.

cDNA - Plant Normal Tissue: cDNA - Plant: Soy bean

C1634370 40 reactions
EUR 674

Colon disease spectrum (colon cancer progression) tissue array

CO808 each
EUR 306
Description: Colon disease spectrum (colon cancer progression) tissue array, including pathology grade, TNM and clinical stage, 76 cases/80 cores

Colon adenocarcinoma with matched adjacent colon tissue array

CO487 each
EUR 168
Description: Colon adenocarcinoma with matched adjacent colon tissue array, including TNM and pathology grade, 16 cases/48 cores

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), Biotinylated

4-PAA042Bo01-Biotin
  • EUR 388.80
  • EUR 3172.80
  • EUR 927.60
  • EUR 478.80
  • EUR 268.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with Biotin.

Multiple types of colon disease with colon tissue array

CO245a each
EUR 66
Description: Multiple types of colon disease with colon tissue array, including crohn‘s disease, chronic inflammation of the colonic mucosa, ulcerative colitis, adenocarcinoma, 12 cases/24 cores (core size 1.5mm), replacing CO245

Different stages of colon cancer and colon tissue array

CO807 each
EUR 306
Description: Different stages of colon cancer and colon tissue array, including pathology grade, TNM and clinical satge, 80 cases/80 cores

Progression of colon disease (IBD) with colon tissue array

CO246 each
EUR 66
Description: Progression of colon disease (IBD) with colon tissue array, 5 cases of crohn's disease, 2 ulcerative colitis, 5 normal colon tissue, 12 cases/24 cores

High-density colon cancer and normal colon tissue array

CO208a each
EUR 546
Description: High-density colon cancer and normal colon tissue array, 64 cases/192 cores, including TNM and pathology grade, 64 cases/192 cores (core size 1.0mm), replaced by CO208b

Colon Lupus Lysate

XBL-10335 0.1 mg
EUR 796.2
Description: Human colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human colon tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Fetal Colon Lysate

XBL-10403 0.1 mg
EUR 342.6
Description: Fetal human colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human colon tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Colon cancer with colon tissue array (2010 WHO classification)

CO701a each
EUR 306
Description: Colon cancer with colon tissue array (2010 WHO classification), including pathology grade, TNM and clinical satge (AJCC 7th edition), 70 cases/70 cores, replacing CO701

Colon cancer tissue array with adjacent normal colon tissue

CO241a each
EUR 66
Description: Colon cancer tissue array with adjacent normal colon tissue, including TNM, clinical stage and pathology grade, 6 cases/24 cores

Bovine Granulocyte-Macrophage Colony Stimulating Factor Anti-body(GM-CSF Ab) ELISA Kit

NSL0319Bo 96 T
EUR 528

Bovine COL11α1 ELISA Kit

EBC0349 96Tests
EUR 625.2

Colon Membrane Lysate

XBL-10505 0.1 mg
EUR 619.8
Description: Human colon tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human colon tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated colon tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated colon tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Bovine COL10 ELISA Kit

EBC0336 96Tests
EUR 625.2

Novo? cDNA Kit

M1165-100 each
EUR 424.8

Novo? cDNA Kit

M1165-25 each
EUR 320.4

Bovine COL1 ELISA Kit

EBC0021 96Tests
EUR 625.2

Bovine COL3 ELISA Kit

EBC0024 96Tests
EUR 625.2

Bovine COL4 ELISA Kit

EBC0054 96Tests
EUR 625.2

Bovine COL7 ELISA Kit

EBC0333 96Tests
EUR 625.2

Bovine COL2 ELISA Kit

EBC0786 96Tests
EUR 625.2

cDNA - Lupus: Lung

C1236152Lup 40 reactions
EUR 737

Colon adenocarcinoma TMA

CO571 each
EUR 270
Description: Colon adenocarcinoma TMA, 5 samples have matched cancer adjacent tissue samples, including pathology grade, TNM and clinical stage (reference AJCC 8th version) with MMR (MLH1/PMS2/MSH2/MSH6) IHC results, 52 cases/ 57 cores (core size 1.5mm) Recent Year Collection

Colon Cytoplasmic Lysate

XBL-10504 0.1 mg
EUR 273.3
Description: Human colon tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human colon tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated colon tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated colon tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

Normal colon tissue array with colon cancer tissue as control

CO727 each
EUR 270
Description: Normal colon tissue array with colon cancer tissue as control, including TNM, clinical stage and pathology grade, 24 cases/ 72 cores

Bovine anti-Granulocyte-Macrophage Colony Stimulating Factor antibody(GM-CSF Ab)ELISA Kit

QY-E60012 96T
EUR 511.2

cDNA - Lupus: Heart

C1236122Lup 40 reactions
EUR 737

cDNA - Lupus: Liver

C1236149Lup 40 reactions
EUR 737

cDNA - Asthma: Lung

C1236152Ld-1 40 reactions
EUR 899

Bovine COL1a2 ELISA Kit

EBC0329 96Tests
EUR 625.2

Bovine COL2a1 ELISA Kit

EBC0330 96Tests
EUR 625.2

Bovine COL3a1 ELISA Kit

EBC0331 96Tests
EUR 625.2

Bovine COL6a1 ELISA Kit

EBC0332 96Tests
EUR 625.2

Bovine COL8a1 ELISA Kit

EBC0334 96Tests
EUR 625.2

Bovine COL9a1 ELISA Kit

EBC0337 96Tests
EUR 625.2

cDNA - Lupus: Kidney

C1236142Lup 40 reactions
EUR 737

cDNA - Lupus: Spleen

C1236246Lup 40 reactions
EUR 737

Evo? cDNA Supermix

M1168-100 each
EUR 457.2

Evo? cDNA Supermix

M1168-25 each
EUR 320.4

Bovine COL4A3BP ELISA KIT

ELI-49322b 96 Tests
EUR 1113.6

cDNA - Lupus: stomach

C1236248Lup 40 reactions
EUR 737

Novo? cDNA Supermix

M1169-100 each
EUR 529.2

Novo? cDNA Supermix

M1169-25 each
EUR 346.8

Colon cancer tissue with matched adjacent normal colon tissue array

BC05118d each
EUR 306
Description: Colon cancer tissue with matched adjacent normal colon tissue array, including TNM, clinical stage and pathology grade, 50 cases/100 cores

Colon cancer tissue array with matched adjacent normal colon tissue

CO801a each
EUR 270
Description: Colon cancer tissue array with matched adjacent normal colon tissue, including TNM, clinical stage and pathology grade, 40 cases/ 80 cores, replced by CO801b

cDNA - Emphysema: Lung

C1236152Ld-3 40 reactions
EUR 899

cDNA - Pneumonia: Lung

C1236152Ld-4 40 reactions
EUR 899

cDNA - Lupus: Pancreas

C1236188Lup 40 reactions
EUR 737

Colon cancer with matched colon tissue array (most in grade II)

CO703a each
EUR 198
Description: Colon cancer with matched colon tissue array (most in grade II), including pathology grade, TNM and clinical stage, 35 cases/70 cores

Bovine Collagen Purified

IBOCLG10GM each
EUR 334
Description: Bovine Collagen Purified

Bovine Collagen Purified

IBOCLG1GM each
EUR 100
Description: Bovine Collagen Purified

Bovine Collagen Purified

IBOCLG5GM each
EUR 196
Description: Bovine Collagen Purified

cDNA - Bronchitis: Lung

C1236152Ld-2 40 reactions
EUR 899

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems
  • EUR 744.00
  • EUR 627.60
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems
  • EUR 1045.20
  • EUR 794.40
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Bovine ColIV ELISA Kit

EBC0787 96Tests
EUR 625.2

Total RNA - Lupus: Colon

R1236090Lup-50 50 ug
EUR 421.2

Progressive colon adenocarcinoma

CO1506 each
EUR 258
Description: Progressive colon adenocarcinoma, matched cancer adjacent with normal adjacent tissue array, including pathology grade, TNM /Stage ( AJCC 8th version) and MMR (MLH1/PMS2/MSH2/MSH6) IHC results, 50 cases/150cores (core size 1.0mm),Recent Year Collection

Type 1 Bovine Collagen

TBC320 20ml @ 3mg/ml
EUR 418.8

cDNA - hypertension: Vein

C1236020Hd-2 40 reactions
EUR 729

Bovine COLXVII ELISA Kit

EBC0335 96Tests
EUR 625.2

Bovine Colicin ELISA Kit

EBC0793 96Tests
EUR 625.2

Bovine Colicin ELISA Kit

NSL0108Bo 96 T
EUR 528

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 678.00
  • EUR 577.20
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

cDNA - hypertension: Heart

C1236122Hd-2 40 reactions
EUR 729

Rat Colon Tissue Lysate

IRTCLNTL100UG each
EUR 245
Description: Rat Colon Tissue Lysate

Evo™ cDNA Kit

M1164-100 each Ask for price

Evo™ cDNA Kit

M1164-25 each Ask for price

cDNA - hypertension: Artery

C1236013Hd-2 40 reactions
EUR 729

cDNA - hypertension: Kidney

C1236142Hd-2 40 reactions
EUR 729

Human Colon Tumor lystae

HTL-1327 100ug
EUR 343.2

Bovine Col I ELISA Kit

EBC0788 96Tests
EUR 625.2

Colon carcinoma tissue array with adjacent normal colon tissue as control

CO242b each
EUR 66
Description: Colon carcinoma tissue array with adjacent normal colon tissue as control, including TNM, clinical stage and pathology grade, 12 cases/24 cores, replacing CO242a

Colon Tissue Lysate (Tumor)

1716-01 0.1 mg
EUR 336.3
Description: Colon tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Tumor)

1716-02 0.1 mg
EUR 336.3
Description: Colon tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Tumor)

1716-03 0.1 mg
EUR 336.3
Description: Colon tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Tumor)

1716-04 0.1 mg
EUR 336.3
Description: Colon tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon cancer tissue array

CRM1503 each
EUR 420
Description: Colon cancer tissue array, 150 cases/150 cores

Colon cancer tissue array

CRM1504 each
EUR 420
Description: Colon cancer tissue array, 150 cases/150 cores

Colon cancer tissue array

CRM1506 each
EUR 420
Description: Colon cancer tissue array, 150 cases/150 cores

Colon cancer tissue array

CRM1507 each
EUR 420
Description: Colon cancer tissue array, 150 cases/150 cores

Colon cancer tissue array

CO1502 each
EUR 258
Description: Colon cancer tissue array, including TNM, clinical stage and pathology grade, 75 cases/150 cores

Colon cancer tissue array

CO1503 each
EUR 258
Description: Colon cancer tissue array, including TNM, clinical stage and pathology grade, 75 cases/150 cores

Colon cancer tissue array

CO244 each
EUR 270
Description: Colon cancer tissue array, with normal tissue, including TNM and pathology grade, 2 serial sections, 24 cases/48 cores

Colon cancer tissue array

CO485 each
EUR 270
Description: Colon cancer tissue array, including TNM, clinical stage and pathology grade, 48 cases/48 cores

Colon cancer tissue array

CO486a each
EUR 414
Description: Colon cancer tissue array, including pathology grade, TNM and clinical stage, 2 serial sections, 48 cases/96 cores, replacing CO486

Colon cancer tissue array

CO641 each
EUR 258
Description: Colon cancer tissue array, including TNM, stage and pathology grade, 64 cases/ 64 cores

Colon cancer tissue array

CO804b each
EUR 270
Description: Colon cancer tissue array, including pathology grade, TNM and clinical stage, 80 cases/80 cores, replcing CO804a

Colon cancer tissue array

CO901a each
EUR 306
Description: Colon cancer tissue array, with adjacent normal colon tissue, including TNM, clinical stage and pathology grade, 50 cases/90 cores, replacing CO901

Colon cancer tissue array

CO961 each
EUR 258
Description: Colon cancer tissue array, including TNM, clinical stage and pathology grade, 48 cases/96 cores

Colon cancer tissue array

CO962 each
EUR 258
Description: Colon cancer tissue array, including pathology grade, TNM/Stage, 48 cases/96 cores

Total Protein - Lupus: Colon

P1236090Lup 1 mg
EUR 486

Human Colon Tissue Lysate

IHUCLNTL100UG each
EUR 361
Description: Human Colon Tissue Lysate

Mouse Colon Tissue Lysate

IMSCLNTL100UG each
EUR 245
Description: Mouse Colon Tissue Lysate

Bovine Col III ELISA Kit

EBC0792 96Tests
EUR 625.2

Colon Tissue Lysate (Normal)

1715-01 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-02 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-03 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-04 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-05 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-06 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-07 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Comparative Analysis of Two Automated Multiplex RT-PCR Assessments for Fast Detection of Influenza and Respiratory Syncytial Viruses

Background: Fast and correct prognosis of influenza virus (Flu) and respiratory syncytial virus (RSV) is vital for managing each the affected person and laboratory. We in contrast the cobas Influenza A/B & RSV assay (cobas Liat) with the Simplexa Flu A/B & RSV assay (Simplexa) to guage which check methodology is extra advantageous contemplating the sources of the laboratory and outcomes of check efficiency.
Strategies: A complete of 236 respiratory specimens from sufferers referred for respiratory virus testing had been retrospectively evaluated; 53 specimens examined optimistic for every of Flu A, Flu B, and RSV, and 77 specimens examined unfavorable based mostly on the outcomes of the reference methodology, i.e., the Seegene Allplex Respiratory Panel half/3 (Seegene, Seoul, Korea). The turnaround time (TAT) was 20 minutes per specimen for cobas Liat and 78 minutes per eight speci-mens for Simplexa. The overall hands-on time was round one minute per specimen for each assessments. The specimen quantity required for testing was 200 µL for cobas Liat and 50 µL for Simplexa. Seegene Allplex Respiratory Panel half/Three was used because the reference methodology.
Outcomes: The variety of invalid outcomes was 1 (0.4%) for cobas Liat and 10 (4.2%) for Simplexa (p < 0.05). All outcomes had been according to these of the reference methodology in cobas Liat. The sensitivity and specificity for Flu A, Flu B, and RSVA had been 100% with Simplexa. Nevertheless, the sensitivity for RSVB was 80.0% with Simplexa, which was a statistically vital distinction with the discovering for cobas Liat (p < 0.05). Comparability of the cycle threshold (Ct) values of RSV for Simplexa with the reference methodology confirmed correlation as steady variables (p < 0.001) with a better propensity for acquiring Ct values with Simplexa, the exception being the six false unfavorable outcomes; their Ct values had been greater than 30 within the reference methodology.
Conclusions: Cobas Liat confirmed correct efficiency with a fast TAT and an excellent workflow effectivity. Cobas Liat is extra environment friendly than Simplexa as a point-of-care check for the detection of RSV.

Disagreement between PCR and serological prognosis of Trypanosoma cruzi an infection in blood donors from a Colombian endemic area

Introduction: Chagas’ illness is the main reason behind infectious myocarditis worldwide. This an infection brought on by Trypanosoma cruzi is often life-long and asymptomatic; nonetheless, the third a part of contaminated folks can develop extreme and even deadly cardiomyopathy. Because the parasitemia within the persistent section is each low-grade and intermittent, T. cruzi an infection is principally detected by serology, though this methodology has sensitivity and specificity limitations.
Goal: To find out the extent of settlement between serologic and molecular assessments in 658 voluntary blood donors from six provinces within the Colombian division of Santander.
Supplies and strategies: We evaluated an array of diagnostic applied sciences by cross-section sampling performing a serological double diagnostic check for T. cruzi antibody detection (Chagas III ELISA™, BiosChile Group, and ARCHITECT Chagas CMIA™, Abbott), and DNA detection by polymerase chain response (PCR). We collected the demographic, medical, and epidemiological data of contributors. The pattern measurement was calculated utilizing Epidat™ and the statistical evaluation was finished with Stata 12.1™.
Outcomes: PCR was six occasions extra delicate in detecting T. cruzi an infection than ELISA/CMIA with prevalence values of 1.8% (12/658) and 0.3% (2/658), respectively, and kappa=0.28 (95%CI: -0.03 – 0.59). In distinction, serology confirmed a sensitivity of 16.7% (95%CI: 2.09 – 48.4) and a specificity of 100% (95%CI: 99.4 – 100). All seropositive samples had been discovered to be optimistic by PCR.
Conclusions: The implementation of PCR as a complementary methodology for screening donors might scale back the likelihood of false unfavorable and the resultant danger of transfusional-transmission of Chagas’ illness, particularly in endemic areas.

Simultaneous screening of the FRAXA and FRAXE loci for fast detection of FMR1 CGG and/or AFF2 CCG repeat expansions by triplet-primed PCR

Reasonable to hyper growth of trinucleotide repeats on the FRAXA and FRAXE fragile websites, with or with out concomitant hypermethylation are related to mental incapacity and different situations. Not like molecular prognosis of FMR1 CGG repeat expansions in FRAXA, present detection of AFF2 CCG repeat expansions in FRAXE depends on low-throughput and inefficient strategies combining Southern blot and PCR. A novel triplet-primed PCR assay was developed to concurrently display for trinucleotide repeat expansions on the FRAXA and FRAXE fragile websites, and validated utilizing archived medical samples of identified FMR1 and AFF2 genotypes.
Sequencing of inhabitants samples and FRAXE-affected samples was carried out to guage variation of the AFF2 CCG repeat construction. The duplex assay precisely recognized expansions on the FMR1 and AFF2 trinucleotide repeat loci. Sanger sequencing of the AFF2 CCG repeat revealed that the SNP variant rs868914124(C), which successfully provides two CCG repeats at the 5′ finish, is enriched within the Malay inhabitants and with brief repeats (<11 CCGs), and was current in all six expanded AFF2 alleles of this research. All expanded AFF2 alleles contained a number of non-CCG interruptions in the direction of the 5′ finish of the repeat. We’ve developed a delicate, sturdy and fast assay to concurrently detect growth mutations on the FMR1 and AFF2 trinucleotide repeat loci, simplifying the screening of FRAXA and FRAXE related issues.

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