CtNorm: Actual time PCR cycle of threshold (Ct) normalization algorithm
In relative quantification with Actual Time PCR (qRT-PCR,), correct evaluation requires equal amplification effectivity for each genes (Gene of curiosity and reference gene) and equal threshold values for all of the samples. On this quantification technique the expression stage in handled samples shall be calculated compared to the management group. We carried out the current examine to design an algorithm for changing the info obtained from totally different runs containing similar customary samples into one run with the identical amplification effectivity and threshold worth.
For this goal, two formulation have been designed; one to transform the amplification effectivity of the every run to 100%, and the opposite one for changing knowledge from totally different runs into one run. Using these two formulation, an algorithm was developed and named CtNorm. The web model of CtNorm algorithm is on the market at http://ctnorm.sums.ac.ir/. We used qRT-PCR method to validate the accuracy of the designed algorithm for the normalization of 4 totally different human inside management genes.
Normalizing the Ct values obtained from separate runs with the CtNorm algorithm has eradicated the variations and the common of the Ct values has change into much like the situation during which all of the samples have been amplified in a single run. The CtNorm algorithm could possibly be utilized for equalizing the Ct values of a number of qRT-PCR runs with the identical customary samples. The algorithm has additionally the flexibility to transform the amplification effectivity to 100% which is helpful in absolute and relative quantification.
cDNA from Monkey (Cynomolgus) Normal Tissue: Uterus: Cervix |
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| C1534275-Cy | Biochain | 40 reactions | EUR 540 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Uterus: Corpus |
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| C1534276 | Biochain | 40 reactions | EUR 540 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Uterus: Fundus |
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| C1534278 | Biochain | 40 reactions | EUR 540 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Human Catalase (CAT) ELISA Kit |
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| DLR-CAT-Hu-48T | DL Develop | 48T | EUR 441 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Human Catalase (CAT) ELISA Kit |
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| DLR-CAT-Hu-96T | DL Develop | 96T | EUR 570 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Mouse Catalase (CAT) ELISA Kit |
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| DLR-CAT-Mu-48T | DL Develop | 48T | EUR 527 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Mouse Catalase (CAT) ELISA Kit |
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| DLR-CAT-Mu-96T | DL Develop | 96T | EUR 688 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Rat Catalase (CAT) ELISA Kit |
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| DLR-CAT-Ra-48T | DL Develop | 48T | EUR 549 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Rat Catalase (CAT) ELISA Kit |
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| DLR-CAT-Ra-96T | DL Develop | 96T | EUR 718 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Human Catalase (CAT) ELISA Kit |
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| RD-CAT-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 436 |
Human Catalase (CAT) ELISA Kit |
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| RD-CAT-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 601 |
Mouse Catalase (CAT) ELISA Kit |
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| RD-CAT-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 533 |
Mouse Catalase (CAT) ELISA Kit |
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| RD-CAT-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 740 |
Rat Catalase (CAT) ELISA Kit |
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| RD-CAT-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 557 |
Rat Catalase (CAT) ELISA Kit |
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| RD-CAT-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 775 |
Human Catalase (CAT) ELISA Kit |
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| RDR-CAT-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 455 |
Human Catalase (CAT) ELISA Kit |
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| RDR-CAT-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 629 |
Mouse Catalase (CAT) ELISA Kit |
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| RDR-CAT-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 557 |
Mouse Catalase (CAT) ELISA Kit |
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| RDR-CAT-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 774 |
Rat Catalase (CAT) ELISA Kit |
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| RDR-CAT-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 583 |
Rat Catalase (CAT) ELISA Kit |
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| RDR-CAT-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 811 |
Uterus Lysate |
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| 1317 | ProSci | 0.1 mg | EUR 191 |
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Description: Uterus tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Uterus Tumor Lysate |
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| 1384 | ProSci | 0.1 mg | EUR 254 |
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Description: Uterus tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Fetal Uterus Lysate |
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| XBL-10431 | ProSci | 0.1 mg | EUR 527 |
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Description: Fetal human uterus tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human uterus tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the uterus tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The uterus tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Uterus Membrane Lysate |
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| XBL-11023 | ProSci | 0.1 mg | EUR 516.5 |
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Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
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Bovine UTERUS 5 EA* |
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| 57153-2 | Pel-Freez | 5 EA | EUR 204.2 |
Human Uterus Tumor lysate |
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| HTL-1384 | Alpha Diagnostics | 1 mg | EUR 773 |
Uterus Tissue Slide (Normal) |
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| 11-401-10um | ProSci | 10 um | EUR 201.5 |
Uterus Tissue Slide (Normal) |
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| 11-401-4um | ProSci | 4 um | EUR 180.5 |
Uterus Tissue Slide (Benign) |
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| 11-402-10um | ProSci | 10 um | EUR 201.5 |
Uterus Tissue Slide (Benign) |
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| 11-402-4um | ProSci | 4 um | EUR 180.5 |
Uterus Tissue Slide (Adenocarcinoma) |
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| 11-404-10um | ProSci | 10 um | EUR 201.5 |
Uterus Tissue Slide (Adenocarcinoma) |
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| 11-404-4um | ProSci | 4 um | EUR 180.5 |
Uterus Tissue Slide (Abnormal) |
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| 11-419-10um | ProSci | 10 um | EUR 201.5 |
Uterus Tissue Slide (Abnormal) |
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| 11-419-4um | ProSci | 4 um | EUR 180.5 |
Uterus Tissue Slide (Adenomyoma) |
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| 11-432-10um | ProSci | 10 um | EUR 201.5 |
Uterus Tissue Slide (Adenomyoma) |
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| 11-432-4um | ProSci | 4 um | EUR 180.5 |
Uterus Membrane Tumor Lysate |
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| XBL-11031 | ProSci | 0.1 mg | EUR 626.75 |
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Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
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Uterus-Corpus Membrane Lysate |
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| XBL-11037 | ProSci | 0.1 mg | EUR 516.5 |
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Description: Human uterus-corpus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-corpus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-corpus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-corpus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
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Uterus-Fundus Membrane Lysate |
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| XBL-11040 | ProSci | 0.1 mg | EUR 516.5 |
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Description: Human uterus-fundus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-fundus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-fundus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-fundus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
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Total RNA from Human Adult Normal Tissue: Uterus: Cervix of uterus |
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| R1234275-50 | Biochain | 50 ug | EUR 178 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Total RNA from Human Adult Normal Tissue: Uterus: Corpus of Uterus |
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| R1234276-10 | Biochain | 10 ug | EUR 194 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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CAT/ Rat CAT ELISA Kit |
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| ELA-E0242r | Lifescience Market | 96 Tests | EUR 886 |
Rabbit UTERUS Y 25 EA* |
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| 41253-2 | Pel-Freez | 25 EA | EUR 318.39 |
Total RNA from Lupus: Uterus |
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| R1236274Lup-50 | Biochain | 50 ug | EUR 351 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Uterus Tissue Slide (Endometrioid Adenocarcinoma) |
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| 11-405-10um | ProSci | 10 um | EUR 201.5 |
Uterus Tissue Slide (Endometrioid Adenocarcinoma) |
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| 11-405-4um | ProSci | 4 um | EUR 180.5 |
CAT Antibody |
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| 36312-100ul | SAB | 100ul | EUR 252 |
CAT siRNA |
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| 20-abx900807 | Abbexa |
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CAT siRNA |
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| 20-abx910366 | Abbexa |
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CAT siRNA |
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| 20-abx910367 | Abbexa |
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CAT Antibody |
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| 1-CSB-PA070043 | Cusabio |
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Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/20000 |
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CAT Antibody |
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| 1-CSB-PA599838 | Cusabio |
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Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:50-1:200 |
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CAT Antibody |
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| 1-CSB-PA942303 | Cusabio |
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Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:50-1:200 |
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CAT Antibody |
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| 1-CSB-PA004980 | Cusabio |
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Description: A polyclonal antibody against CAT. Recognizes CAT from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000 |
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CAT Antibody |
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| 1-CSB-PA004563GA01HU | Cusabio |
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Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC |
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pCas9- CAT |
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| PVT11001 | Lifescience Market | 2 ug | EUR 301 |
pOTB7-CAT |
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| PVT18217 | Lifescience Market | 2 ug | EUR 231 |
CAT antibody |
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| 10R-3584 | Fitzgerald | 100 ul | EUR 726 |
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Description: Mouse monoclonal CAT antibody |
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CAT antibody |
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| 10R-3585 | Fitzgerald | 100 ul | EUR 691 |
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Description: Mouse monoclonal CAT antibody |
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CAT antibody |
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| 70R-16187 | Fitzgerald | 50 ul | EUR 435 |
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Description: Rabbit polyclonal CAT antibody |
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CAT antibody |
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| 20C-CR1111RP | Fitzgerald | 500 ul | EUR 262 |
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Description: Rabbit polyclonal CAT antibody |
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Cat IgM |
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| 31C-CH0213 | Fitzgerald | 1 mg | EUR 392 |
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Description: Purified Cat IgM |
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Total RNA from Human Adult Normal Tissue 5 Donor Pool: Uterus: Cervix of uterus |
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| R1234275-P | Biochain | 50 ug | EUR 328 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA Synthesis SuperMix |
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| 20-abx09801420ulSystems | Abbexa |
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Novo? cDNA Kit |
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| M1165-100 | Biovision | EUR 354 | |
Novo? cDNA Kit |
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| M1165-25 | Biovision | EUR 267 | |
Evo? cDNA Supermix |
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| M1168-100 | Biovision | EUR 381 | |
Evo? cDNA Supermix |
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| M1168-25 | Biovision | EUR 267 | |
Novo? cDNA Supermix |
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| M1169-100 | Biovision | EUR 441 | |
Novo? cDNA Supermix |
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| M1169-25 | Biovision | EUR 289 | |
Frozen Tissue Section - Human Tumor: Uterus |
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| T1235274 | Biochain | 5 slides | EUR 338 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Uterus Tissue Slide ( Papillary serous Adenocarcinoma) |
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| 11-407-10um | ProSci | 10 um | EUR 201.5 |
Uterus Tissue Slide ( Papillary serous Adenocarcinoma) |
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| 11-407-4um | ProSci | 4 um | EUR 180.5 |
Uterus Tissue Slide (Spindle Cell Tumor) |
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| 11-418-10um | ProSci | 10 um | EUR 201.5 |
Uterus Tissue Slide (Spindle Cell Tumor) |
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| 11-418-4um | ProSci | 4 um | EUR 180.5 |
cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis |
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| C1634310 | Biochain | 40 reactions | EUR 621 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Plant Normal Tissue: cDNA from Plant: Corn |
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| C1634330 | Biochain | 40 reactions | EUR 621 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Plant Normal Tissue: cDNA from Plant: Orange |
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| C1634340 | Biochain | 40 reactions | EUR 621 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Plant Normal Tissue: cDNA from Plant: Potato |
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| C1634350 | Biochain | 40 reactions | EUR 621 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Plant Normal Tissue: cDNA from Plant: Rice |
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| C1634360 | Biochain | 40 reactions | EUR 621 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Plant Normal Tissue: cDNA from Plant: Wheat |
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| C1634390 | Biochain | 40 reactions | EUR 621 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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PKA CAT Antibody |
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| abx147539-100ug | Abbexa | 100 ug | EUR 439 |
Catalase (CAT) Antibody |
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| 20-abx132110 | Abbexa |
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Catalase (CAT) Antibody |
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| 20-abx132122 | Abbexa |
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Catalase (CAT) Antibody |
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| 20-abx125619 | Abbexa |
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Catalase (CAT) Antibody |
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| 20-abx125620 | Abbexa |
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Catalase (CAT) Antibody |
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| 20-abx111432 | Abbexa |
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CAT Rabbit pAb |
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| A11777-100ul | Abclonal | 100 ul | EUR 308 |
CAT Rabbit pAb |
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| A11777-200ul | Abclonal | 200 ul | EUR 459 |
CAT Rabbit pAb |
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| A11777-20ul | Abclonal | 20 ul | EUR 183 |
CAT Rabbit pAb |
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| A11777-50ul | Abclonal | 50 ul | EUR 223 |
Enchancment of pneumococcal pneumonia prognosis utilizing quantitative real-time PCR concentrating on lytA in grownup sufferers: A potential cohort examine
Aims: The intention of the examine was to evaluate the efficiency of real-time polymerase chain response (PCR) concentrating on the lytA gene (rtPCR-lytA) in plasma, urine and nasopharyngeal (NP) samples for the prognosis of pneumococcal community-acquired pneumonia (P-CAP).
Strategies: Potential observational examine together with all consecutive grownup CAP sufferers from November 2015 to Could 2017. P-CAP was outlined if pneumococcus was recognized utilizing typical strategies (CM) and/or a optimistic rtPCR-lytA was detected in blood, urine or NP samples (NP cut-off ≥8000 copies/mL). Diagnostic efficiency of every check was calculated.
Outcomes: 133 CAP sufferers have been included. Of those, P-CAP was identified in 62 circumstances (46.6%). The proportion of P-CAP identified by rtPCR-lytA strategies was considerably larger than by CM (87.1% vs 59.7%, p=0.005). RtPCR-lytA recognized S. pneumoniae in 25 sufferers (40.3% of all P-CAP circumstances) whose prognosis would have been missed by CM. NP-rtPCR-lytA allowed diagnosing 62.3% of P-CAP. A nasopharyngeal colonisation density ≥2351 copies/mL predicted P-CAP prognosis (AUC=0.82, sensitivity=83.3%, specificity=80.9%). There was a optimistic correlation between growing bacterial load in blood and CURB65 (Spearman correlation coefficient r=0.4, p=0.001), PSI (r=0.3, p=0.02), and time to scientific stability (r=0.33, p=0.01). Median bacterial load in blood was larger in P-CAP sufferers with bacteraemia (0.65×103 vs 0x103 copies/mL, p=0.002), ICU admission (0.68×103 vs 0x103 copies/mL, p=0.04) or mechanical air flow (7.45×103 vs 0x103 copies/mL, p=0.04).
Conclusions: The usage of rtPCR-lytA strategies considerably elevated the prognosis of P-CAP as in comparison with CM. Nasopharyngeal swabs rtPCR-lytA detection, with an correct cut-off worth, was essentially the most promising amongst molecular strategies for the prognosis of P-CAP.
Epidemiology and scientific options of intestinal protozoan infections detected by Actual-Time PCR in non-native kids inside an Italian tertiary care kids’s hospital: a cross-sectional examine
Background: Enteric parasite infections are underestimated because of the restricted sensitivity and specificity of microscopy, which stays the diagnostic gold customary in routine scientific apply. This could possibly be a serious drawback in high-income nations, the place the burden of parasitic illnesses is low. Lately, Multiplex Actual-Time polymerase chain response (RT-PCR) primarily based strategies have been applied. Due to this fact, the intention of this examine was to judge the prevalence of 4 enteric protozoan species detected by RT-PCR in non-native kids in Italy, and to explain their scientific traits.
Strategies: Adopted and immigrant kids, evaluated for migration well being evaluation between 2017 and 2020 in a tertiary care kids’s hospital in Italy, have been enrolled. Molecular evaluation for Giardia lamblia, Dientamoeba fragilis, Blastocystis hominis, and Entamoeba histolytica, was carried out by in-house RT-PCR.
Outcomes: Total, 209 kids have been enrolled and 70% of them resulted optimistic by RT-PCR for no less than one enteric parasite. B. hominis (47.8%) was essentially the most generally recognized protozoa, adopted by D. fragilis (44.5%). Co-infections with a number of pathogens have been detected in 35.4% of the samples. Nearly 80% of parasite-positive kids have been asymptomatic and the most typical symptom was flatulence (60.7% of symptomatic kids). Eosinophils have been considerably elevated in RT-PCR optimistic kids in comparison with the detrimental ones and youngsters with D. fragilis offered the very best eosinophils rely.
Conclusions: The In-house Multiplex RT-PCR assay gives a sound molecular detection system for chosen enteric parasites. This novel and correct diagnostic technique might help in growing the detection price of parasite an infection, particularly in high-risk inhabitants.
Novel common primer-pentaplex PCR assay primarily based on chimeric primers for simultaneous detection of 5 widespread pig viruses related to diarrhea
Viral pathogens related to diarrhea in pigs embody porcine circovirus 2 (PCV2), porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine rotavirus A (RVA) and C (RVC) amongst others. On this examine, a novel common primer-based pentaplex PCR (UP-M-PCR) assay was developed for simultaneous detection and differentiation of those 5 viruses.
The assay makes use of a short-cycle multiplex amplification by chimeric primers (CP), that are virus particular, with a tail added on the 5′ finish of the common primer (UP), adopted by common amplification utilizing UPs and a common cycle amplification. 5 common primers with CPs (UP1-5) have been designed and evaluated in an UP-based single PCR (UP-S-PCR). All 5 UPs have been discovered to work effectively and UP2 exhibited the most effective efficiency.
After system optimizations, the analytical sensitivity of the UP-M-PCR, utilizing plasmids containing the particular viral goal fragments, was 5 copies/response for every of the 5 viruses regardless of presence of a single or a number of viruses within the template. No cross-reaction was noticed with different non-target viruses. When 273 fecal samples from clinically wholesome pigs have been examined, the assay sensitivity was 90.9-100%, the specificity was 98.0-100%, and the settlement price with the UP-S-PCR was 98.5-99.6% with a Kappa worth being 0.95-0.98. In abstract, the UP-M-PCR developed here’s a fast and extremely delicate and particular detection technique that can be utilized to reveal combined infections in pigs with diarrhea.
SLC52A1 Antibody |
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| 1-CSB-PA030173 | Cusabio |
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Description: A polyclonal antibody against SLC52A1. Recognizes SLC52A1 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/10000 |
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Rabbit Polyclonal antibody Anti-CRBN |
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| Anti-CRBN | ImmunoStep | 50 µg | EUR 349 |
SLC52A1 Polyclonal Antibody |
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| A67622 | EpiGentek | 100 µg | EUR 570.55 |
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Description: fast delivery possible |
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SLC52A1 Antibody (HRP) |
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| 20-abx311449 | Abbexa |
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SLC52A1 Antibody (FITC) |
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| 20-abx311450 | Abbexa |
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SLC52A1 Antibody (Biotin) |
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| 20-abx311451 | Abbexa |
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SLC52A1 Conjugated Antibody |
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| C37605 | SAB | 100ul | EUR 397 |
SLC52A1 siRNA |
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| 20-abx934068 | Abbexa |
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SLC52A1 Antibody, HRP conjugated |
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| 1-CSB-PA878904LB01HU | Cusabio |
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Description: A polyclonal antibody against SLC52A1. Recognizes SLC52A1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
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SLC52A1 Antibody, FITC conjugated |
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| 1-CSB-PA878904LC01HU | Cusabio |
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Description: A polyclonal antibody against SLC52A1. Recognizes SLC52A1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
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SLC52A1 Antibody, Biotin conjugated |
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| 1-CSB-PA878904LD01HU | Cusabio |
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Description: A polyclonal antibody against SLC52A1. Recognizes SLC52A1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
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SLC52A1 Polyclonal Antibody, HRP Conjugated |
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| A67623 | EpiGentek | 100 µg | EUR 570.55 |
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Description: reagents widely cited |
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SLC52A1 Polyclonal Antibody, FITC Conjugated |
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| A67624 | EpiGentek | 100 µg | EUR 570.55 |
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Description: Ask the seller for details |
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SLC52A1 Polyclonal Antibody, Biotin Conjugated |
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| A67625 | EpiGentek | 100 µg | EUR 570.55 |
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Description: The best epigenetics products |
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Human SLC52A1 shRNA Plasmid |
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| 20-abx960440 | Abbexa |
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SLC52A1 Recombinant Protein (Human) |
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| RP097485 | ABM | 100 ug | Ask for price |
SLC52A1 ORF Vector (Human) (pORF) |
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| ORF032496 | ABM | 1.0 ug DNA | Ask for price |
Polyclonal Goat anti-GST α-form |
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| GST-ANTI-1 | Detroit R&D | 50 uL | EUR 280 |
Polyclonal Goat anti-GST μ-form |
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| GST-ANTI-2 | Detroit R&D | 50 uL | EUR 280 |
Polyclonal Goat anti-GST p-form |
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| GST-ANTI-3 | Detroit R&D | 50 uL | EUR 280 |
Solute Carrier Family 52 Member 1 (SLC52A1) Antibody |
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| 20-abx302068 | Abbexa |
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Solute Carrier Family 52 Member 1 (SLC52A1) Antibody |
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| 20-abx211053 | Abbexa |
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Solute Carrier Family 52 Member 1 (SLC52A1) Antibody |
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| 20-abx211054 | Abbexa |
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Solute Carrier Family 52 Member 1 (SLC52A1) Antibody |
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| 20-abx324146 | Abbexa |
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SLC52A1 Protein Vector (Human) (pPB-C-His) |
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| PV129982 | ABM | 500 ng | Ask for price |
SLC52A1 Protein Vector (Human) (pPB-N-His) |
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| PV129983 | ABM | 500 ng | Ask for price |
SLC52A1 Protein Vector (Human) (pPM-C-HA) |
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| PV129984 | ABM | 500 ng | Ask for price |
SLC52A1 Protein Vector (Human) (pPM-C-His) |
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| PV129985 | ABM | 500 ng | Ask for price |
Anti-Anti-SEPT6 antibody antibody |
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| STJ11100949 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined. |
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Anti-Anti-SEPT9 Antibody antibody |
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| STJ111369 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described. |
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Anti-Anti-SEPT11 Antibody antibody |
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| STJ111530 | St John's Laboratory | 100 µl | EUR 277 |
Anti-Anti-SEPT4 Antibody antibody |
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| STJ112276 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer. |
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Anti-Anti-MARCH9 Antibody antibody |
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| STJ112609 | St John's Laboratory | 100 µl | EUR 277 |
Anti-Anti-SEPT2 Antibody antibody |
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| STJ25475 | St John's Laboratory | 100 µl | EUR 277 |
Anti-Anti-SEPT5 Antibody antibody |
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| STJ25477 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. |
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Anti-Anti-SEPT8 Antibody antibody |
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| STJ25479 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
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Anti-Anti-SEPT2 Antibody antibody |
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| STJ28365 | St John's Laboratory | 100 µl | EUR 277 |
Anti-Anti-SEPT7 Antibody antibody |
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| STJ28963 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. |
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Anti-Anti-SEPT7 Antibody antibody |
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| STJ116214 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. |
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Anti-Anti-SEPT8 Antibody antibody |
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| STJ117206 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
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Anti-Anti-SEPT12 Antibody antibody |
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| STJ117759 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene. |
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Anti-Anti-MARCH6 Antibody antibody |
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| STJ118549 | St John's Laboratory | 100 µl | EUR 277 |
Anti-Anti-MARCH6 Antibody antibody |
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| STJ118550 | St John's Laboratory | 100 µl | EUR 277 |
Anti-Anti-MARCH7 Antibody antibody |
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| STJ118752 | St John's Laboratory | 100 µl | EUR 277 |
Anti-Anti-SEPT3 Antibody antibody |
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| STJ118990 | St John's Laboratory | 100 µl | EUR 277 |
Anti-Anti-SEPT1 antibody antibody |
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| STJ119580 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012] |
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Anti-Anti-SEPT11 Antibody antibody |
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| STJ113941 | St John's Laboratory | 100 µl | EUR 277 |
Anti-Anti-SEPT11 Antibody antibody |
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| STJ114081 | St John's Laboratory | 100 µl | EUR 277 |
Anti-Anti-SEPT5 Antibody antibody |
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| STJ114819 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. |
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Anti-Anti-MARCH8 Antibody antibody |
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| STJ114828 | St John's Laboratory | 100 µl | EUR 277 |
Anti-Anti-DDB1 Antibody |
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| A00333 | BosterBio | 100uL | EUR 455 |
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Description: Rabbit Polyclonal DDB1 Antibody. Validated in IP and tested in Human, Mouse. |
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Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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| AEA465Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 5647.8 |
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Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids. |
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Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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| AEA465Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 552.76 |
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Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids. |
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Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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| AEA465Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 746.8 |
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Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids. |
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Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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| AEA465Hu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 3060.6 |
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Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids. |
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Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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| 4-AEA465Hu | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species. |
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ELISA kit for Human Anti-AsAb (Anti-Anti-Sperm Antibody Antibody) |
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| ELK8071 | ELK Biotech | 1 plate of 96 wells | EUR 432 |
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Description: A competitive Inhibition ELISA kit for detection of Anti-Anti-Sperm Antibody Antibody from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
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Anti-MeCP2 Antibody |
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| M00047-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal MeCP2 Antibody. Validated in IHC, WB and tested in Human, Monkey, Mouse, Rat. |
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Anti-GAPDH Antibody |
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| M00227-4 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal GAPDH Antibody. Validated in IF, IHC, WB and tested in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat. |
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Anti-Vimentin Antibody |
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| M00235-4 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Vimentin Antibody. Validated in IHC, WB and tested in Bovine, Equine, Human, Mouse, Pig, Rat. |
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Anti-Vimentin Antibody |
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| M00235-5 | BosterBio | 100ul | EUR 397 |
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Description: Chicken Polyclonal Vimentin Antibody. Validated in IHC, WB and tested in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat. |
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Anti-CNP Antibody |
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| M01017-2 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal CNP Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |
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Anti-CNP Antibody |
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| M01017-3 | BosterBio | 100ul | EUR 397 |
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Description: Chicken Polyclonal CNP Antibody. Validated in IHC, WB and tested in Equine, Pig. |
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Anti-GAP43 Antibody |
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| M01868-3 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal GAP43 Antibody. Validated in IF, IHC, WB and tested in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat. |
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Anti-GAP43 Antibody |
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| M01868-4 | BosterBio | 100ul | EUR 397 |
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Description: Chicken Polyclonal GAP43 Antibody. Validated in IF, IHC, WB and tested in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat. |
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Anti-GPSN2 Antibody |
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| F08289-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for GPSN2 Antibody (TECR) detection.tested for WB in Human, Mouse, Rat. |
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Anti-Calbindin Antibody |
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| M03047-2 | BosterBio | 100ul | EUR 397 |
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Description: Chicken Polyclonal Calbindin Antibody. Validated in IF, IHC, WB and tested in Bovine, Human, Mouse, Rat. |
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Anti-Fibrillarin Antibody |
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| M03178-4 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Fibrillarin Antibody. Validated in IP, IHC, WB and tested in Human, Mouse, Rat. |
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Anti-Fibrillarin Antibody |
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| M03178-5 | BosterBio | 100ul | EUR 397 |
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Description: Chicken Polyclonal Fibrillarin Antibody. Validated in IF, IHC, WB and tested in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat. |
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Anti-Parvalbumin Antibody |
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| M04041-2 | BosterBio | 100ul | EUR 397 |
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Description: Chicken Polyclonal Parvalbumin Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat. |
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Anti-Calretinin Antibody |
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| M04255-3 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Calretinin Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |
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Anti-Calretinin Antibody |
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| M04255-4 | BosterBio | 100ul | EUR 397 |
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Description: Chicken Polyclonal Calretinin Antibody. Validated in IF, IHC, WB and tested in Bovine, Equine, Human, Mouse, Pig, Rat. |
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Anti-Laminin Antibody |
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| M04965 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Laminin Antibody. Validated in IHC and tested in Human, Mouse, Rat. |
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Anti-Secretagogin Antibody |
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| M10629-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Secretagogin Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat. |
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Anti-Secretagogin Antibody |
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| M10629-2 | BosterBio | 100ul | EUR 397 |
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Description: Chicken Polyclonal Secretagogin Antibody. Validated in IF, IHC, WB and tested in Bovine, Human, Mouse, Rat. |
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Anti-ATF6 Antibody |
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| PA1011 | BosterBio | 100ug/vial | EUR 334 |
Anti-ATF6 Antibody |
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| PA1011-1 | BosterBio | 100ug/vial | EUR 294 |
Anti-ATP5J Antibody |
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| PA1012 | BosterBio | 100ug/vial | EUR 294 |
Anti-Bax Antibody |
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| PA1013 | BosterBio | 100ug/vial | EUR 294 |
Anti-Bax Antibody |
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| PA1013-1 | BosterBio | 100ug/vial | EUR 294 |
Anti-BDNF Antibody |
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| PA1014 | BosterBio | 100ug/vial | EUR 294 |
Anti-CCR5 Antibody |
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| PA1016 | BosterBio | 100ug/vial | EUR 294 |
Anti-CCR5 Antibody |
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| PA1016-1 | BosterBio | 100ug/vial | EUR 294 |
Anti-CCR5 Antibody |
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| PA1016-2 | BosterBio | 100ug/vial | EUR 294 |
Anti-CCR7 Antibody |
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| PA1017 | BosterBio | 100ug/vial | EUR 294 |
Anti-CD22 Antibody |
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| PA1018 | BosterBio | 100ug/vial | EUR 334 |
Anti-CD22 Antibody |
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| PA1018-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-CD40 Antibody |
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| PA1019 | BosterBio | 100ug/vial | EUR 294 |
Anti-CD40 Antibody |
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| PA1019-1 | BosterBio | 100ug/vial | EUR 294 |
Anti-CD44 Antibody |
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| PA1021 | BosterBio | 100ug/vial | EUR 334 |
Anti-CD44 Antibody |
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| PA1021-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-CD44 Antibody |
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| PA1021-2 | BosterBio | 100ug/vial | EUR 334 |
Anti-GJB2 Antibody |
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| PA1025 | BosterBio | 100ug/vial | EUR 294 |
Anti-CXCR2 Antibody |
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| PA1029 | BosterBio | 100ug/vial | EUR 294 |
Anti-dUTPase Antibody |
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| PA1030 | BosterBio | 100ug/vial | EUR 334 |
Anti-DUT Antibody |
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| PA1030-1 | BosterBio | 100ug/vial | EUR 334 |