Present Classification of the Bacillus pumilus Group Species, the Rubber-Pathogenic Micro organism Inflicting Trunk Bulges Illness in Malaysia as Assessed by MLSA and Multi rep-PCR Approaches
Bacillus pumilus is the causal agent of trunk bulges illness affecting rubber and rubberwood high quality and yield manufacturing. On this examine, B. pumilus and different carefully associated species had been included in B. pumilus group, as they shared over 99.5% similarity from 16S rRNA evaluation. Multilocus sequence evaluation (MLSA) of 5 housekeeping genes and repetitive elements-based polymerase chain response (rep-PCR) utilizing REP, ERIC, and BOX primers carried out to investigate the variety and systematic relationships of 20 isolates of B. pumilus group from 4 rubber tree plantations in Peninsular Malaysia (Serdang, Tanah Merah, Baling, and Rawang).
Multi rep-PCR outcomes revealed the genetic profiling among the many B. pumilus group isolates, whereas MLSA outcomes confirmed 98-100% similarity throughout the 20 isolates of B. pumilus group species. These 20 isolates, previously established as B. pumilus, had been discovered to not be grouped with B. pumilus. Nonetheless, being distributed inside distinctive teams of the B. pumilus group comprising of two clusters, A and B. Cluster A contained of 17 isolates near B. altitudinis, whereas Cluster B consisted of three isolates attributed to B. safensis. That is the primary MLSA and rep-PCR examine on B. pumilus group, which offers an in-depth understanding of the variety of those rubber-pathogenic isolates in Malaysia.
Relaxin-3, human recombinant |
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| 7195-10 | Biovision | each | EUR 248.4 |
Relaxin-3, human recombinant |
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| 7195-50 | Biovision | each | EUR 810 |
Relaxin-3 Human Recombinant |
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| rAP-4650 | Angio Proteomie | Inquiry | Ask for price |
INSL7 / Relaxin 3 (Human) |
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| 035-36 | PHOENIX PEPTIDE | 20 μg | EUR 160.92 |
INSL7 / Relaxin 3 (Human) |
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| 035-36A | PHOENIX PEPTIDE | 100 μg | EUR 604.8 |
Human Relaxin-3,RLN-3 ELISA |
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| YLA4309HU-48T | Shanghai YL Biotech | 48T | Ask for price |
Human Relaxin-3,RLN-3 ELISA |
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| YLA4309HU-96T | Shanghai YL Biotech | 96T | Ask for price |
Human Relaxin-3 ELISA Kit |
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| MBS765502-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3900 |
Human Relaxin-3 ELISA Kit |
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| MBS765502-48StripWells | MyBiosource | 48-Strip-Wells | EUR 340 |
Human Relaxin-3 ELISA Kit |
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| MBS765502-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 2045 |
Human Relaxin-3 ELISA Kit |
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| MBS765502-96StripWells | MyBiosource | 96-Strip-Wells | EUR 455 |
Human Relaxin-3 ELISA Kit |
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| MBS2887639-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 4080 |
Human Relaxin-3 ELISA Kit |
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| MBS2887639-48StripWells | MyBiosource | 48-Strip-Wells | EUR 390 |
Human Relaxin-3 ELISA Kit |
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| MBS2887639-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 2220 |
Human Relaxin-3 ELISA Kit |
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| MBS2887639-96StripWells | MyBiosource | 96-Strip-Wells | EUR 520 |
INSL7 / Relaxin 3 (Human) - Antibody |
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| H-035-36 | PHOENIX PEPTIDE | 50 μl | EUR 604.8 |
Mouse Monoclonal anti-human Relaxin-3 |
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| hAP-0526 | Angio Proteomie | 100ug | EUR 250 |
Human Relaxin 3 ELISA Kit |
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| EK5653 | SAB | 96 tests | EUR 599 |
Human Relaxin 3 ELISA Kit |
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| MBS054925-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Human Relaxin 3 ELISA Kit |
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| MBS054925-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Human Relaxin 3 ELISA Kit |
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| MBS054925-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Human Relaxin 3 ELISA Kit |
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| MBS054925-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Human relaxin 3 ELISA Kit |
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| SL2396Hu | Sunlong | 96 Tests | EUR 468 |
Human Relaxin 3 ELISA Kit |
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| MBS8248392-5x96Tests | MyBiosource | 5x96Tests | EUR 2880 |
Human Relaxin 3 ELISA Kit |
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| MBS8248392-96Tests | MyBiosource | 96Tests | EUR 645 |
Human Relaxin-3 Recombinant Protein |
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| 100-356 | ReliaTech | 25 µg | EUR 196.35 |
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Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as “somatostatin-like” or “angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an “orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain. |
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Human Relaxin-3 Recombinant Protein |
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| 100-356S | ReliaTech | 5 µg | EUR 92.4 |
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Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as “somatostatin-like” or “angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an “orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain. |
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Recombinant Human Relaxin-3 Protein |
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| PROTQ8WXF3-1 | BosterBio | 25ug | EUR 380.4 |
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Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as “somatostatin-like” or “angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an “orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain. |
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Human Relaxin 3 (RLN3) Protein |
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| abx680520-100g | Abbexa | 100 µg | EUR 225 |
Human Relaxin-3 (RLN3) ELISA Kit |
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| EK7514 | SAB | 96Т | EUR 799 |
Human Relaxin-3(RLN3) Elisa Kit |
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| EK713553 | AFG Bioscience LLC | 96 Wells | EUR 0.37 |
Human Relaxin-3 (RLN3) ELISA Kit |
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| AE22052HU-48Tests | Abebio | 48 Tests | EUR 325 |
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Description: Human (Homo sapiens) |
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Human Relaxin-3 (RLN3) ELISA Kit |
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| AE22052HU-96Tests | Abebio | 96 Tests | EUR 610 |
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Description: Human (Homo sapiens) |
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Human Relaxin-3 (RLN3) ELISA Kit |
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| AE22052HU-48T | Abebio | 48T | EUR 325 |
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Description: Human (Homo sapiens) |
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Human Relaxin-3 (RLN3) ELISA Kit |
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| AE22052HU-96T | Abebio | 96T | EUR 610 |
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Description: Human (Homo sapiens) |
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Human Relaxin-3 (RLN3) ELISA Kit |
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| KTE60790-48T | Abbkine | 48T | EUR 339 |
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Description: This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3. |
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Human Relaxin-3 (RLN3) ELISA Kit |
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| KTE60790-96T | Abbkine | 96T | EUR 589 |
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Description: This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3. |
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Human Relaxin-3 (RLN3) ELISA Kit |
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| KTE60790-96T5 | Abbkine | 96 T×5 | EUR 2629 |
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Description: This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3. |
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Human Relaxin-3 (RLN3) ELISA Kit |
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| KTE60790-96T50 | Abbkine | 96 T×50 | EUR 24569 |
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Description: This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3. |
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Human Relaxin-3 (RLN3) ELISA Kit |
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| RK06347 | Abclonal | 96T | EUR 280 |
Human Relaxin-3 (RLN3) ELISA Kit |
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| MBS9714455-48Tests | MyBiosource | 48Tests | EUR 340 |
Human Relaxin-3 (RLN3) ELISA Kit |
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| MBS9714455-5x96Tests | MyBiosource | 5x96Tests | EUR 1820 |
Human Relaxin-3 (RLN3) ELISA Kit |
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| MBS9714455-96Tests | MyBiosource | 96Tests | EUR 500 |
Human Relaxin-1 |
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| hAP-0525 | Angio Proteomie | 50ug | EUR 400 |
Human Relaxin-2 |
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| MBS691734-0005mg | MyBiosource | 0.005mg | EUR 300 |
Human Relaxin-2 |
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| MBS691734-0025mg | MyBiosource | 0.025mg | EUR 450 |
Human Relaxin-2 |
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| MBS691734-5x0025mg | MyBiosource | 5x0.025mg | EUR 1725 |
Relaxin 3, Recombinant, Human (H3 Relaxin, Insulin-Like Peptide-7, INSL7) |
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| MBS650130-0005mg | MyBiosource | 0.005mg | EUR 340 |
Relaxin 3, Recombinant, Human (H3 Relaxin, Insulin-Like Peptide-7, INSL7) |
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| MBS650130-0025mg | MyBiosource | 0.025mg | EUR 490 |
Relaxin 3, Recombinant, Human (H3 Relaxin, Insulin-Like Peptide-7, INSL7) |
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| MBS650130-5x0025mg | MyBiosource | 5x0.025mg | EUR 2055 |
Relaxin-2 (Human) |
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| MBS408061-005mg | MyBiosource | 0.05mg | EUR 345 |
Relaxin-2 (Human) |
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| MBS408061-5x005mg | MyBiosource | 5x0.05mg | EUR 1500 |
Human RLN3(Relaxin-3) ELISA Kit |
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| EH0755 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human RLN3/ Relaxin-3 ELISA Kit |
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| E2158Hu | Sunlong | 1 Kit | EUR 515 |
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Description: UNQ6188/PRO20213,INSL7,RXN3,ZINS4,Insulin-like peptide INSL7,Prorelaxin H3,Insulin-like peptide 7 |
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Human RLN3 / Relaxin-3 ELISA Kit |
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| E0189h | EIAab | 96T | EUR 736 |
Human RLN3(Relaxin-3) ELISA Kit |
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| RE1961H-48wells | Reed Biotech | 48 wells | EUR 125.55 |
Human RLN3(Relaxin-3) ELISA Kit |
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| RE1961H-96wells | Reed Biotech | 96 wells | EUR 179.55 |
Human RLN3 (Relaxin-3) ELISA Kit |
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| MBS2516020-10x96Tests | MyBiosource | 10x96Tests | EUR 4060 |
Human RLN3 (Relaxin-3) ELISA Kit |
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| MBS2516020-24Tests | MyBiosource | 24Tests | EUR 265 |
Human RLN3 (Relaxin-3) ELISA Kit |
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| MBS2516020-48Test | MyBiosource | 48Test | EUR 450 |
Human RLN3 (Relaxin-3) ELISA Kit |
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| MBS2516020-5x96Test | MyBiosource | 5x96Test | EUR 2265 |
Human RLN3 (Relaxin-3) ELISA Kit |
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| MBS2516020-96Tests | MyBiosource | 96Tests | EUR 535 |
Human RLN3(Relaxin-3) ELISA Kit |
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| RE1961H-48wellsplate | Reed Biotech | 48 wells plate | EUR 126 |
Human RLN3(Relaxin-3) ELISA Kit |
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| RE1961H-96wellsplate | Reed Biotech | 96 wells plate | EUR 180 |
Human Relaxin 3 (RLN3) ELISA Kit |
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| abx253713-96tests | Abbexa | 96 tests | EUR 904.8 |
Human Relaxin 3 (RLN3) ELISA Kit |
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| abx512123-96tests | Abbexa | 96 tests | EUR 904.8 |
Human Relaxin 3,RLN3 ELISA KIT |
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| E7543Hu-1096T | Jiaxing Korain Biotech Ltd (BT Labs) | 10*96T | EUR 4122 |
Human Relaxin 3,RLN3 ELISA KIT |
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| E7543Hu-48wells | Jiaxing Korain Biotech Ltd (BT Labs) | 48 wells | EUR 300 |
Human Relaxin 3,RLN3 ELISA KIT |
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| E7543Hu-596T | Jiaxing Korain Biotech Ltd (BT Labs) | 5*96T | EUR 2061 |
Human Relaxin 3,RLN3 ELISA KIT |
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| E7543Hu-96wells | Jiaxing Korain Biotech Ltd (BT Labs) | 96 wells | EUR 458 |
Human Relaxin 3(RLN3)ELISA Kit |
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| QY-E01376 | Qayee Biotechnology | 96T | EUR 433.2 |
Human Relaxin 3, RLN3 ELISA Kit |
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| MBS1609027-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3460 |
Human Relaxin 3, RLN3 ELISA Kit |
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| MBS1609027-48StripWells | MyBiosource | 48-Strip-Wells | EUR 285 |
Human Relaxin 3, RLN3 ELISA Kit |
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| MBS1609027-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1750 |
Human Relaxin 3, RLN3 ELISA Kit |
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| MBS1609027-96StripWells | MyBiosource | 96-Strip-Wells | EUR 425 |
Nori® Human Relaxin-3 ELISA Kit |
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| GR111288 | Genorise Scientific | 96-well | EUR 461 |
Recombinant Human Relaxin-3 (RLN3), partial |
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| MBS7094488-005mgBaculovirus | MyBiosource | 0.05mg(Baculovirus) | EUR 885 |
Recombinant Human Relaxin-3 (RLN3), partial |
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| MBS7094488-005mgEColi | MyBiosource | 0.05mg(E-Coli) | EUR 510 |
Recombinant Human Relaxin-3 (RLN3), partial |
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| MBS7094488-02mgEColi | MyBiosource | 0.2mg(E-Coli) | EUR 665 |
Recombinant Human Relaxin-3 (RLN3), partial |
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| MBS7094488-02mgYeast | MyBiosource | 0.2mg(Yeast) | EUR 945 |
Recombinant Human Relaxin-3 (RLN3), partial |
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| MBS7094488-05mgEColi | MyBiosource | 0.5mg(E-Coli) | EUR 710 |
ELISA kit for Human Relaxin-3 (RLN3) |
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| KTE60790-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2538 |
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Description: Quantitative sandwich ELISA for measuring Human Relaxin-3 (RLN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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INSL7 / Relaxin 3 (Human) - RIA Kit |
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| RK-035-36 | PHOENIX PEPTIDE | 125 tubes | EUR 1061.64 |
Relaxin-3 |
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| AP87248 | SAB | 1mg | EUR 2640 |
Relaxin-3 |
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| AP87370 | SAB | 1mg | EUR 2640 |
Relaxin-3 |
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| AP87734 | SAB | 1mg | EUR 2640 |
Relaxin-3 |
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| AP88315 | SAB | 1mg | EUR 2640 |
Relaxin H3 (human) |
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| 4039569.02 | Bachem | 0.2 mg | EUR 455.07 |
Relaxin H3 (human) |
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| 4039569.1 | Bachem | 1 mg | EUR 1818.92 |
Relaxin H2 (human) |
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| 4039910.02 | Bachem | 0.2 mg | EUR 455.07 |
Relaxin H2 (human) |
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| 4039910.1 | Bachem | 1 mg | EUR 1818.92 |
Relaxin H2 (human) |
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| H-6784.0200 | Bachem | 0.2mg | EUR 741.6 |
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Description: Sum Formula: C256H408N74O74S8; CAS# [99489-94-8] net |
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Relaxin H2 (human) |
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| H-6784.1000 | Bachem | 1.0mg | EUR 2853.6 |
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Description: Sum Formula: C256H408N74O74S8; CAS# [99489-94-8] net |
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Relaxin H3 (human) |
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| H-7322.0200 | Bachem | 0.2mg | EUR 741.6 |
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Description: Sum Formula: C237H374N70O69S6; CAS# [1158181-62-4] net |
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Relaxin H3 (human) |
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| H-7322.1000 | Bachem | 1.0mg | EUR 2853.6 |
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Description: Sum Formula: C237H374N70O69S6; CAS# [1158181-62-4] net |
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Relaxin 2 (Human) |
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| 035-62 | PHOENIX PEPTIDE | 20 μg | EUR 261.36 |
Relaxin 2 (Human) |
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| 035-62A | PHOENIX PEPTIDE | 100 μg | EUR 469.8 |
Human RLN3(Relaxin 3) ELISA Kit |
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| ELK9324-48T | ELK Biotech | 48T | Ask for price |
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Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RLN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RLN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RLN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RLN3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Human RLN3(Relaxin 3) ELISA Kit |
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| ELK9324-96T | ELK Biotech | 96T | Ask for price |
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Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RLN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RLN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RLN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RLN3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Human RLN3 (Relaxin 3) ELISA Kit |
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| MBS8819812-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3130 |
Human RLN3 (Relaxin 3) ELISA Kit |
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| MBS8819812-48StripWells | MyBiosource | 48-Strip-Wells | EUR 350 |
Human RLN3 (Relaxin 3) ELISA Kit |
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| MBS8819812-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1710 |
Human RLN3 (Relaxin 3) ELISA Kit |
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| MBS8819812-96StripWells | MyBiosource | 96-Strip-Wells | EUR 445 |
Anti- Relaxin-3 receptor 2 Antibody |
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| GWB-214500 | GenWay Biotech | 0.05 ml | Ask for price |
Anti- Relaxin-3 receptor 2 Antibody |
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| GWB-EDDDF9 | GenWay Biotech | 0.05 ml | Ask for price |
Relaxin 2 (Human) - Antibody |
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| H-035-62 | PHOENIX PEPTIDE | 100 μl | EUR 604.8 |
Human Relaxin 3 PicoKine ELISA Kit |
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| EK1407 | BosterBio | 96 wells | EUR 510 |
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Description: For quantitative detection of human Relaxin 3 in cell culture supernates, serum and plasma( heparin). |
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RLN3 (untagged)-Human relaxin 3 (RLN3) |
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| SC305868 | Origene Technologies GmbH | 10 µg | Ask for price |
Human Relaxin 3 PicoKine ELISA Kit |
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| MBS177627-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3935 |
Human Relaxin 3 PicoKine ELISA Kit |
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| MBS177627-96StripWells | MyBiosource | 96-Strip-Wells | EUR 855 |
ELISA kit for Human RLN3 (Relaxin-3) |
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| E-EL-H1652 | Elabscience Biotech | 1 plate of 96 wells | EUR 640.8 |
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Description: A sandwich ELISA kit for quantitative measurement of Human RLN3 (Relaxin-3) in samples from Serum, Plasma, Cell supernatant |
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Relaxin 3 (BDelta23-27) R (Human) |
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| 036-20 | PHOENIX PEPTIDE | 100 μg | EUR 461.16 |
RLN3 Relaxin-3 Human Recombinant Protein |
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| PROTQ8WXF3 | BosterBio | Regular: 25ug | EUR 380.4 |
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Description: Relaxin-3 Human Recombinant produced in E.Coli is a disulfide-linked heterodimeric, non-glycosylated, polypeptide chain containing 24 amino acids for A chain and 27 amino acids for B chain and having a molecular mass of 2.5kDa for A chain and 3kDa for B chain.;The Relaxin-3 is purified by proprietary chromatographic techniques. |
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INSL7 / Relaxin 3 (Human) - Cy5 Labeled |
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| FC5-035-36 | PHOENIX PEPTIDE | 1 nmol | EUR 756 |
INSL7 / Relaxin 3 (Human) - Purified IgG Antibody |
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| G-035-36 | PHOENIX PEPTIDE | 200 μg | EUR 604.8 |
Human Relaxin-3 Recombinant Protein Lyophilized |
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| IHURLXN3RLY5UG | Innovative research | each | EUR 341 |
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Description: Human Relaxin-3 Recombinant Protein Lyophilized |
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Human Relaxin-3 Recombinant Protein Lyophilized |
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| MBS8433281-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Human Relaxin-2 |
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| MBS146516-0005mg | MyBiosource | 0.005mg | EUR 240 |
Recombinant Human Relaxin-2 |
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| MBS146516-0025mg | MyBiosource | 0.025mg | EUR 310 |
Recombinant Human Relaxin-2 |
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| MBS146516-1mg | MyBiosource | 1mg | EUR 2880 |
Design of a digital-PCR assay to quantify fragmented human mitochondrial DNA
Digital PCR (dPCR) has been tailored to quantify the proportion of mitochondrial DNA (mtDNA) molecules with out and with double-strand DNA breaks (DSBs). That is based mostly on a break-apart strategy of two differentially labeled goal sequences distantly situated within the round DNA molecule. When the 2 targets amplify in separated response partitions, the unique mtDNA molecule ought to be fragmented by two DSBs not less than, every in a unique phase between targets.
When each targets amplify in the identical partition, it should correspond to a round or linear mtDNA molecule. These two prospects could also be distinguished via a restriction endonuclease (RE) induced distinctive DSB inside a DNA phase between the targets. After RE-digestion, separation of each goal alerts in several partitions should point out the presence of a earlier linear mtDNA molecule. In any other case, joint amplification in the identical partition would correspond to an preliminary round mtDNA, that has been linearized by the endonuclease.
The process was validated by assaying totally different proportions of mtDNA fragmented by in vitro digestion with REs, evidencing an ideal accordance beween the anticipated theoretical values and dPCR quantification. Samples from peripheral blood cells, mobile and extracellular DNA from the U2OS cell line, in addition to cells incubated with ethidium bromide to induce mtDNA depletion, had been evaluated. The method could also be of curiosity to enhance the research of mtDNA in relation to growing older and human illness, in addition to to evaluate potential antagonistic results of sure medication that may very well be associated to affectation of mtDNA. This text is protected
Comparability of mitochondrial genome and improvement of particular PCR primers for figuring out two scuticociliates, Pseudocohnilembus persalinus and Uronema marinum
Background: Pseudocohnilembus persalinus and Uronema marinum (Ciliophora, Scuticociliatia), as parasitic scuticociliatid ciliates, had been remoted from Scophthalmus maximus and Takifugu rubripes, respectively, in our earlier research. These ciliates are morphologically very comparable; therefore, it’s troublesome to determine particular scuticociliate species utilizing conventional classification strategies for performing taxonomic analysis and illness management research.
Strategies: We annotated the mitochondrial genomes of those two scuticociliates on the premise of earlier sequencing, together with analyses of nucleotide composition, codon utilization, Ka/Ks, and p-distance. We additionally in contrast the nucleotide and amino acid similarity of the mitochondrial genomes of P. persalinus, U. marinum, and different 12 associated ciliates, and a phylogenetic tree was constructed utilizing 16 widespread genes. We selected the nad4 and nad7 genes to design particular PCR primers for identification.
Outcomes: P. persalinus and U. marinum had been discovered to have a detailed evolutionary relationship. Though codon preferences had been comparable, variations had been noticed within the utilization of codons reminiscent of CGA, CGC, and GTC. Each Ka/Ks and p-distance had been lower than 1. Apart from yejR, ymf57, ymf67, and ymf75, the amino acid sequence similarity between P. persalinus and U. marinum was higher than 50%.
Conclusions: The mitochondrial genomes of P. persalinus and U. marinum had been completely in contrast to offer a reference for illness prevention and management. The precise PCR primers enabled us to determine P. persalinus and U. marinum quickly and precisely on the molecular degree, thus offering a foundation for classification and identification.
Estimating Clinically Related Minimize-Off Values for a Excessive-Throughput Quantitative Actual-Time PCR Detecting Bacterial Respiratory Pathogens in Cattle
Bovine respiratory illness (BRD) outcomes from interactions between pathogens, environmental stressors, and host elements. Acquiring a analysis of the causal pathogens is difficult however using high-throughput real-time PCR (rtPCR) might assist goal preventive and therapeutic interventions.
The intention of this examine was to enhance the interpretation of rtPCR outcomes by analysing their associations with medical observations. The target was to develop and illustrate a field-data pushed statistical technique to information the collection of related quantification cycle cut-off values for pathogens related to BRD for the high-throughput rtPCR system “Fluidigm BioMark HD” based mostly on nasal swabs from calves. We used information from 36 herds enrolled in a Danish subject examine the place 340 calves inside pre-determined age-groups had been topic to medical examination and nasal swabs as much as 4 occasions.
The samples had been analysed with the rtPCR system. Every of the 1,025 statement models had been categorised as sick with BRD or wholesome, based mostly on medical scores. The optimum rtPCR outcomes to foretell BRD had been investigated for Pasteurella multocida, Mycoplasma bovis, Histophilus somni, Mannheimia haemolytica, and Trueperella pyogenes by decoding scatterplots and outcomes of blended results logistic regression fashions.
The clinically related rtPCR cut-off prompt for P. multocida and M. bovis was ≤ 21.3. For H. somni it was ≤ 17.4, whereas no cut-off may very well be decided for M. haemolytica and T. pyogenes. The demonstrated strategy can present goal help within the alternative of clinically related cut-offs. Nonetheless, for strong efficiency of the regression mannequin enough quantities of appropriate information are required.
HCV Real Time RT-PCR Kit |
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| GWB-LRB026 | GenWay Biotech | 25 tests | Ask for price |
HDV Real Time RT-PCR Kit |
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| GWB-LRB029 | GenWay Biotech | 25 tests | Ask for price |
HDV Real Time RT-PCR Kit |
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| GWB-LRB030 | GenWay Biotech | 25 tests | Ask for price |
HCMV Real Time PCR Kit |
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| GWB-LRB009 | GenWay Biotech | 25 tests | Ask for price |
HCMV Real Time PCR Kit |
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| GWB-LRB010 | GenWay Biotech | 25 tests | Ask for price |
HEV IV Real Time RT-PCR Kit |
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| GWB-LRB031 | GenWay Biotech | 25 tests | Ask for price |
HEV IV Real Time RT-PCR Kit |
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| GWB-LRB032 | GenWay Biotech | 25 tests | Ask for price |
ONE STEP RT-PCR REAL TIME KIT |
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| MBT128-10R | EWC Diagnostics | 1 unit | EUR 37.4 |
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Description: ONE STEP RT-PCR REAL TIME KIT |
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ONE STEP RT-PCR REAL TIME KIT |
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| MBT128-25R | EWC Diagnostics | 1 unit | EUR 89.72 |
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Description: ONE STEP RT-PCR REAL TIME KIT |
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ONE STEP RT-PCR REAL TIME KIT |
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| MBT128-2X50R | EWC Diagnostics | 1 unit | EUR 314.51 |
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Description: ONE STEP RT-PCR REAL TIME KIT |
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ONE STEP RT-PCR REAL TIME KIT |
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| MBT128-50R | EWC Diagnostics | 1 unit | EUR 165.56 |
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Description: ONE STEP RT-PCR REAL TIME KIT |
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HCV Genetype Real Time RT-PCR Kit |
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| GWB-LRB027 | GenWay Biotech | 25 tests | Ask for price |
HCV Genetype Real Time RT-PCR Kit |
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| GWB-LRB028 | GenWay Biotech | 25 tests | Ask for price |
RV Real Time RT-PCR Detection kit |
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| TRI-L02M1 | TRI Biotech | 32T | EUR 705.6 |
RV Real Time RT-PCR Detection kit |
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| TRI-L02S1 | TRI Biotech | 16T | EUR 378 |
HLA B27 Real-TM Real Time PCR kit |
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| R116-50FRT | Sacace Biotechnologies | 48 | EUR 627.84 |
Measles Virus Real Time RT-PCR Kit |
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| GWB-LRB043 | GenWay Biotech | 25 tests | Ask for price |
Measles Virus Real Time RT-PCR Kit |
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| GWB-LRB044 | GenWay Biotech | 25 tests | Ask for price |
Rubella Virus Real Time RT-PCR Kit |
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| GWB-LRB045 | GenWay Biotech | 25 tests | Ask for price |
Rubella Virus Real Time RT-PCR Kit |
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| GWB-LRB046 | GenWay Biotech | 25 tests | Ask for price |
FCV Real Time RT-PCR Detection kit |
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| TRI-L14M1 | TRI Biotech | 32T | EUR 705.6 |
FCV Real Time RT-PCR Detection kit |
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| TRI-L14S1 | TRI Biotech | 16T | EUR 378 |
FIV Real Time RT-PCR Detection kit |
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| TRI-L22M1 | TRI Biotech | 32T | EUR 705.6 |
FIV Real Time RT-PCR Detection kit |
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| TRI-L22S1 | TRI Biotech | 16T | EUR 378 |
FIPV Real Time RT-PCR Detection kit |
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| TRI-L03M1 | TRI Biotech | 32T | EUR 453.6 |
FIPV Real Time RT-PCR Detection kit |
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| TRI-L03S1 | TRI Biotech | 16T | EUR 252 |
CPIV Real Time RT-PCR Detection kit |
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| TRI-L07M1 | TRI Biotech | 32T | EUR 453.6 |
CPIV Real Time RT-PCR Detection kit |
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| TRI-L07S1 | TRI Biotech | 16T | EUR 252 |
CSFV Real Time RT-PCR Detection kit |
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| TRI-L28M1 | TRI Biotech | 48T | EUR 1008 |
CSFV Real Time RT-PCR Detection kit |
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| TRI-L28S1 | TRI Biotech | 24T | EUR 529.2 |
PEDV Real Time RT-PCR Detection kit |
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| TRI-L34M1 | TRI Biotech | 48T | EUR 1008 |
PEDV Real Time RT-PCR Detection kit |
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| TRI-L34S1 | TRI Biotech | 24T | EUR 529.2 |
CP&MP Real Time PCR Kit |
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| GWB-LRB005 | GenWay Biotech | 25 tests | Ask for price |
CP&MP Real Time PCR Kit |
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| GWB-LRB006 | GenWay Biotech | 25 tests | Ask for price |
MRSA Quant Real-TM Real Time PCR kit |
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| B78-100FRT | Sacace Biotechnologies | 100 | EUR 784.8 |
Chikungunya Virus Real Time RT-PCR Kit |
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| GWB-LRB001 | GenWay Biotech | 25 tests | Ask for price |
Chikungunya Virus Real Time RT-PCR Kit |
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| GWB-LRB002 | GenWay Biotech | 25 tests | Ask for price |
Yersinia pestis Real-TM Real Time PCR kit |
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| B79-50FRT | Sacace Biotechnologies | 50 | EUR 627.84 |
Monkeypox Virus Real Time PCR Kit |
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| YJC70115NW-25T | Jiangsu Bioperfectus Technologies | 25 tests/kit | Ask for price |
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Description: The Bioperfectus Monkeypox Virus Real Time PCR Kit is an in vitro diagnostic test, based on real-time PCR technology, for the detection of DNA from the Monkeypox virus. Specimens can be obtained from human serum, lesion exudate samples and scab. BSL-2 facilities with standard BSL-2 work practices may be used for the test of t he Monkeypox virus. |
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Monkeypox Virus Real Time PCR Kit |
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| ZD-0076-01 | Gentaur Genprice | 25 tests/kit | Ask for price |
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Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load. |
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Monkeypox Virus Real Time PCR Kit |
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| ZD-0076-02 | Gentaur Genprice | 25 tests/kit | Ask for price |
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Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load. |
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Monkeypox Virus Real Time PCR Kit |
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| PDPS-AR064 | Creative Biogene | 1 unit | Ask for price |
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Description: Creative Biogene Monkeypox Virus Real Time PCR Kit is used for the detection of monkeypox Virus in serum or lesion exudate samples by using real time PCR systems. Monkeypox virus (MPV) is a double-stranded DNA, zoonotic virus and a species of the genus Orthopoxvirus in the family Poxviridae. It is one of the human orthopoxviruses that includes variola (VARV), cowpox (CPX), and vaccinia (VACV) viruses. The kit contains a specific ready-to-use system for the detection of the monkeypox Virus. Fluorescence is emitted and measured by the real time systems' optical unit during the PCR. |
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VetAlert Johnes Real Time PCR Kit |
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| TC-9828-100 | Tetracore | 100 rxns | EUR 460 |
Staphilococcus aureus Real-TM Real Time PCR kit |
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| B1772-96FRT | Sacace Biotechnologies | 96 | EUR 562.44 |
HBV Quantitative Real Time PCR Kit |
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| GWB-LRB013 | GenWay Biotech | 25 tests | Ask for price |
HBV Quantitative Real Time PCR Kit |
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| GWB-LRB014 | GenWay Biotech | 25 tests | Ask for price |
Toxoplasma Gondii Real Time PCR Kit |
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| GWB-LRB049 | GenWay Biotech | 25 tests | Ask for price |
Toxoplasma Gondii Real Time PCR Kit |
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| GWB-LRB050 | GenWay Biotech | 25 tests | Ask for price |
HEV I & III Real Time RT-PCR Kit |
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| GWB-LRB033 | GenWay Biotech | 25 tests | Ask for price |
HEV I & III Real Time RT-PCR Kit |
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| GWB-LRB034 | GenWay Biotech | 25 tests | Ask for price |
CHV Real Time PCR Detection kit |
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| TRI-L09M1 | TRI Biotech | 32T | EUR 453.6 |
CHV Real Time PCR Detection kit |
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| TRI-L09S1 | TRI Biotech | 16T | EUR 252 |
PCVⅡ Real Time PCR Detection kit |
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| TRI-L29M1 | TRI Biotech | 48T | EUR 705.6 |
PCVⅡ Real Time PCR Detection kit |
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| TRI-L29S1 | TRI Biotech | 24T | EUR 378 |
ASFV Real Time PCR Detection kit |
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| TRI-L04M1C | TRI Biotech | 48T | EUR 554.4 |
ASFV Real Time PCR Detection kit |
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| TRI-L04S1C | TRI Biotech | 24T | EUR 302.4 |
HEV General Type Real Time RT-PCR Kit |
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| GWB-LRB037 | GenWay Biotech | 25 tests | Ask for price |
HEV General Type Real Time RT-PCR Kit |
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| GWB-LRB038 | GenWay Biotech | 25 tests | Ask for price |
BioRT Real Time RT-PCR Kit（SYBR Green） |
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| TRI-B33M1 | TRI Biotech | 100T | EUR 1058.4 |
BioRT Real Time RT-PCR Kit（SYBR Green） |
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| TRI-B33S1 | TRI Biotech | 50T | EUR 554.4 |
Babesia Real Time PCR Detection kit |
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| TRI-L20M1 | TRI Biotech | 32T | EUR 453.6 |
Babesia Real Time PCR Detection kit |
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| TRI-L20S1 | TRI Biotech | 16T | EUR 252 |
Giardia Real Time PCR Detection kit |
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| TRI-L26M1 | TRI Biotech | 32T | EUR 453.6 |
Giardia Real Time PCR Detection kit |
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| TRI-L26S1 | TRI Biotech | 16T | EUR 252 |
Oncogenetics BRCA Panel Real-TM Real Time PCR kit |
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| R-27-P-48FRT | Sacace Biotechnologies | 48 | EUR 1907.5 |
Brucella Real Time PCR Detection kit |
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| TRI-L24M1 | TRI Biotech | 32T | EUR 453.6 |
Brucella Real Time PCR Detection kit |
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| TRI-L24S1 | TRI Biotech | 16T | EUR 252 |
Chlamydia Real Time PCR Detection kit |
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| TRI-L15M1 | TRI Biotech | 32T | EUR 453.6 |
Chlamydia Real Time PCR Detection kit |
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| TRI-L15S1 | TRI Biotech | 16T | EUR 252 |
Human Lung Cancer PCR Primer Library |
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| HLUCPL-I | Real Time Primers | 1 set | EUR 657.6 |
Human Schwann Cell PCR Primer Library |
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| HSCH-I | Real Time Primers | 1 set | EUR 657.6 |
Leptospira Real Time PCR Detection kit |
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| TRI-L13M1 | TRI Biotech | 32T | EUR 453.6 |
Leptospira Real Time PCR Detection kit |
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| TRI-L13S1 | TRI Biotech | 16T | EUR 252 |
Mycoplasma Real Time PCR Detection kit |
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| TRI-L16M1 | TRI Biotech | 32T | EUR 453.6 |
Mycoplasma Real Time PCR Detection kit |
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| TRI-L16S1 | TRI Biotech | 16T | EUR 252 |
Ehrilichia Real Time PCR Detection kit |
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| TRI-L25M1 | TRI Biotech | 32T | EUR 453.6 |
Ehrilichia Real Time PCR Detection kit |
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| TRI-L25S1 | TRI Biotech | 16T | EUR 252 |
Edwardsiella Real Time PCR Detection kit |
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| TRI-N14M1 | TRI Biotech | 32T | EUR 491.4 |
Edwardsiella Real Time PCR Detection kit |
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| TRI-N14S1 | TRI Biotech | 16T | EUR 289.8 |
PRRSV Universal Real Time RT-PCR Detection kit |
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| TRI-L33M1 | TRI Biotech | 48T | EUR 1008 |
PRRSV Universal Real Time RT-PCR Detection kit |
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| TRI-L33S1 | TRI Biotech | 24T | EUR 529.2 |
HCV/HBV/HIV1/HIV2 Real-TM Real Time PCR kit |
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| V62-100FRT | Sacace Biotechnologies | 100 | EUR 1560.88 |
Chlamydia Pheumoniae (CP) Real Time PCR Kit |
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| GWB-LRB003 | GenWay Biotech | 25 tests | Ask for price |
Chlamydia Pheumoniae (CP) Real Time PCR Kit |
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| GWB-LRB004 | GenWay Biotech | 25 tests | Ask for price |
PRV (gE) Real Time PCR Detection kit |
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| TRI-L30M1 | TRI Biotech | 48T | EUR 705.6 |
PRV (gE) Real Time PCR Detection kit |
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| TRI-L30S1 | TRI Biotech | 24T | EUR 378 |
COMT Val158Met SNP-Screen Real-TM Real Time PCR kit |
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| T01352-50-T | Sacace Biotechnologies | 60 | EUR 538.46 |
FHV-1 Real Time PCR Detection kit |
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| TRI-L01M1 | TRI Biotech | 32T | EUR 453.6 |
FHV-1 Real Time PCR Detection kit |
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| TRI-L01S1 | TRI Biotech | 16T | EUR 252 |
BP/MP/CP Multiplex Real Time PCR Kit |
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| GWB-LRB007 | GenWay Biotech | 25 tests | Ask for price |
BP/MP/CP Multiplex Real Time PCR Kit |
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| GWB-LRB008 | GenWay Biotech | 25 tests | Ask for price |
Fcov and Ccov Real Time RT-PCR Detection kit |
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| TRI-L11M1 | TRI Biotech | 32T | EUR 705.6 |
Fcov and Ccov Real Time RT-PCR Detection kit |
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| TRI-L11S1 | TRI Biotech | 16T | EUR 378 |
Vibrio Harveyi Real Time PCR Detection Kit |
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| TRI-N04M1 | TRI Biotech | 32T | EUR 453.6 |
Vibrio Harveyi Real Time PCR Detection Kit |
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| TRI-N04S1 | TRI Biotech | 16T | EUR 252 |
Babesia gibsoni Real Time PCR Detection kit |
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| TRI-L21M1 | TRI Biotech | 32T | EUR 453.6 |
Babesia gibsoni Real Time PCR Detection kit |
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| TRI-L21S1 | TRI Biotech | 16T | EUR 252 |
Bacteria/Vibrio Real Time PCR Detection Kit |
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| TRI-N06M1 | TRI Biotech | 32T | EUR 529.2 |
Bacteria/Vibrio Real Time PCR Detection Kit |
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| TRI-N06S1 | TRI Biotech | 16T | EUR 289.8 |
Koi Herpesvirus Real Time PCR Detection kit |
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| TRI-N18M1 | TRI Biotech | 32T | EUR 491.4 |
Koi Herpesvirus Real Time PCR Detection kit |
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| TRI-N18S1 | TRI Biotech | 16T | EUR 289.8 |
HCV/HBV/HIV/HIV2 Real-TM Real Time PCR kit (lyophilized) |
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| V50-50FRT-L | Sacace Biotechnologies | 50 | EUR 937.4 |
Toxoplasma gondii Real Time PCR Detection kit |
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| TRI-L19M1 | TRI Biotech | 32T | EUR 453.6 |
Toxoplasma gondii Real Time PCR Detection kit |
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| TRI-L19S1 | TRI Biotech | 16T | EUR 252 |
Baculovirus Penaei Real Time PCR Detection kit |
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| TRI-N12M1 | TRI Biotech | 32T | EUR 491.4 |
Baculovirus Penaei Real Time PCR Detection kit |
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| TRI-N12S1 | TRI Biotech | 16T | EUR 289.8 |
qTOWER3 auto Real Time PCR system |
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| AJ844-00603-2 | Westburg | each | EUR 55045 |
Canine Distemper virus Real Time RT-PCR Detection kit |
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| TRI-L10M1 | TRI Biotech | 32T | EUR 705.6 |
Canine Distemper virus Real Time RT-PCR Detection kit |
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| TRI-L10S1 | TRI Biotech | 16T | EUR 378 |
HEV I, II, III, & IV Real Time RT-PCR Kit |
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| GWB-LRB035 | GenWay Biotech | 25 tests | Ask for price |
HEV I, II, III, & IV Real Time RT-PCR Kit |
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| GWB-LRB036 | GenWay Biotech | 25 tests | Ask for price |
HBV Quantitative & YMDD Mutation Real Time PCR Kit |
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| GWB-LRB015 | GenWay Biotech | 25 tests | Ask for price |
HBV Quantitative & YMDD Mutation Real Time PCR Kit |
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| GWB-LRB016 | GenWay Biotech | 25 tests | Ask for price |
HBV Quantitative & YMDD Mutation Real Time PCR Kit |
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| GWB-LRB017 | GenWay Biotech | 25 tests | Ask for price |
HBV Quantitative & YMDD Mutation Real Time PCR Kit |
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| GWB-LRB018 | GenWay Biotech | 25 tests | Ask for price |
Mycoplasma haemofelis Real Time PCR Detection kit |
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| TRI-L17M1 | TRI Biotech | 32T | EUR 453.6 |
Mycoplasma haemofelis Real Time PCR Detection kit |
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| TRI-L17S1 | TRI Biotech | 16T | EUR 252 |