CtNorm: Real time PCR cycle of threshold (Ct) normalization algorithm

CtNorm: Actual time PCR cycle of threshold (Ct) normalization algorithm

In relative quantification with Actual Time PCR (qRT-PCR,), correct evaluation requires equal amplification effectivity for each genes (Gene of curiosity and reference gene) and equal threshold values for all of the samples. On this quantification technique the expression stage in handled samples shall be calculated compared to the management group. We carried out the current examine to design an algorithm for changing the info obtained from totally different runs containing similar customary samples into one run with the identical amplification effectivity and threshold worth.
For this goal, two formulation have been designed; one to transform the amplification effectivity of the every run to 100%, and the opposite one for changing knowledge from totally different runs into one run. Using these two formulation, an algorithm was developed and named CtNorm. The web model of CtNorm algorithm is on the market at http://ctnorm.sums.ac.ir/. We used qRT-PCR method to validate the accuracy of the designed algorithm for the normalization of 4 totally different human inside management genes.
Normalizing the Ct values obtained from separate runs with the CtNorm algorithm has eradicated the variations and the common of the Ct values has change into much like the situation during which all of the samples have been amplified in a single run. The CtNorm algorithm could possibly be utilized for equalizing the Ct values of a number of qRT-PCR runs with the identical customary samples. The algorithm has additionally the flexibility to transform the amplification effectivity to 100% which is helpful in absolute and relative quantification.
russelldurrett
russelldurrett

cDNA from Monkey (Cynomolgus) Normal Tissue: Uterus: Cervix

C1534275-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Uterus: Corpus

C1534276 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Uterus: Fundus

C1534278 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human Catalase (CAT) ELISA Kit

DLR-CAT-Hu-48T 48T
EUR 441
Description: A sandwich quantitative ELISA assay kit for detection of Human Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Catalase (CAT) ELISA Kit

DLR-CAT-Hu-96T 96T
EUR 570
Description: A sandwich quantitative ELISA assay kit for detection of Human Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Catalase (CAT) ELISA Kit

DLR-CAT-Mu-48T 48T
EUR 527
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Catalase (CAT) ELISA Kit

DLR-CAT-Mu-96T 96T
EUR 688
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Catalase (CAT) ELISA Kit

DLR-CAT-Ra-48T 48T
EUR 549
Description: A sandwich quantitative ELISA assay kit for detection of Rat Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Catalase (CAT) ELISA Kit

DLR-CAT-Ra-96T 96T
EUR 718
Description: A sandwich quantitative ELISA assay kit for detection of Rat Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Catalase (CAT) ELISA Kit

RD-CAT-Hu-48Tests 48 Tests
EUR 436

Human Catalase (CAT) ELISA Kit

RD-CAT-Hu-96Tests 96 Tests
EUR 601

Mouse Catalase (CAT) ELISA Kit

RD-CAT-Mu-48Tests 48 Tests
EUR 533

Mouse Catalase (CAT) ELISA Kit

RD-CAT-Mu-96Tests 96 Tests
EUR 740

Rat Catalase (CAT) ELISA Kit

RD-CAT-Ra-48Tests 48 Tests
EUR 557

Rat Catalase (CAT) ELISA Kit

RD-CAT-Ra-96Tests 96 Tests
EUR 775

Human Catalase (CAT) ELISA Kit

RDR-CAT-Hu-48Tests 48 Tests
EUR 455

Human Catalase (CAT) ELISA Kit

RDR-CAT-Hu-96Tests 96 Tests
EUR 629

Mouse Catalase (CAT) ELISA Kit

RDR-CAT-Mu-48Tests 48 Tests
EUR 557

Mouse Catalase (CAT) ELISA Kit

RDR-CAT-Mu-96Tests 96 Tests
EUR 774

Rat Catalase (CAT) ELISA Kit

RDR-CAT-Ra-48Tests 48 Tests
EUR 583

Rat Catalase (CAT) ELISA Kit

RDR-CAT-Ra-96Tests 96 Tests
EUR 811

Uterus Lysate

1317 0.1 mg
EUR 191
Description: Uterus tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Uterus Tumor Lysate

1384 0.1 mg
EUR 254
Description: Uterus tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Fetal Uterus Lysate

XBL-10431 0.1 mg
EUR 527
Description: Fetal human uterus tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human uterus tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the uterus tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The uterus tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Uterus Membrane Lysate

XBL-11023 0.1 mg
EUR 516.5
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Bovine UTERUS 5 EA*

57153-2 5 EA
EUR 204.2

Human Uterus Tumor lysate

HTL-1384 1 mg
EUR 773

Uterus Tissue Slide (Normal)

11-401-10um 10 um
EUR 201.5

Uterus Tissue Slide (Normal)

11-401-4um 4 um
EUR 180.5

Uterus Tissue Slide (Benign)

11-402-10um 10 um
EUR 201.5

Uterus Tissue Slide (Benign)

11-402-4um 4 um
EUR 180.5

Uterus Tissue Slide (Adenocarcinoma)

11-404-10um 10 um
EUR 201.5

Uterus Tissue Slide (Adenocarcinoma)

11-404-4um 4 um
EUR 180.5

Uterus Tissue Slide (Abnormal)

11-419-10um 10 um
EUR 201.5

Uterus Tissue Slide (Abnormal)

11-419-4um 4 um
EUR 180.5

Uterus Tissue Slide (Adenomyoma)

11-432-10um 10 um
EUR 201.5

Uterus Tissue Slide (Adenomyoma)

11-432-4um 4 um
EUR 180.5

Uterus Membrane Tumor Lysate

XBL-11031 0.1 mg
EUR 626.75
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Uterus-Corpus Membrane Lysate

XBL-11037 0.1 mg
EUR 516.5
Description: Human uterus-corpus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-corpus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-corpus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-corpus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Uterus-Fundus Membrane Lysate

XBL-11040 0.1 mg
EUR 516.5
Description: Human uterus-fundus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-fundus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-fundus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-fundus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Total RNA from Human Adult Normal Tissue: Uterus: Cervix of uterus

R1234275-50 50 ug
EUR 178
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Adult Normal Tissue: Uterus: Corpus of Uterus

R1234276-10 10 ug
EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

CAT/ Rat CAT ELISA Kit

ELA-E0242r 96 Tests
EUR 886

Rabbit UTERUS Y 25 EA*

41253-2 25 EA
EUR 318.39

Total RNA from Lupus: Uterus

R1236274Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Uterus Tissue Slide (Endometrioid Adenocarcinoma)

11-405-10um 10 um
EUR 201.5

Uterus Tissue Slide (Endometrioid Adenocarcinoma)

11-405-4um 4 um
EUR 180.5

CAT Antibody

36312-100ul 100ul
EUR 252

CAT siRNA

20-abx900807
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

CAT siRNA

20-abx910366
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

CAT siRNA

20-abx910367
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

CAT Antibody

1-CSB-PA070043
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/20000

CAT Antibody

1-CSB-PA599838
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:50-1:200

CAT Antibody

1-CSB-PA942303
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:50-1:200

CAT Antibody

1-CSB-PA004980
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against CAT. Recognizes CAT from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000

CAT Antibody

1-CSB-PA004563GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

pCas9- CAT

PVT11001 2 ug
EUR 301

pOTB7-CAT

PVT18217 2 ug
EUR 231

CAT antibody

10R-3584 100 ul
EUR 726
Description: Mouse monoclonal CAT antibody

CAT antibody

10R-3585 100 ul
EUR 691
Description: Mouse monoclonal CAT antibody

CAT antibody

70R-16187 50 ul
EUR 435
Description: Rabbit polyclonal CAT antibody

CAT antibody

20C-CR1111RP 500 ul
EUR 262
Description: Rabbit polyclonal CAT antibody

Cat IgM

31C-CH0213 1 mg
EUR 392
Description: Purified Cat IgM

Total RNA from Human Adult Normal Tissue 5 Donor Pool: Uterus: Cervix of uterus

R1234275-P 50 ug
EUR 328
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Novo? cDNA Kit

M1165-100
EUR 354

Novo? cDNA Kit

M1165-25
EUR 267

Evo? cDNA Supermix

M1168-100
EUR 381

Evo? cDNA Supermix

M1168-25
EUR 267

Novo? cDNA Supermix

M1169-100
EUR 441

Novo? cDNA Supermix

M1169-25
EUR 289

Frozen Tissue Section - Human Tumor: Uterus

T1235274 5 slides
EUR 338
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Uterus Tissue Slide ( Papillary serous Adenocarcinoma)

11-407-10um 10 um
EUR 201.5

Uterus Tissue Slide ( Papillary serous Adenocarcinoma)

11-407-4um 4 um
EUR 180.5

Uterus Tissue Slide (Spindle Cell Tumor)

11-418-10um 10 um
EUR 201.5

Uterus Tissue Slide (Spindle Cell Tumor)

11-418-4um 4 um
EUR 180.5

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

PKA CAT Antibody

abx147539-100ug 100 ug
EUR 439

Catalase (CAT) Antibody

20-abx132110
  • EUR 272.00
  • EUR 592.00
  • EUR 314.00
  • EUR 154.00
  • EUR 230.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Catalase (CAT) Antibody

20-abx132122
  • EUR 300.00
  • EUR 718.00
  • EUR 384.00
  • EUR 154.00
  • EUR 244.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Catalase (CAT) Antibody

20-abx125619
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Catalase (CAT) Antibody

20-abx125620
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Catalase (CAT) Antibody

20-abx111432
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

CAT Rabbit pAb

A11777-100ul 100 ul
EUR 308

CAT Rabbit pAb

A11777-200ul 200 ul
EUR 459

CAT Rabbit pAb

A11777-20ul 20 ul
EUR 183

CAT Rabbit pAb

A11777-50ul 50 ul
EUR 223

Enchancment of pneumococcal pneumonia prognosis utilizing quantitative real-time PCR concentrating on lytA in grownup sufferers: A potential cohort examine

Aims: The intention of the examine was to evaluate the efficiency of real-time polymerase chain response (PCR) concentrating on the lytA gene (rtPCR-lytA) in plasma, urine and nasopharyngeal (NP) samples for the prognosis of pneumococcal community-acquired pneumonia (P-CAP).
Strategies: Potential observational examine together with all consecutive grownup CAP sufferers from November 2015 to Could 2017. P-CAP was outlined if pneumococcus was recognized utilizing typical strategies (CM) and/or a optimistic rtPCR-lytA was detected in blood, urine or NP samples (NP cut-off ≥8000 copies/mL). Diagnostic efficiency of every check was calculated.
Outcomes: 133 CAP sufferers have been included. Of those, P-CAP was identified in 62 circumstances (46.6%). The proportion of P-CAP identified by rtPCR-lytA strategies was considerably larger than by CM (87.1% vs 59.7%, p=0.005). RtPCR-lytA recognized S. pneumoniae in 25 sufferers (40.3% of all P-CAP circumstances) whose prognosis would have been missed by CM. NP-rtPCR-lytA allowed diagnosing 62.3% of P-CAP. A nasopharyngeal colonisation density ≥2351 copies/mL predicted P-CAP prognosis (AUC=0.82, sensitivity=83.3%, specificity=80.9%). There was a optimistic correlation between growing bacterial load in blood and CURB65 (Spearman correlation coefficient r=0.4, p=0.001), PSI (r=0.3, p=0.02), and time to scientific stability (r=0.33, p=0.01). Median bacterial load in blood was larger in P-CAP sufferers with bacteraemia (0.65×103 vs 0x103 copies/mL, p=0.002), ICU admission (0.68×103 vs 0x103 copies/mL, p=0.04) or mechanical air flow (7.45×103 vs 0x103 copies/mL, p=0.04).
Conclusions: The usage of rtPCR-lytA strategies considerably elevated the prognosis of P-CAP as in comparison with CM. Nasopharyngeal swabs rtPCR-lytA detection, with an correct cut-off worth, was essentially the most promising amongst molecular strategies for the prognosis of P-CAP.

Epidemiology and scientific options of intestinal protozoan infections detected by Actual-Time PCR in non-native kids inside an Italian tertiary care kids’s hospital: a cross-sectional examine

Background: Enteric parasite infections are underestimated because of the restricted sensitivity and specificity of microscopy, which stays the diagnostic gold customary in routine scientific apply. This could possibly be a serious drawback in high-income nations, the place the burden of parasitic illnesses is low. Lately, Multiplex Actual-Time polymerase chain response (RT-PCR) primarily based strategies have been applied. Due to this fact, the intention of this examine was to judge the prevalence of 4 enteric protozoan species detected by RT-PCR in non-native kids in Italy, and to explain their scientific traits.
Strategies: Adopted and immigrant kids, evaluated for migration well being evaluation between 2017 and 2020 in a tertiary care kids’s hospital in Italy, have been enrolled. Molecular evaluation for Giardia lamblia, Dientamoeba fragilis, Blastocystis hominis, and Entamoeba histolytica, was carried out by in-house RT-PCR.
Outcomes: Total, 209 kids have been enrolled and 70% of them resulted optimistic by RT-PCR for no less than one enteric parasite. B. hominis (47.8%) was essentially the most generally recognized protozoa, adopted by D. fragilis (44.5%). Co-infections with a number of pathogens have been detected in 35.4% of the samples. Nearly 80% of parasite-positive kids have been asymptomatic and the most typical symptom was flatulence (60.7% of symptomatic kids). Eosinophils have been considerably elevated in RT-PCR optimistic kids in comparison with the detrimental ones and youngsters with D. fragilis offered the very best eosinophils rely.
Conclusions: The In-house Multiplex RT-PCR assay gives a sound molecular detection system for chosen enteric parasites. This novel and correct diagnostic technique might help in growing the detection price of parasite an infection, particularly in high-risk inhabitants.

Novel common primer-pentaplex PCR assay primarily based on chimeric primers for simultaneous detection of 5 widespread pig viruses related to diarrhea

Viral pathogens related to diarrhea in pigs embody porcine circovirus 2 (PCV2), porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine rotavirus A (RVA) and C (RVC) amongst others. On this examine, a novel common primer-based pentaplex PCR (UP-M-PCR) assay was developed for simultaneous detection and differentiation of those 5 viruses.
The assay makes use of a short-cycle multiplex amplification by chimeric primers (CP), that are virus particular, with a tail added on the 5′ finish of the common primer (UP), adopted by common amplification utilizing UPs and a common cycle amplification. 5 common primers with CPs (UP1-5) have been designed and evaluated in an UP-based single PCR (UP-S-PCR). All 5 UPs have been discovered to work effectively and UP2 exhibited the most effective efficiency.
After system optimizations, the analytical sensitivity of the UP-M-PCR, utilizing plasmids containing the particular viral goal fragments, was 5 copies/response for every of the 5 viruses regardless of presence of a single or a number of viruses within the template. No cross-reaction was noticed with different non-target viruses. When 273 fecal samples from clinically wholesome pigs have been examined, the assay sensitivity was 90.9-100%, the specificity was 98.0-100%, and the settlement price with the UP-S-PCR was 98.5-99.6% with a Kappa worth being 0.95-0.98. In abstract, the UP-M-PCR developed here’s a fast and extremely delicate and particular detection technique that can be utilized to reveal combined infections in pigs with diarrhea.

SLC52A1 Antibody

1-CSB-PA030173
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against SLC52A1. Recognizes SLC52A1 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/10000

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

SLC52A1 Polyclonal Antibody

A67622 100 µg
EUR 570.55
Description: fast delivery possible

SLC52A1 Antibody (HRP)

20-abx311449
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

SLC52A1 Antibody (FITC)

20-abx311450
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

SLC52A1 Antibody (Biotin)

20-abx311451
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

SLC52A1 Conjugated Antibody

C37605 100ul
EUR 397

SLC52A1 siRNA

20-abx934068
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

SLC52A1 Antibody, HRP conjugated

1-CSB-PA878904LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against SLC52A1. Recognizes SLC52A1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

SLC52A1 Antibody, FITC conjugated

1-CSB-PA878904LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against SLC52A1. Recognizes SLC52A1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

SLC52A1 Antibody, Biotin conjugated

1-CSB-PA878904LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against SLC52A1. Recognizes SLC52A1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

SLC52A1 Polyclonal Antibody, HRP Conjugated

A67623 100 µg
EUR 570.55
Description: reagents widely cited

SLC52A1 Polyclonal Antibody, FITC Conjugated

A67624 100 µg
EUR 570.55
Description: Ask the seller for details

SLC52A1 Polyclonal Antibody, Biotin Conjugated

A67625 100 µg
EUR 570.55
Description: The best epigenetics products

Human SLC52A1 shRNA Plasmid

20-abx960440
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

SLC52A1 Recombinant Protein (Human)

RP097485 100 ug Ask for price

SLC52A1 ORF Vector (Human) (pORF)

ORF032496 1.0 ug DNA Ask for price

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Solute Carrier Family 52 Member 1 (SLC52A1) Antibody

20-abx302068
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Solute Carrier Family 52 Member 1 (SLC52A1) Antibody

20-abx211053
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Solute Carrier Family 52 Member 1 (SLC52A1) Antibody

20-abx211054
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Solute Carrier Family 52 Member 1 (SLC52A1) Antibody

20-abx324146
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

SLC52A1 Protein Vector (Human) (pPB-C-His)

PV129982 500 ng Ask for price

SLC52A1 Protein Vector (Human) (pPB-N-His)

PV129983 500 ng Ask for price

SLC52A1 Protein Vector (Human) (pPM-C-HA)

PV129984 500 ng Ask for price

SLC52A1 Protein Vector (Human) (pPM-C-His)

PV129985 500 ng Ask for price

Anti-Anti-SEPT6 antibody antibody

STJ11100949 100 µl
EUR 277
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.

Anti-Anti-SEPT9 Antibody antibody

STJ111369 100 µl
EUR 277
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.

Anti-Anti-SEPT11 Antibody antibody

STJ111530 100 µl
EUR 277

Anti-Anti-SEPT4 Antibody antibody

STJ112276 100 µl
EUR 277
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.

Anti-Anti-MARCH9 Antibody antibody

STJ112609 100 µl
EUR 277

Anti-Anti-SEPT2 Antibody antibody

STJ25475 100 µl
EUR 277

Anti-Anti-SEPT5 Antibody antibody

STJ25477 100 µl
EUR 277
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-SEPT8 Antibody antibody

STJ25479 100 µl
EUR 277
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT2 Antibody antibody

STJ28365 100 µl
EUR 277

Anti-Anti-SEPT7 Antibody antibody

STJ28963 100 µl
EUR 277
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-SEPT7 Antibody antibody

STJ116214 100 µl
EUR 277
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-SEPT8 Antibody antibody

STJ117206 100 µl
EUR 277
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT12 Antibody antibody

STJ117759 100 µl
EUR 277
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-MARCH6 Antibody antibody

STJ118549 100 µl
EUR 277

Anti-Anti-MARCH6 Antibody antibody

STJ118550 100 µl
EUR 277

Anti-Anti-MARCH7 Antibody antibody

STJ118752 100 µl
EUR 277

Anti-Anti-SEPT3 Antibody antibody

STJ118990 100 µl
EUR 277

Anti-Anti-SEPT1 antibody antibody

STJ119580 100 µl
EUR 277
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]

Anti-Anti-SEPT11 Antibody antibody

STJ113941 100 µl
EUR 277

Anti-Anti-SEPT11 Antibody antibody

STJ114081 100 µl
EUR 277

Anti-Anti-SEPT5 Antibody antibody

STJ114819 100 µl
EUR 277
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-MARCH8 Antibody antibody

STJ114828 100 µl
EUR 277

Anti-Anti-DDB1 Antibody

A00333 100uL
EUR 455
Description: Rabbit Polyclonal DDB1 Antibody. Validated in IP and tested in Human, Mouse.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

AEA465Hu-10x96wellstestplate 10x96-wells test plate
EUR 5647.8
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

AEA465Hu-1x48wellstestplate 1x48-wells test plate
EUR 552.76
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

AEA465Hu-1x96wellstestplate 1x96-wells test plate
EUR 746.8
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

AEA465Hu-5x96wellstestplate 5x96-wells test plate
EUR 3060.6
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

4-AEA465Hu
  • EUR 5698.00
  • EUR 3011.00
  • EUR 747.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

ELISA kit for Human Anti-AsAb (Anti-Anti-Sperm Antibody Antibody)

ELK8071 1 plate of 96 wells
EUR 432
Description: A competitive Inhibition ELISA kit for detection of Anti-Anti-Sperm Antibody Antibody from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Anti-MeCP2 Antibody

M00047-1 100ul
EUR 397
Description: Rabbit Polyclonal MeCP2 Antibody. Validated in IHC, WB and tested in Human, Monkey, Mouse, Rat.

Anti-GAPDH Antibody

M00227-4 100ul
EUR 397
Description: Rabbit Polyclonal GAPDH Antibody. Validated in IF, IHC, WB and tested in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat.

Anti-Vimentin Antibody

M00235-4 100ul
EUR 397
Description: Rabbit Polyclonal Vimentin Antibody. Validated in IHC, WB and tested in Bovine, Equine, Human, Mouse, Pig, Rat.

Anti-Vimentin Antibody

M00235-5 100ul
EUR 397
Description: Chicken Polyclonal Vimentin Antibody. Validated in IHC, WB and tested in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat.

Anti-CNP Antibody

M01017-2 100ul
EUR 397
Description: Rabbit Polyclonal CNP Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-CNP Antibody

M01017-3 100ul
EUR 397
Description: Chicken Polyclonal CNP Antibody. Validated in IHC, WB and tested in Equine, Pig.

Anti-GAP43 Antibody

M01868-3 100ul
EUR 397
Description: Rabbit Polyclonal GAP43 Antibody. Validated in IF, IHC, WB and tested in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat.

Anti-GAP43 Antibody

M01868-4 100ul
EUR 397
Description: Chicken Polyclonal GAP43 Antibody. Validated in IF, IHC, WB and tested in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat.

Anti-GPSN2 Antibody

F08289-1 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for GPSN2 Antibody (TECR) detection.tested for WB in Human, Mouse, Rat.

Anti-Calbindin Antibody

M03047-2 100ul
EUR 397
Description: Chicken Polyclonal Calbindin Antibody. Validated in IF, IHC, WB and tested in Bovine, Human, Mouse, Rat.

Anti-Fibrillarin Antibody

M03178-4 100ul
EUR 397
Description: Rabbit Polyclonal Fibrillarin Antibody. Validated in IP, IHC, WB and tested in Human, Mouse, Rat.

Anti-Fibrillarin Antibody

M03178-5 100ul
EUR 397
Description: Chicken Polyclonal Fibrillarin Antibody. Validated in IF, IHC, WB and tested in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat.

Anti-Parvalbumin Antibody

M04041-2 100ul
EUR 397
Description: Chicken Polyclonal Parvalbumin Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

Anti-Calretinin Antibody

M04255-3 100ul
EUR 397
Description: Rabbit Polyclonal Calretinin Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-Calretinin Antibody

M04255-4 100ul
EUR 397
Description: Chicken Polyclonal Calretinin Antibody. Validated in IF, IHC, WB and tested in Bovine, Equine, Human, Mouse, Pig, Rat.

Anti-Laminin Antibody

M04965 100ul
EUR 397
Description: Rabbit Polyclonal Laminin Antibody. Validated in IHC and tested in Human, Mouse, Rat.

Anti-Secretagogin Antibody

M10629-1 100ul
EUR 397
Description: Rabbit Polyclonal Secretagogin Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

Anti-Secretagogin Antibody

M10629-2 100ul
EUR 397
Description: Chicken Polyclonal Secretagogin Antibody. Validated in IF, IHC, WB and tested in Bovine, Human, Mouse, Rat.

Anti-ATF6 Antibody

PA1011 100ug/vial
EUR 334

Anti-ATF6 Antibody

PA1011-1 100ug/vial
EUR 294

Anti-ATP5J Antibody

PA1012 100ug/vial
EUR 294

Anti-Bax Antibody

PA1013 100ug/vial
EUR 294

Anti-Bax Antibody

PA1013-1 100ug/vial
EUR 294

Anti-BDNF Antibody

PA1014 100ug/vial
EUR 294

Anti-CCR5 Antibody

PA1016 100ug/vial
EUR 294

Anti-CCR5 Antibody

PA1016-1 100ug/vial
EUR 294

Anti-CCR5 Antibody

PA1016-2 100ug/vial
EUR 294

Anti-CCR7 Antibody

PA1017 100ug/vial
EUR 294

Anti-CD22 Antibody

PA1018 100ug/vial
EUR 334

Anti-CD22 Antibody

PA1018-1 100ug/vial
EUR 334

Anti-CD40 Antibody

PA1019 100ug/vial
EUR 294

Anti-CD40 Antibody

PA1019-1 100ug/vial
EUR 294

Anti-CD44 Antibody

PA1021 100ug/vial
EUR 334

Anti-CD44 Antibody

PA1021-1 100ug/vial
EUR 334

Anti-CD44 Antibody

PA1021-2 100ug/vial
EUR 334

Anti-GJB2 Antibody

PA1025 100ug/vial
EUR 294

Anti-CXCR2 Antibody

PA1029 100ug/vial
EUR 294

Anti-dUTPase Antibody

PA1030 100ug/vial
EUR 334

Anti-DUT Antibody

PA1030-1 100ug/vial
EUR 334

Leave a Reply

Your email address will not be published.