Present Classification of the Bacillus pumilus Group Species, the Rubber-Pathogenic Micro organism Inflicting Trunk Bulges Illness in Malaysia as Assessed by MLSA and Multi rep-PCR Approaches
Bacillus pumilus is the causal agent of trunk bulges illness affecting rubber and rubberwood high quality and yield manufacturing. On this examine, B. pumilus and different carefully associated species had been included in B. pumilus group, as they shared over 99.5% similarity from 16S rRNA evaluation. Multilocus sequence evaluation (MLSA) of 5 housekeeping genes and repetitive elements-based polymerase chain response (rep-PCR) utilizing REP, ERIC, and BOX primers carried out to investigate the variety and systematic relationships of 20 isolates of B. pumilus group from 4 rubber tree plantations in Peninsular Malaysia (Serdang, Tanah Merah, Baling, and Rawang).
Multi rep-PCR outcomes revealed the genetic profiling among the many B. pumilus group isolates, whereas MLSA outcomes confirmed 98-100% similarity throughout the 20 isolates of B. pumilus group species. These 20 isolates, previously established as B. pumilus, had been discovered to not be grouped with B. pumilus. Nonetheless, being distributed inside distinctive teams of the B. pumilus group comprising of two clusters, A and B. Cluster A contained of 17 isolates near B. altitudinis, whereas Cluster B consisted of three isolates attributed to B. safensis. That is the primary MLSA and rep-PCR examine on B. pumilus group, which offers an in-depth understanding of the variety of those rubber-pathogenic isolates in Malaysia.

Anti-Relaxin 1 (1H6) |
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YF-MA15197 | Abfrontier | 100 ug | EUR 363 |
Description: Mouse monoclonal to Relaxin 1 |
Recombinant Human Relaxin-3 Protein |
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PROTQ8WXF3-1 | BosterBio | 25ug | EUR 317 |
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as “somatostatin-like” or “angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an “orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain. |
Anti-VEGF Receptor 3/FLT4 Antibody |
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A01276-3 | BosterBio | 100ug/vial | EUR 334 |
Anti-Relaxin 1/RLN1 Antibody |
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A08367-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-Relaxin Receptor 2 Antibody |
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A04848 | BosterBio | 100ul | EUR 397 |
Description: Rabbit Polyclonal Antibody for Relaxin Receptor 2 Antibody (RXFP2) detection.tested for WB in Human, Mouse, Rat. |
Anti-Relaxin Receptor 2 antibody |
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STJ95407 | St John's Laboratory | 200 µl | EUR 197 |
Description: Rabbit polyclonal to Relaxin Receptor 2. |
Anti-Relaxin Receptor 4 antibody |
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STJ95409 | St John's Laboratory | 200 µl | EUR 197 |
Description: Rabbit polyclonal to Relaxin Receptor 4. |
Anti-Relaxin 1 Antibody BIOTIN |
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STJ502817 | St John's Laboratory | 100 µg | EUR 586 |
Anti-Relaxin 1 Antibody FITC |
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STJ502818 | St John's Laboratory | 100 µg | EUR 586 |
Anti-14-3-3 alpha + beta Rabbit Monoclonal Antibody |
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M02431-3 | BosterBio | 100ug/vial | EUR 397 |
Description: Rabbit Monoclonal 14-3-3 alpha + beta Antibody. Validated in Flow Cytometry, IP, IF, IHC, ICC, WB and tested in Human, Mouse, Rat. |
Relaxin 3 Polyclonal Antibody |
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ES4671-50ul | ELK Biotech | 50ul | EUR 207 |
Description: A Rabbit Polyclonal antibody against Relaxin 3 from Human. This antibody is tested and validated for IHC, WB, ELISA |
Relaxin 3 Polyclonal Antibody |
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ES4671-100ul | ELK Biotech | 100ul | EUR 279 |
Description: A Rabbit Polyclonal antibody against Relaxin 3 from Human. This antibody is tested and validated for IHC, WB, ELISA |
Relaxin 3 (RLN3) Antibody |
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20-abx130221 | Abbexa |
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Relaxin 3 (RLN3) Antibody |
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20-abx121705 | Abbexa |
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Relaxin 3 Polyclonal Antibody |
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ABP53672-003ml | Abbkine | 0.03ml | EUR 158 |
Description: A polyclonal antibody for detection of Relaxin 3 from Human. This Relaxin 3 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin 3 |
Relaxin 3 Polyclonal Antibody |
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ABP53672-01ml | Abbkine | 0.1ml | EUR 289 |
Description: A polyclonal antibody for detection of Relaxin 3 from Human. This Relaxin 3 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin 3 |
Relaxin 3 Polyclonal Antibody |
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ABP53672-02ml | Abbkine | 0.2ml | EUR 414 |
Description: A polyclonal antibody for detection of Relaxin 3 from Human. This Relaxin 3 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin 3 |
Relaxin 3 (RLN3) Antibody |
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20-abx323035 | Abbexa |
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Relaxin 3 (RLN3) Antibody |
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20-abx218313 | Abbexa |
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Relaxin 3 Blocking Peptide |
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20-abx161415 | Abbexa |
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Relaxin 3 (RLN3) Antibody |
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20-abx178243 | Abbexa |
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Relaxin 3 (RLN3) Antibody |
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20-abx178244 | Abbexa |
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Relaxin 3 (RLN3) Antibody |
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20-abx174368 | Abbexa |
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Recombinant Relaxin 3 (RLN3) |
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4-RPD869Ra01 | Cloud-Clone |
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Description: Recombinant Rat Relaxin 3 expressed in: E.coli |
Relaxin-3 Recombinant Protein |
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40-230-0005mg | ProSci | 0.005 mg | EUR 259.25 |
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as "somatostatin-like” or "angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an "orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain. |
Relaxin-3 Recombinant Protein |
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40-230-0025mg | ProSci | 0.025 mg | EUR 364.25 |
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as "somatostatin-like” or "angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an "orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain. |
Relaxin H2 (human) |
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H-6784.0200 | Bachem | 0.2mg | EUR 618 |
Description: Sum Formula: C256H408N74O74S8; CAS# [99489-94-8] net |
Relaxin H2 (human) |
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H-6784.1000 | Bachem | 1.0mg | EUR 2378 |
Description: Sum Formula: C256H408N74O74S8; CAS# [99489-94-8] net |
Relaxin H3 (human) |
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H-7322.0200 | Bachem | 0.2mg | EUR 618 |
Description: Sum Formula: C237H374N70O69S6; CAS# [1158181-62-4] net |
Relaxin H3 (human) |
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H-7322.1000 | Bachem | 1.0mg | EUR 2378 |
Description: Sum Formula: C237H374N70O69S6; CAS# [1158181-62-4] net |
Anti-Human Ki67 Antibody |
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M00254-3 | BosterBio | 100ul | EUR 397 |
Description: Rabbit Polyclonal Human Ki67 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat. |
Anti-Human IBA1 Antibody |
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M01394-3 | BosterBio | 100ul | EUR 397 |
Description: Chicken Polyclonal Human IBA1 Antibody. Validated in IF, IHC, WB and tested in Human. |
Anti-active Caspase-3 Rabbit Monoclonal Antibody |
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M00334-3 | BosterBio | 100ug/vial | EUR 397 |
Description: Anti-active Caspase-3 Rabbit Monoclonal Antibody tested for IF, IHC, ICC, WB in Human |
Anti-Galectin 3/LGALS3 Antibody (monoclonal, 12B12) |
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M00621-3 | BosterBio | 100ug/vial | EUR 334 |
Polyclonal Goat anti-GST α-form |
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GST-ANTI-1 | Detroit R&D | 50 uL | EUR 280 |
Polyclonal Goat anti-GST μ-form |
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GST-ANTI-2 | Detroit R&D | 50 uL | EUR 280 |
Human Relaxin 3 (RLN3) ELISA Kit |
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abx512123-96tests | Abbexa | 96 tests | EUR 754 |
Human RLN3/ Relaxin-3 ELISA Kit |
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E2158Hu | Sunlong | 1 Kit | EUR 571 |
Human Relaxin 3 (RLN3) ELISA Kit |
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abx253713-96tests | Abbexa | 96 tests | EUR 754 |
RLN3 Relaxin-3 Human Recombinant Protein |
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PROTQ8WXF3 | BosterBio | Regular: 25ug | EUR 317 |
Description: Relaxin-3 Human Recombinant produced in E.Coli is a disulfide-linked heterodimeric, non-glycosylated, polypeptide chain containing 24 amino acids for A chain and 27 amino acids for B chain and having a molecular mass of 2.5kDa for A chain and 3kDa for B chain.;The Relaxin-3 is purified by proprietary chromatographic techniques. |
ELISA kit for Human Relaxin 3 |
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EK5653 | SAB | 96 tests | EUR 553 |
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Relaxin 3 in samples from serum, plasma, tissue homogenates and other biological fluids. |
Human Relaxin 3(RLN3)ELISA Kit |
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QY-E01376 | Qayee Biotechnology | 96T | EUR 361 |
Human RLN3(Relaxin-3) ELISA Kit |
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EH0755 | FN Test | 96T | EUR 567.6 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
Anti-Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Monoclonal Antibody |
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M00227-3 | BosterBio | 100ul | EUR 397 |
Description: Mouse Monoclonal Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Antibody. Validated in IHC, WB and tested in Equine. |
Relaxin Receptor 3 Polyclonal Antibody |
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ES6513-100ul | ELK Biotech | 100ul | EUR 279 |
Description: A Rabbit Polyclonal antibody against Relaxin Receptor 3 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA |
Relaxin Receptor 3 Polyclonal Antibody |
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ES6513-50ul | ELK Biotech | 50ul | EUR 207 |
Description: A Rabbit Polyclonal antibody against Relaxin Receptor 3 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA |
Relaxin Receptor 3 Polyclonal Antibody |
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ABP55514-003ml | Abbkine | 0.03ml | EUR 158 |
Description: A polyclonal antibody for detection of Relaxin Receptor 3 from Human, Mouse, Rat. This Relaxin Receptor 3 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin Receptor 3 at AA range: 130-210 |
Relaxin Receptor 3 Polyclonal Antibody |
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ABP55514-01ml | Abbkine | 0.1ml | EUR 289 |
Description: A polyclonal antibody for detection of Relaxin Receptor 3 from Human, Mouse, Rat. This Relaxin Receptor 3 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin Receptor 3 at AA range: 130-210 |
Relaxin Receptor 3 Polyclonal Antibody |
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ABP55514-02ml | Abbkine | 0.2ml | EUR 414 |
Description: A polyclonal antibody for detection of Relaxin Receptor 3 from Human, Mouse, Rat. This Relaxin Receptor 3 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin Receptor 3 at AA range: 130-210 |
Relaxin 3 receptor 1 Antibody |
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abx019161-100ug | Abbexa | 100 ug | EUR 342 |
Rat Relaxin 3 (RLN3) Protein |
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20-abx168490 | Abbexa |
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Mouse Relaxin 3 (RLN3) Protein |
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20-abx654935 | Abbexa |
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Mouse Relaxin 3 (RLN3) Protein |
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20-abx654936 | Abbexa |
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Relaxin 3 receptor 1 Antibody |
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48026-100ul | SAB | 100ul | EUR 333 |
Relaxin 3 receptor 1 Antibody |
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48026-50ul | SAB | 50ul | EUR 239 |
ELISA kit for Human RLN3 (Relaxin-3) |
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E-EL-H1652 | Elabscience Biotech | 1 plate of 96 wells | EUR 534 |
Description: A sandwich ELISA kit for quantitative measurement of Human RLN3 (Relaxin-3) in samples from Serum, Plasma, Cell supernatant |
ELISA kit for Human Relaxin-3 (RLN3) |
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KTE60790-48T | Abbkine | 48T | EUR 332 |
Description: Quantitative sandwich ELISA for measuring Human Relaxin-3 (RLN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Relaxin-3 (RLN3) |
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KTE60790-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2115 |
Description: Quantitative sandwich ELISA for measuring Human Relaxin-3 (RLN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Relaxin-3 (RLN3) |
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KTE60790-96T | Abbkine | 96T | EUR 539 |
Description: Quantitative sandwich ELISA for measuring Human Relaxin-3 (RLN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
Human Relaxin (RLN) Protein |
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20-abx654931 | Abbexa |
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Human Relaxin ELISA Kit |
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ELA-E1216h | Lifescience Market | 96 Tests | EUR 824 |
Relaxin-2, human recombinant |
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7194-10 | Biovision | EUR 207 |
Relaxin-2, human recombinant |
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7194-50 | Biovision | EUR 675 |
Human Relaxin ELISA kit |
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E01R0008-192T | BlueGene | 192 tests | EUR 1270 |
Description: A competitive ELISA for quantitative measurement of Human Relaxin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Relaxin ELISA kit |
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E01R0008-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
Description: A competitive ELISA for quantitative measurement of Human Relaxin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Relaxin ELISA kit |
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E01R0008-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
Description: A competitive ELISA for quantitative measurement of Human Relaxin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Anti-MRP1 (human) Monoclonal Antibody (QCRL-1) |
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M00872-3 | BosterBio | 1ml | EUR 675 |
Description: Mouse Monoclonal MRP1 (human) Antibody (QCRL-1). Validated in IP, IF and tested in Human. |
Porcine relaxin peptides / relaxin (RLN) ELISA Kit |
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QY-E40036 | Qayee Biotechnology | 96T | EUR 400 |
Anti-Human IgM Rabbit Monoclonal Antibody, Clone#RM121 |
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M07469-3 | BosterBio | 100ug | EUR 375 |
Description: Anti-Human IgM Rabbit Monoclonal Antibody, Clone#RM121 tested in ICC, IHC, FC, ELISA, reactive to Human |
Anti-CNP Antibody |
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M01017-3 | BosterBio | 100ul | EUR 397 |
Description: Chicken Polyclonal CNP Antibody. Validated in IHC, WB and tested in Equine, Pig. |
Anti-GAP43 Antibody |
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M01868-3 | BosterBio | 100ul | EUR 397 |
Description: Rabbit Polyclonal GAP43 Antibody. Validated in IF, IHC, WB and tested in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat. |
Anti-Calretinin Antibody |
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M04255-3 | BosterBio | 100ul | EUR 397 |
Description: Rabbit Polyclonal Calretinin Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |
Anti-Nanog Antibody |
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A00153-3 | BosterBio | 100ug/vial | EUR 294 |
Anti-IL17C Antibody |
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A00164-3 | BosterBio | 100ug/vial | EUR 294 |
Anti-AICDA Antibody |
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A00267-3 | BosterBio | 100ug/vial | EUR 294 |
Anti-ICOS Antibody |
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A00291-3 | BosterBio | 100ug/vial | EUR 334 |
Anti-TLR1 Antibody |
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A00429-3 | BosterBio | 100ug/vial | EUR 334 |
Anti-IGFBP3 Antibody |
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A00435-3 | BosterBio | 100ug/vial | EUR 294 |
Anti-IL17B Antibody |
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A10846-3 | BosterBio | 100ug/vial | EUR 294 |
Anti-RAB3GAP2 Antibody |
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A07244-3 | BosterBio | 100ug/vial | EUR 334 |
Anti-FOXI1 Antibody |
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A07512-3 | BosterBio | 100ug/vial | EUR 334 |
Anti-TANK Antibody |
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A00445-3 | BosterBio | 100ug/vial | EUR 334 |
Anti-FGF23 Antibody |
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A00478-3 | BosterBio | 100ug/vial | EUR 294 |
Anti-Leptin Antibody |
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A00479-3 | BosterBio | 100ug/vial | EUR 294 |
Anti-CD5 Antibody |
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A00480-3 | BosterBio | 100ug/vial | EUR 334 |
Anti-SYK Antibody |
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A00490-3 | BosterBio | 100ug/vial | EUR 294 |
Anti-PON1 Antibody |
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A00516-3 | BosterBio | 100ug/vial | EUR 334 |
Anti-CSF1 Antibody |
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A00620-3 | BosterBio | 100ug/vial | EUR 294 |
Anti-LBP Antibody |
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A00809-3 | BosterBio | 100ug/vial | EUR 294 |
Anti-IL22 Antibody |
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A00963-3 | BosterBio | 100ug/vial | EUR 294 |
Design of a digital-PCR assay to quantify fragmented human mitochondrial DNA
Digital PCR (dPCR) has been tailored to quantify the proportion of mitochondrial DNA (mtDNA) molecules with out and with double-strand DNA breaks (DSBs). That is based mostly on a break-apart strategy of two differentially labeled goal sequences distantly situated within the round DNA molecule. When the 2 targets amplify in separated response partitions, the unique mtDNA molecule ought to be fragmented by two DSBs not less than, every in a unique phase between targets.
When each targets amplify in the identical partition, it should correspond to a round or linear mtDNA molecule. These two prospects could also be distinguished via a restriction endonuclease (RE) induced distinctive DSB inside a DNA phase between the targets. After RE-digestion, separation of each goal alerts in several partitions should point out the presence of a earlier linear mtDNA molecule. In any other case, joint amplification in the identical partition would correspond to an preliminary round mtDNA, that has been linearized by the endonuclease.
The process was validated by assaying totally different proportions of mtDNA fragmented by in vitro digestion with REs, evidencing an ideal accordance beween the anticipated theoretical values and dPCR quantification. Samples from peripheral blood cells, mobile and extracellular DNA from the U2OS cell line, in addition to cells incubated with ethidium bromide to induce mtDNA depletion, had been evaluated. The method could also be of curiosity to enhance the research of mtDNA in relation to growing older and human illness, in addition to to evaluate potential antagonistic results of sure medication that may very well be associated to affectation of mtDNA. This text is protected
Comparability of mitochondrial genome and improvement of particular PCR primers for figuring out two scuticociliates, Pseudocohnilembus persalinus and Uronema marinum
Background: Pseudocohnilembus persalinus and Uronema marinum (Ciliophora, Scuticociliatia), as parasitic scuticociliatid ciliates, had been remoted from Scophthalmus maximus and Takifugu rubripes, respectively, in our earlier research. These ciliates are morphologically very comparable; therefore, it’s troublesome to determine particular scuticociliate species utilizing conventional classification strategies for performing taxonomic analysis and illness management research.
Strategies: We annotated the mitochondrial genomes of those two scuticociliates on the premise of earlier sequencing, together with analyses of nucleotide composition, codon utilization, Ka/Ks, and p-distance. We additionally in contrast the nucleotide and amino acid similarity of the mitochondrial genomes of P. persalinus, U. marinum, and different 12 associated ciliates, and a phylogenetic tree was constructed utilizing 16 widespread genes. We selected the nad4 and nad7 genes to design particular PCR primers for identification.
Outcomes: P. persalinus and U. marinum had been discovered to have a detailed evolutionary relationship. Though codon preferences had been comparable, variations had been noticed within the utilization of codons reminiscent of CGA, CGC, and GTC. Each Ka/Ks and p-distance had been lower than 1. Apart from yejR, ymf57, ymf67, and ymf75, the amino acid sequence similarity between P. persalinus and U. marinum was higher than 50%.
Conclusions: The mitochondrial genomes of P. persalinus and U. marinum had been completely in contrast to offer a reference for illness prevention and management. The precise PCR primers enabled us to determine P. persalinus and U. marinum quickly and precisely on the molecular degree, thus offering a foundation for classification and identification.
Estimating Clinically Related Minimize-Off Values for a Excessive-Throughput Quantitative Actual-Time PCR Detecting Bacterial Respiratory Pathogens in Cattle
Bovine respiratory illness (BRD) outcomes from interactions between pathogens, environmental stressors, and host elements. Acquiring a analysis of the causal pathogens is difficult however using high-throughput real-time PCR (rtPCR) might assist goal preventive and therapeutic interventions.
The intention of this examine was to enhance the interpretation of rtPCR outcomes by analysing their associations with medical observations. The target was to develop and illustrate a field-data pushed statistical technique to information the collection of related quantification cycle cut-off values for pathogens related to BRD for the high-throughput rtPCR system “Fluidigm BioMark HD” based mostly on nasal swabs from calves. We used information from 36 herds enrolled in a Danish subject examine the place 340 calves inside pre-determined age-groups had been topic to medical examination and nasal swabs as much as 4 occasions.
The samples had been analysed with the rtPCR system. Every of the 1,025 statement models had been categorised as sick with BRD or wholesome, based mostly on medical scores. The optimum rtPCR outcomes to foretell BRD had been investigated for Pasteurella multocida, Mycoplasma bovis, Histophilus somni, Mannheimia haemolytica, and Trueperella pyogenes by decoding scatterplots and outcomes of blended results logistic regression fashions.
The clinically related rtPCR cut-off prompt for P. multocida and M. bovis was ≤ 21.3. For H. somni it was ≤ 17.4, whereas no cut-off may very well be decided for M. haemolytica and T. pyogenes. The demonstrated strategy can present goal help within the alternative of clinically related cut-offs. Nonetheless, for strong efficiency of the regression mannequin enough quantities of appropriate information are required.
dsGreen for Real-Time PCR, 100×, 100 uL |
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11010 | Lumiprobe | 100 uL | EUR 59 |
dsGreen for Real-Time PCR, 100×, 1 mL |
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41010 | Lumiprobe | 1 mL | EUR 249 |
dsGreen for Real-Time PCR, 100×, 1.5 mL |
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51010 | Lumiprobe | 1.5 mL | EUR 331 |
dsGreen for Real-Time PCR, 100×, 2 mL |
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61010 | Lumiprobe | 2 mL | EUR 363 |
dsGreen for Real-Time PCR, 100×, 10 mL |
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81010 | Lumiprobe | 10 mL | EUR 869 |
dsGreen for Real-Time PCR, 100×, 50 mL |
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91010 | Lumiprobe | 50 mL | EUR 3169 |
dsGreen for Real-Time PCR, 100×, 5 mL |
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71010 | Lumiprobe | 5 mL | EUR 625 |
hsa-let-7a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097603 | Abbexa |
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hsa-let-7b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097604 | Abbexa |
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hsa-let-7c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097605 | Abbexa |
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hsa-let-7d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097606 | Abbexa |
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hsa-let-7e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097607 | Abbexa |
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hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097608 | Abbexa |
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hsa-let-7g Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097609 | Abbexa |
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hsa-let-7i Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097610 | Abbexa |
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hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097611 | Abbexa |
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hsa-mir-1 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097612 | Abbexa |
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hsa-mir-7 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097613 | Abbexa |
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hsa-mir-9 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097614 | Abbexa |
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hsa-mir-9* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097615 | Abbexa |
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hsa-mir-10a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097616 | Abbexa |
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hsa-mir-10b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097617 | Abbexa |
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hsa-mir-10a* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097618 | Abbexa |
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hsa-mir-15a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097619 | Abbexa |
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hsa-mir-15b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097620 | Abbexa |
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hsa-mir-16 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097622 | Abbexa |
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hsa-mir-18a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097625 | Abbexa |
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hsa-mir-18b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097626 | Abbexa |
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hsa-mir-19a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097627 | Abbexa |
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hsa-mir-19b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097628 | Abbexa |
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hsa-mir-20a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097629 | Abbexa |
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hsa-mir-20b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097630 | Abbexa |
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hsa-mir-21 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097631 | Abbexa |
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hsa-mir-22 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097632 | Abbexa |
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hsa-mir-23a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097633 | Abbexa |
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hsa-mir-23b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097634 | Abbexa |
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hsa-mir-25 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097636 | Abbexa |
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hsa-mir-26a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097637 | Abbexa |
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hsa-mir-26b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097638 | Abbexa |
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hsa-mir-27a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097639 | Abbexa |
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hsa-mir-27b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097640 | Abbexa |
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hsa-mir-29a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097642 | Abbexa |
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hsa-mir-29b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097643 | Abbexa |
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hsa-mir-29c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097644 | Abbexa |
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hsa-mir-30a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097645 | Abbexa |
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hsa-mir-30b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097647 | Abbexa |
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hsa-mir-30c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097648 | Abbexa |
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hsa-mir-30d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097650 | Abbexa |
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hsa-mir-30e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097651 | Abbexa |
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hsa-mir-31 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097652 | Abbexa |
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hsa-mir-32 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097653 | Abbexa |
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hsa-mir-33a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097654 | Abbexa |
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hsa-mir-34a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097657 | Abbexa |
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hsa-mir-34b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097659 | Abbexa |
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hsa-mir-92a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097661 | Abbexa |
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hsa-mir-92b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097662 | Abbexa |
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hsa-mir-93 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097663 | Abbexa |
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hsa-mir-95 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097664 | Abbexa |
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hsa-mir-96 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097665 | Abbexa |
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hsa-mir-98 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097666 | Abbexa |
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hsa-mir-99a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097667 | Abbexa |
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hsa-mir-99b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097669 | Abbexa |
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hsa-mir-100 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097671 | Abbexa |
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hsa-mir-101 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097673 | Abbexa |
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hsa-mir-103 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097675 | Abbexa |
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hsa-mir-105 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097676 | Abbexa |
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hsa-mir-106a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097677 | Abbexa |
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hsa-mir-106b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097678 | Abbexa |
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hsa-mir-107 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097679 | Abbexa |
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hsa-mir-124 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097680 | Abbexa |
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hsa-mir-125a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097681 | Abbexa |
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hsa-mir-127 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097686 | Abbexa |
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hsa-mir-129 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097689 | Abbexa |
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hsa-mir-130a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097690 | Abbexa |
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hsa-mir-130b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097691 | Abbexa |
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hsa-mir-132 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097692 | Abbexa |
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hsa-mir-133a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097693 | Abbexa |
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hsa-mir-133b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097694 | Abbexa |
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hsa-mir-134 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097695 | Abbexa |
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hsa-mir-135b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097696 | Abbexa |
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hsa-mir-137 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097699 | Abbexa |
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hsa-mir-138 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097700 | Abbexa |
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hsa-mir-139 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097701 | Abbexa |
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hsa-mir-140 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097702 | Abbexa |
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hsa-mir-141 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097703 | Abbexa |
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hsa-mir-143 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097706 | Abbexa |
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hsa-mir-145 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097708 | Abbexa |
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hsa-mir-146a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097709 | Abbexa |
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hsa-mir-149 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097711 | Abbexa |
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hsa-mir-150 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097712 | Abbexa |
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hsa-mir-151 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097714 | Abbexa |
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hsa-mir-152 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097715 | Abbexa |
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