Detection of SARS-CoV-2 utilizing qRT-PCR in saliva obtained from asymptomatic or gentle COVID-19 sufferers, comparative evaluation with matched nasopharyngeal samples
Targets: The correct detection of extreme acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) is important for the prognosis of coronavirus illness 2019 (COVID-19). We in contrast the quantitative RT-PCR outcomes between nasopharyngeal swabs and saliva specimens.
Strategies: A COVID-19 outbreak occurred on a cruise ship at Nagasaki port, Japan. We obtained 123 nasopharyngeal swabs and saliva every from asymptomatic or gentle sufferers within the late section of an infection.
Outcomes: The intervals from the prognosis to the sampling had been 25.5 days for nasopharyngeal swabs and 28.9 days for saliva. The optimistic fee was 19.5% (24/123) for nasopharyngeal swabs and 38.2% (47/123) for saliva (P = 0.48). The quantified viral copies (imply ± SEM copies/5 μl) had been 9.3±2.6 in nasopharyngeal swabs and 920±850 in saliva (P = 0.0006).
Conclusions: Some great benefits of saliva specimens embrace optimistic fee enchancment and correct viral load detection. Saliva could also be used as a dependable pattern for SARS-CoV-2 detection.

Bovine Colon Tissue Lysate |
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IBOCLNTL100UG | Innovative research | each | EUR 541 |
Description: Bovine Colon Tissue Lysate |
cDNA - Rat Normal Tissue: Colon |
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C1434090 | Biochain | 40 reactions | EUR 578 |
cDNA - Human Tumor Tissue: Colon |
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C1235090 | Biochain | 40 reactions | EUR 578 |
cDNA - Mouse Normal Tissue: Colon |
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C1334090 | Biochain | 40 reactions | EUR 578 |
cDNA - Human Adult Normal Tissue: Colon |
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C1234090 | Biochain | 40 reactions | EUR 385 |
cDNA - Monkey (Rhesus) Normal Tissue: Colon |
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C1534090 | Biochain | 40 reactions | EUR 385 |
cDNA - Human Diabetic Diseased Tissue: Colon |
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C1236090Dia | Biochain | 40 reactions | EUR 729 |
cDNA - Monkey (Cynomolgus) Normal Tissue: Colon |
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C1534090-Cy | Biochain | 40 reactions | EUR 385 |
Bovine Colon Cancer Antigen(CCA) ELISA Kit |
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NSL0064Bo | Sunlong | 96 T | EUR 528 |
cDNA - Human Adult Normal Tissue: Colon Sigmoid |
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C1234095 | Biochain | 40 reactions | EUR 385 |
cDNA - Monkey (Rhesus) Normal Tissue: Colon Sigmoid |
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C1534095 | Biochain | 40 reactions | EUR 578 |
cDNA - Human Adult Normal Tissue: Colon Ascending |
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C1234091 | Biochain | 40 reactions | EUR 385 |
cDNA - Human Adult Normal Tissue: Colon Descending |
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C1234092 | Biochain | 40 reactions | EUR 385 |
cDNA - Human Adult Normal Tissue: Colon Transverse |
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C1234096 | Biochain | 40 reactions | EUR 385 |
cDNA - Monkey (Rhesus) Normal Tissue: Colon Ascending |
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C1534091 | Biochain | 40 reactions | EUR 578 |
cDNA - Monkey (Rhesus) Normal Tissue: Colon descending |
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C1534092 | Biochain | 40 reactions | EUR 578 |
cDNA - Monkey (Rhesus) Normal Tissue: Colon Transverse |
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C1534096 | Biochain | 40 reactions | EUR 578 |
Genorise® Bovine IL-1b cDNA Clone, 10 ug |
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GR152001 | Genorise Scientific | 10 µg | EUR 153 |
Matched Pair - cDNA - Human Primary Tumor and Normal Tissue: Colon |
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C8235090-PP | Biochain | 10 reactions x2 | EUR 530 |
Matched Pair - cDNA - Human Primary and Matched Metastatic Tumor Tissue: Colon |
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C8235090-PM | Biochain | 10 reactions x2 | EUR 1102 |
Bovine colony-stimulating factor(CSF) ELISA Kit |
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NSL1488Bo | Sunlong | 96 T | EUR 528 |
Colon |
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TMA2081 | TissueArray | each | EUR 546 |
Description: Colon, prostate and breast tissue array, 156 cases/208 cores |
Bovine Granulocyte colony-stimulating factor(CSF3) ELISA kit |
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CSB-EL006049BO-24T | Cusabio | 1 plate of 24 wells | EUR 198 |
Description: Quantitativesandwich ELISA kit for measuring Bovine Granulocyte colony-stimulating factor (CSF3) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Bovine Granulocyte colony-stimulating factor(CSF3) ELISA kit |
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1-CSB-EL006049BO | Cusabio |
|
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Description: Quantitativesandwich ELISA kit for measuring Bovine Granulocyte colony-stimulating factor(CSF3) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Bovine Granulocyte colony-stimulating factor (CSF3) ELISA Kit |
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RK06106 | Abclonal | 96T | EUR 280 |
Bovine Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit |
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QY-E60021 | Qayee Biotechnology | 96T | EUR 511.2 |
Bovine Granulocyte Colony Stimulating Factor(G-CSF) ELISA Kit |
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NSL0295Bo | Sunlong | 96 T | EUR 528 |
Bovine Granulocyte macrophage colony stimulating factor, GM- CSF |
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ELA-E0045b | Lifescience Market | 96 Tests | EUR 1113.6 |
Bovine Macrophage Colony-Stimulating Factor(M-CSF)ELISA Kit |
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NSL1682Bo | Sunlong | 96 T | EUR 528 |
Colon cancer with colon tissue array |
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CO1021a | TissueArray | each | EUR 306 |
Description: Colon cancer with colon tissue array, including pathology grade, with MMR (MLH1/PMS2/MSH2/MSH6) IHC results,TNM and clinical stage, containing 86 cases of adenocarcinoma, 4 papillary adenocarcinoma, 7 mucinous adenocarcinoma, 2 adenoma, 1 congenital megacolon, 2 colon tissue, 102 cases/102 cores replacing CO1021 |
Colon cancer with colon tissue array |
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CO1921 | TissueArray | each | EUR 546 |
Description: Colon cancer with colon tissue array, including pathology grade, TNM and clinical stage, 192 cases/192 cores |
Colon cancer with colon tissue array |
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CO2161b | TissueArray | each | EUR 474 |
Description: Colon cancer with colon tissue array, including pathology grade, TNM and clinical stage, 216 cases/216 cores, replacing CO2161a |
Colon cancer with colon tissue array |
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CO721 | TissueArray | each | EUR 270 |
Description: Colon cancer with colon tissue array, including pathology grade, TNM and clinical stage, 24 cases/72 cores |
Colon cancer with colon tissue array |
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CO805 | TissueArray | each | EUR 258 |
Description: Colon cancer with colon tissue array, including pathology grade, TNM and clinical stage, 40 cases/80 cores |
Colon adenocarcinoma with colon tissue array |
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CO483b | TissueArray | each | EUR 198 |
Description: Colon adenocarcinoma with colon tissue array, including pathology, TNM and clinical stage, 48 cases/48 cores, replacing CO483a |
Colon tissue array with colon cancer tissue |
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BN05014a | TissueArray | each | EUR 222 |
Description: Colon tissue array with colon cancer tissue, including pathology grade, TNM and clinical stage, 24 cases/72 cores, replacing BN05014 |
Colon cancer with matched colon tissue array |
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CO1801a | TissueArray | each | EUR 474 |
Description: Colon cancer with matched colon tissue array, including pathology grade,with MMR (MLH1/PMS2/MSH2/MSH6) IHC results, TNM and clinical stage, containing 90 cases of adenocarcinoma, and 90 cancer adjacent or adjacent normal colon tissue, 90 cases/180 cores, replacing CO1801 |
Colon cancer with matched colon tissue array |
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CO602a | TissueArray | each | EUR 222 |
Description: Colon cancer with matched colon tissue array, including pathology grade, TNM and clinical stage, 30 cases/60 cores, replacing CO602 |
Colon cancer with matched colon tissue array |
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CO703 | TissueArray | each | EUR 198 |
Description: Colon cancer with matched colon tissue array, including pathology grade, TNM and clinical stage, 35 cases/70 cores,replaced by CO703a |
Colon Lysate |
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1472 | ProSci | 0.1 mg | EUR 229.2 |
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Colon Lysate |
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21-179 | ProSci | 0.1 mg | EUR 342.6 |
Description: Monkey (Cynomolgus) colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) colon tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Colon Lysate |
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21-288 | ProSci | 0.1 mg | EUR 342.6 |
Description: Monkey (Rhesus) colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Rhesus) colon tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine) |
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4-PAA042Bo01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF) |
Bovine GM-CSF(Granulocyte Macrophage Colony Stimulating Factor) ELISA Kit |
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EB0161 | FN Test | 96T | EUR 681.12 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Cattle;Sensitivity: 9.375pg/ml |
Bovine Macrophage Colony-Stimulating Factor Receptor(M-CSFR) ELISA Kit |
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NSL0577Bo | Sunlong | 96 T | EUR 528 |
Bovine Granulocyte-macrophage colony-stimulating factor(CSF2) ELISA kit |
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CSB-EL006045BO-24T | Cusabio | 1 plate of 24 wells | EUR 198 |
Description: Quantitativesandwich ELISA kit for measuring Bovine Granulocyte-macrophage colony-stimulating factor (CSF2) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Bovine Granulocyte-macrophage colony-stimulating factor(CSF2) ELISA kit |
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1-CSB-EL006045BO | Cusabio |
|
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Description: Quantitativesandwich ELISA kit for measuring Bovine Granulocyte-macrophage colony-stimulating factor(CSF2) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Bovine CSF2/ Granulocyte-macrophage colony-stimulating factor ELISA Kit |
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E0071Bo | Sunlong | 1 Kit | EUR 860.4 |
Bovine Granulocyte-macrophage colony-stimulating factor (CSF2) ELISA Kit |
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RK06111 | Abclonal | 96T | EUR 280 |
Colon carcinoma |
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CO1005a | TissueArray | each | EUR 306 |
Description: Colon carcinoma, metastatic carcinoma and colon adenoma tissue array with colon tissue as control, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 98 cases/100 cores, replacing CO1005 |
Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), APC |
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4-PAA042Bo01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with APC. |
Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), Cy3 |
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4-PAA042Bo01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with Cy3. |
Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), HRP |
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4-PAA042Bo01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with HRP. |
Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), PE |
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4-PAA042Bo01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with PE. |
Bovine Granulocyte-Macrophage Colony Stimulating Factor(GM-CSF) ELISA Kit |
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NSL0320Bo | Sunlong | 96 T | EUR 528 |
Colon adenocarcinoma tissue array with colon tissue |
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CO1201 | TissueArray | each | EUR 348 |
Description: Colon adenocarcinoma tissue array with colon tissue, with pathology grade, TNM/Stage, 120 cases/120 cores (core size 1.0mm), replacing BC051110c |
Innovative Grade US Origin Bovine Colostrum |
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IGBOBMKCOF1000ML | Innovative research | each | EUR 210 |
Description: Innovative Grade US Origin Bovine Colostrum |
Innovative Grade US Origin Bovine Colostrum |
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IGBOBMKCOZ1000ML | Innovative research | each | EUR 210 |
Description: Innovative Grade US Origin Bovine Colostrum |
cDNA - Plant Normal Tissue: cDNA - Plant: Corn |
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C1634330 | Biochain | 40 reactions | EUR 674 |
cDNA - Plant Normal Tissue: cDNA - Plant: Rice |
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C1634360 | Biochain | 40 reactions | EUR 674 |
Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), FITC |
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4-PAA042Bo01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with FITC. |
cDNA - Plant Normal Tissue: cDNA - Plant: Wheat |
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C1634390 | Biochain | 40 reactions | EUR 674 |
cDNA - Plant Normal Tissue: cDNA - Plant: Orange |
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C1634340 | Biochain | 40 reactions | EUR 674 |
cDNA - Plant Normal Tissue: cDNA - Plant: Potato |
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C1634350 | Biochain | 40 reactions | EUR 674 |
Colon cancer tissue array with normal colon tissue |
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T054c | TissueArray | each | EUR 48 |
Description: Colon cancer tissue array with normal colon tissue, including TNM, clinical stage and pathology grade, 12 cases/ 24 cores, replaced by T054d |
High-density colon cancer with colon tissue array |
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CO6161 | TissueArray | each | EUR 954 |
Description: High-density colon cancer with colon tissue array, including pathology grade, TNM and clinical stage, 322 cases/616 cores |
Colon cancer with adjacent normal colon tissue array |
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CO2001a | TissueArray | each | EUR 474 |
Description: Colon cancer with adjacent normal colon tissue array, including TNM and pathology grade with MMR (MLH1/PMS2/MSH2/MSH6) IHC results, containing 140 cases of adenocarcinoma, 52 cases of mucinous adenocarcinoma and 8 adjacent normal colon tissue, 200 cases/ 200 cores, replacing CO2001 |
Colon cancer with adjacent normal colon tissue array |
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CO806 | TissueArray | each | EUR 270 |
Description: Colon cancer with adjacent normal colon tissue array, replacing BN05012, 80 cases/80 cores |
cDNA - Plant Normal Tissue: cDNA - Plant: Arabidopsis |
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C1634310 | Biochain | 40 reactions | EUR 674 |
Colon cancer tissue array with normal colon tissue and adjacent colon tissue as control |
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CO20811a | TissueArray | each | EUR 546 |
Description: Colon cancer tissue array with normal colon tissue and adjacent colon tissue as control, including TNM and pathology grade with MMR (MLH1/PMS2/MSH2/MSH6) IHC results,208 cases/ 208 cores, replacing CO20811 |
Colon cancer with matched adjacent normal colon tissue |
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CO321a | TissueArray | each | EUR 168 |
Description: Colon cancer with matched adjacent normal colon tissue, including pathology grade, TNM and clinical stage, 16 cases/32 cores, replacing CO321 |
Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), APC-Cy7 |
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4-PAA042Bo01-APC-Cy7 | Cloud-Clone |
|
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Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with APC-Cy7. |
cDNA - Plant Normal Tissue: cDNA - Plant: Soy bean |
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C1634370 | Biochain | 40 reactions | EUR 674 |
Colon disease spectrum (colon cancer progression) tissue array |
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CO808 | TissueArray | each | EUR 306 |
Description: Colon disease spectrum (colon cancer progression) tissue array, including pathology grade, TNM and clinical stage, 76 cases/80 cores |
Colon adenocarcinoma with matched adjacent colon tissue array |
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CO487 | TissueArray | each | EUR 168 |
Description: Colon adenocarcinoma with matched adjacent colon tissue array, including TNM and pathology grade, 16 cases/48 cores |
Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), Biotinylated |
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4-PAA042Bo01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with Biotin. |
Multiple types of colon disease with colon tissue array |
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CO245a | TissueArray | each | EUR 66 |
Description: Multiple types of colon disease with colon tissue array, including crohn‘s disease, chronic inflammation of the colonic mucosa, ulcerative colitis, adenocarcinoma, 12 cases/24 cores (core size 1.5mm), replacing CO245 |
Different stages of colon cancer and colon tissue array |
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CO807 | TissueArray | each | EUR 306 |
Description: Different stages of colon cancer and colon tissue array, including pathology grade, TNM and clinical satge, 80 cases/80 cores |
Progression of colon disease (IBD) with colon tissue array |
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CO246 | TissueArray | each | EUR 66 |
Description: Progression of colon disease (IBD) with colon tissue array, 5 cases of crohn's disease, 2 ulcerative colitis, 5 normal colon tissue, 12 cases/24 cores |
High-density colon cancer and normal colon tissue array |
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CO208a | TissueArray | each | EUR 546 |
Description: High-density colon cancer and normal colon tissue array, 64 cases/192 cores, including TNM and pathology grade, 64 cases/192 cores (core size 1.0mm), replaced by CO208b |
Colon Lupus Lysate |
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XBL-10335 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human colon tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Fetal Colon Lysate |
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XBL-10403 | ProSci | 0.1 mg | EUR 342.6 |
Description: Fetal human colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human colon tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Colon cancer with colon tissue array (2010 WHO classification) |
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CO701a | TissueArray | each | EUR 306 |
Description: Colon cancer with colon tissue array (2010 WHO classification), including pathology grade, TNM and clinical satge (AJCC 7th edition), 70 cases/70 cores, replacing CO701 |
Colon cancer tissue array with adjacent normal colon tissue |
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CO241a | TissueArray | each | EUR 66 |
Description: Colon cancer tissue array with adjacent normal colon tissue, including TNM, clinical stage and pathology grade, 6 cases/24 cores |
Bovine Granulocyte-Macrophage Colony Stimulating Factor Anti-body(GM-CSF Ab) ELISA Kit |
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NSL0319Bo | Sunlong | 96 T | EUR 528 |
Bovine COL11α1 ELISA Kit |
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EBC0349 | Abclonal | 96Tests | EUR 625.2 |
Colon Membrane Lysate |
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XBL-10505 | ProSci | 0.1 mg | EUR 619.8 |
Description: Human colon tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human colon tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated colon tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated colon tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
Bovine COL10 ELISA Kit |
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EBC0336 | Abclonal | 96Tests | EUR 625.2 |
Novo? cDNA Kit |
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M1165-100 | Biovision | each | EUR 424.8 |
Novo? cDNA Kit |
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M1165-25 | Biovision | each | EUR 320.4 |
Bovine COL1 ELISA Kit |
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EBC0021 | Abclonal | 96Tests | EUR 625.2 |
Bovine COL3 ELISA Kit |
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EBC0024 | Abclonal | 96Tests | EUR 625.2 |
Bovine COL4 ELISA Kit |
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EBC0054 | Abclonal | 96Tests | EUR 625.2 |
Bovine COL7 ELISA Kit |
|||
EBC0333 | Abclonal | 96Tests | EUR 625.2 |
Bovine COL2 ELISA Kit |
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EBC0786 | Abclonal | 96Tests | EUR 625.2 |
cDNA - Lupus: Lung |
|||
C1236152Lup | Biochain | 40 reactions | EUR 737 |
Colon adenocarcinoma TMA |
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CO571 | TissueArray | each | EUR 270 |
Description: Colon adenocarcinoma TMA, 5 samples have matched cancer adjacent tissue samples, including pathology grade, TNM and clinical stage (reference AJCC 8th version) with MMR (MLH1/PMS2/MSH2/MSH6) IHC results, 52 cases/ 57 cores (core size 1.5mm) Recent Year Collection |
Colon Cytoplasmic Lysate |
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XBL-10504 | ProSci | 0.1 mg | EUR 273.3 |
Description: Human colon tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human colon tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated colon tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated colon tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot. |
Normal colon tissue array with colon cancer tissue as control |
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CO727 | TissueArray | each | EUR 270 |
Description: Normal colon tissue array with colon cancer tissue as control, including TNM, clinical stage and pathology grade, 24 cases/ 72 cores |
Bovine anti-Granulocyte-Macrophage Colony Stimulating Factor antibody(GM-CSF Ab)ELISA Kit |
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QY-E60012 | Qayee Biotechnology | 96T | EUR 511.2 |
cDNA - Lupus: Heart |
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C1236122Lup | Biochain | 40 reactions | EUR 737 |
cDNA - Lupus: Liver |
|||
C1236149Lup | Biochain | 40 reactions | EUR 737 |
cDNA - Asthma: Lung |
|||
C1236152Ld-1 | Biochain | 40 reactions | EUR 899 |
Bovine COL1a2 ELISA Kit |
|||
EBC0329 | Abclonal | 96Tests | EUR 625.2 |
Bovine COL2a1 ELISA Kit |
|||
EBC0330 | Abclonal | 96Tests | EUR 625.2 |
Bovine COL3a1 ELISA Kit |
|||
EBC0331 | Abclonal | 96Tests | EUR 625.2 |
Bovine COL6a1 ELISA Kit |
|||
EBC0332 | Abclonal | 96Tests | EUR 625.2 |
Bovine COL8a1 ELISA Kit |
|||
EBC0334 | Abclonal | 96Tests | EUR 625.2 |
Bovine COL9a1 ELISA Kit |
|||
EBC0337 | Abclonal | 96Tests | EUR 625.2 |
cDNA - Lupus: Kidney |
|||
C1236142Lup | Biochain | 40 reactions | EUR 737 |
cDNA - Lupus: Spleen |
|||
C1236246Lup | Biochain | 40 reactions | EUR 737 |
Evo? cDNA Supermix |
|||
M1168-100 | Biovision | each | EUR 457.2 |
Evo? cDNA Supermix |
|||
M1168-25 | Biovision | each | EUR 320.4 |
Bovine COL4A3BP ELISA KIT |
|||
ELI-49322b | Lifescience Market | 96 Tests | EUR 1113.6 |
cDNA - Lupus: stomach |
|||
C1236248Lup | Biochain | 40 reactions | EUR 737 |
Novo? cDNA Supermix |
|||
M1169-100 | Biovision | each | EUR 529.2 |
Novo? cDNA Supermix |
|||
M1169-25 | Biovision | each | EUR 346.8 |
Colon cancer tissue with matched adjacent normal colon tissue array |
|||
BC05118d | TissueArray | each | EUR 306 |
Description: Colon cancer tissue with matched adjacent normal colon tissue array, including TNM, clinical stage and pathology grade, 50 cases/100 cores |
Colon cancer tissue array with matched adjacent normal colon tissue |
|||
CO801a | TissueArray | each | EUR 270 |
Description: Colon cancer tissue array with matched adjacent normal colon tissue, including TNM, clinical stage and pathology grade, 40 cases/ 80 cores, replced by CO801b |
cDNA - Emphysema: Lung |
|||
C1236152Ld-3 | Biochain | 40 reactions | EUR 899 |
cDNA - Pneumonia: Lung |
|||
C1236152Ld-4 | Biochain | 40 reactions | EUR 899 |
cDNA - Lupus: Pancreas |
|||
C1236188Lup | Biochain | 40 reactions | EUR 737 |
Colon cancer with matched colon tissue array (most in grade II) |
|||
CO703a | TissueArray | each | EUR 198 |
Description: Colon cancer with matched colon tissue array (most in grade II), including pathology grade, TNM and clinical stage, 35 cases/70 cores |
Bovine Collagen Purified |
|||
IBOCLG10GM | Innovative research | each | EUR 334 |
Description: Bovine Collagen Purified |
Bovine Collagen Purified |
|||
IBOCLG1GM | Innovative research | each | EUR 100 |
Description: Bovine Collagen Purified |
Bovine Collagen Purified |
|||
IBOCLG5GM | Innovative research | each | EUR 196 |
Description: Bovine Collagen Purified |
cDNA - Bronchitis: Lung |
|||
C1236152Ld-2 | Biochain | 40 reactions | EUR 899 |
First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb) |
|||
20-abx09801620ulSystems | Abbexa |
|
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First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb) |
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20-abx09802120ulSystems | Abbexa |
|
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Bovine ColIV ELISA Kit |
|||
EBC0787 | Abclonal | 96Tests | EUR 625.2 |
Total RNA - Lupus: Colon |
|||
R1236090Lup-50 | Biochain | 50 ug | EUR 421.2 |
Progressive colon adenocarcinoma |
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CO1506 | TissueArray | each | EUR 258 |
Description: Progressive colon adenocarcinoma, matched cancer adjacent with normal adjacent tissue array, including pathology grade, TNM /Stage ( AJCC 8th version) and MMR (MLH1/PMS2/MSH2/MSH6) IHC results, 50 cases/150cores (core size 1.0mm),Recent Year Collection |
Type 1 Bovine Collagen |
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TBC320 | Neuromics | 20ml @ 3mg/ml | EUR 418.8 |
cDNA - hypertension: Vein |
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C1236020Hd-2 | Biochain | 40 reactions | EUR 729 |
Bovine COLXVII ELISA Kit |
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EBC0335 | Abclonal | 96Tests | EUR 625.2 |
Bovine Colicin ELISA Kit |
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EBC0793 | Abclonal | 96Tests | EUR 625.2 |
Bovine Colicin ELISA Kit |
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NSL0108Bo | Sunlong | 96 T | EUR 528 |
cDNA Synthesis SuperMix |
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20-abx09801420ulSystems | Abbexa |
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cDNA - hypertension: Heart |
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C1236122Hd-2 | Biochain | 40 reactions | EUR 729 |
Rat Colon Tissue Lysate |
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IRTCLNTL100UG | Innovative research | each | EUR 245 |
Description: Rat Colon Tissue Lysate |
Evo™ cDNA Kit |
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M1164-100 | Biovision | each | Ask for price |
Evo™ cDNA Kit |
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M1164-25 | Biovision | each | Ask for price |
cDNA - hypertension: Artery |
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C1236013Hd-2 | Biochain | 40 reactions | EUR 729 |
cDNA - hypertension: Kidney |
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C1236142Hd-2 | Biochain | 40 reactions | EUR 729 |
Human Colon Tumor lystae |
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HTL-1327 | Alpha Diagnostics | 100ug | EUR 343.2 |
Bovine Col I ELISA Kit |
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EBC0788 | Abclonal | 96Tests | EUR 625.2 |
Colon carcinoma tissue array with adjacent normal colon tissue as control |
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CO242b | TissueArray | each | EUR 66 |
Description: Colon carcinoma tissue array with adjacent normal colon tissue as control, including TNM, clinical stage and pathology grade, 12 cases/24 cores, replacing CO242a |
Colon Tissue Lysate (Tumor) |
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1716-01 | ProSci | 0.1 mg | EUR 336.3 |
Description: Colon tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Colon Tissue Lysate (Tumor) |
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1716-02 | ProSci | 0.1 mg | EUR 336.3 |
Description: Colon tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Colon Tissue Lysate (Tumor) |
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1716-03 | ProSci | 0.1 mg | EUR 336.3 |
Description: Colon tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Colon Tissue Lysate (Tumor) |
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1716-04 | ProSci | 0.1 mg | EUR 336.3 |
Description: Colon tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Colon cancer tissue array |
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CRM1503 | TissueArray | each | EUR 420 |
Description: Colon cancer tissue array, 150 cases/150 cores |
Colon cancer tissue array |
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CRM1504 | TissueArray | each | EUR 420 |
Description: Colon cancer tissue array, 150 cases/150 cores |
Colon cancer tissue array |
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CRM1506 | TissueArray | each | EUR 420 |
Description: Colon cancer tissue array, 150 cases/150 cores |
Colon cancer tissue array |
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CRM1507 | TissueArray | each | EUR 420 |
Description: Colon cancer tissue array, 150 cases/150 cores |
Colon cancer tissue array |
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CO1502 | TissueArray | each | EUR 258 |
Description: Colon cancer tissue array, including TNM, clinical stage and pathology grade, 75 cases/150 cores |
Colon cancer tissue array |
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CO1503 | TissueArray | each | EUR 258 |
Description: Colon cancer tissue array, including TNM, clinical stage and pathology grade, 75 cases/150 cores |
Colon cancer tissue array |
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CO244 | TissueArray | each | EUR 270 |
Description: Colon cancer tissue array, with normal tissue, including TNM and pathology grade, 2 serial sections, 24 cases/48 cores |
Colon cancer tissue array |
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CO485 | TissueArray | each | EUR 270 |
Description: Colon cancer tissue array, including TNM, clinical stage and pathology grade, 48 cases/48 cores |
Colon cancer tissue array |
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CO486a | TissueArray | each | EUR 414 |
Description: Colon cancer tissue array, including pathology grade, TNM and clinical stage, 2 serial sections, 48 cases/96 cores, replacing CO486 |
Colon cancer tissue array |
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CO641 | TissueArray | each | EUR 258 |
Description: Colon cancer tissue array, including TNM, stage and pathology grade, 64 cases/ 64 cores |
Colon cancer tissue array |
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CO804b | TissueArray | each | EUR 270 |
Description: Colon cancer tissue array, including pathology grade, TNM and clinical stage, 80 cases/80 cores, replcing CO804a |
Colon cancer tissue array |
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CO901a | TissueArray | each | EUR 306 |
Description: Colon cancer tissue array, with adjacent normal colon tissue, including TNM, clinical stage and pathology grade, 50 cases/90 cores, replacing CO901 |
Colon cancer tissue array |
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CO961 | TissueArray | each | EUR 258 |
Description: Colon cancer tissue array, including TNM, clinical stage and pathology grade, 48 cases/96 cores |
Colon cancer tissue array |
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CO962 | TissueArray | each | EUR 258 |
Description: Colon cancer tissue array, including pathology grade, TNM/Stage, 48 cases/96 cores |
Total Protein - Lupus: Colon |
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P1236090Lup | Biochain | 1 mg | EUR 486 |
Human Colon Tissue Lysate |
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IHUCLNTL100UG | Innovative research | each | EUR 361 |
Description: Human Colon Tissue Lysate |
Mouse Colon Tissue Lysate |
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IMSCLNTL100UG | Innovative research | each | EUR 245 |
Description: Mouse Colon Tissue Lysate |
Bovine Col III ELISA Kit |
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EBC0792 | Abclonal | 96Tests | EUR 625.2 |
Colon Tissue Lysate (Normal) |
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1715-01 | ProSci | 0.1 mg | EUR 260.7 |
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Colon Tissue Lysate (Normal) |
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1715-02 | ProSci | 0.1 mg | EUR 260.7 |
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Colon Tissue Lysate (Normal) |
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1715-03 | ProSci | 0.1 mg | EUR 260.7 |
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Colon Tissue Lysate (Normal) |
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1715-04 | ProSci | 0.1 mg | EUR 260.7 |
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Colon Tissue Lysate (Normal) |
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1715-05 | ProSci | 0.1 mg | EUR 260.7 |
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Colon Tissue Lysate (Normal) |
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1715-06 | ProSci | 0.1 mg | EUR 260.7 |
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Colon Tissue Lysate (Normal) |
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1715-07 | ProSci | 0.1 mg | EUR 260.7 |
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Comparative Analysis of Two Automated Multiplex RT-PCR Assessments for Fast Detection of Influenza and Respiratory Syncytial Viruses
Background: Fast and correct prognosis of influenza virus (Flu) and respiratory syncytial virus (RSV) is vital for managing each the affected person and laboratory. We in contrast the cobas Influenza A/B & RSV assay (cobas Liat) with the Simplexa Flu A/B & RSV assay (Simplexa) to guage which check methodology is extra advantageous contemplating the sources of the laboratory and outcomes of check efficiency.
Strategies: A complete of 236 respiratory specimens from sufferers referred for respiratory virus testing had been retrospectively evaluated; 53 specimens examined optimistic for every of Flu A, Flu B, and RSV, and 77 specimens examined unfavorable based mostly on the outcomes of the reference methodology, i.e., the Seegene Allplex Respiratory Panel half/3 (Seegene, Seoul, Korea). The turnaround time (TAT) was 20 minutes per specimen for cobas Liat and 78 minutes per eight speci-mens for Simplexa. The overall hands-on time was round one minute per specimen for each assessments. The specimen quantity required for testing was 200 µL for cobas Liat and 50 µL for Simplexa. Seegene Allplex Respiratory Panel half/Three was used because the reference methodology.
Outcomes: The variety of invalid outcomes was 1 (0.4%) for cobas Liat and 10 (4.2%) for Simplexa (p < 0.05). All outcomes had been according to these of the reference methodology in cobas Liat. The sensitivity and specificity for Flu A, Flu B, and RSVA had been 100% with Simplexa. Nevertheless, the sensitivity for RSVB was 80.0% with Simplexa, which was a statistically vital distinction with the discovering for cobas Liat (p < 0.05). Comparability of the cycle threshold (Ct) values of RSV for Simplexa with the reference methodology confirmed correlation as steady variables (p < 0.001) with a better propensity for acquiring Ct values with Simplexa, the exception being the six false unfavorable outcomes; their Ct values had been greater than 30 within the reference methodology.
Conclusions: Cobas Liat confirmed correct efficiency with a fast TAT and an excellent workflow effectivity. Cobas Liat is extra environment friendly than Simplexa as a point-of-care check for the detection of RSV.
Disagreement between PCR and serological prognosis of Trypanosoma cruzi an infection in blood donors from a Colombian endemic area
Introduction: Chagas’ illness is the main reason behind infectious myocarditis worldwide. This an infection brought on by Trypanosoma cruzi is often life-long and asymptomatic; nonetheless, the third a part of contaminated folks can develop extreme and even deadly cardiomyopathy. Because the parasitemia within the persistent section is each low-grade and intermittent, T. cruzi an infection is principally detected by serology, though this methodology has sensitivity and specificity limitations.
Goal: To find out the extent of settlement between serologic and molecular assessments in 658 voluntary blood donors from six provinces within the Colombian division of Santander.
Supplies and strategies: We evaluated an array of diagnostic applied sciences by cross-section sampling performing a serological double diagnostic check for T. cruzi antibody detection (Chagas III ELISA™, BiosChile Group, and ARCHITECT Chagas CMIA™, Abbott), and DNA detection by polymerase chain response (PCR). We collected the demographic, medical, and epidemiological data of contributors. The pattern measurement was calculated utilizing Epidat™ and the statistical evaluation was finished with Stata 12.1™.
Outcomes: PCR was six occasions extra delicate in detecting T. cruzi an infection than ELISA/CMIA with prevalence values of 1.8% (12/658) and 0.3% (2/658), respectively, and kappa=0.28 (95%CI: -0.03 – 0.59). In distinction, serology confirmed a sensitivity of 16.7% (95%CI: 2.09 – 48.4) and a specificity of 100% (95%CI: 99.4 – 100). All seropositive samples had been discovered to be optimistic by PCR.
Conclusions: The implementation of PCR as a complementary methodology for screening donors might scale back the likelihood of false unfavorable and the resultant danger of transfusional-transmission of Chagas’ illness, particularly in endemic areas.
Simultaneous screening of the FRAXA and FRAXE loci for fast detection of FMR1 CGG and/or AFF2 CCG repeat expansions by triplet-primed PCR
Reasonable to hyper growth of trinucleotide repeats on the FRAXA and FRAXE fragile websites, with or with out concomitant hypermethylation are related to mental incapacity and different situations. Not like molecular prognosis of FMR1 CGG repeat expansions in FRAXA, present detection of AFF2 CCG repeat expansions in FRAXE depends on low-throughput and inefficient strategies combining Southern blot and PCR. A novel triplet-primed PCR assay was developed to concurrently display for trinucleotide repeat expansions on the FRAXA and FRAXE fragile websites, and validated utilizing archived medical samples of identified FMR1 and AFF2 genotypes.
Sequencing of inhabitants samples and FRAXE-affected samples was carried out to guage variation of the AFF2 CCG repeat construction. The duplex assay precisely recognized expansions on the FMR1 and AFF2 trinucleotide repeat loci. Sanger sequencing of the AFF2 CCG repeat revealed that the SNP variant rs868914124(C), which successfully provides two CCG repeats at the 5′ finish, is enriched within the Malay inhabitants and with brief repeats (<11 CCGs), and was current in all six expanded AFF2 alleles of this research. All expanded AFF2 alleles contained a number of non-CCG interruptions in the direction of the 5′ finish of the repeat. We’ve developed a delicate, sturdy and fast assay to concurrently detect growth mutations on the FMR1 and AFF2 trinucleotide repeat loci, simplifying the screening of FRAXA and FRAXE related issues.