Current Classification of the Bacillus pumilus Group Species

Present Classification of the Bacillus pumilus Group Species, the Rubber-Pathogenic Micro organism Inflicting Trunk Bulges Illness in Malaysia as Assessed by MLSA and Multi rep-PCR Approaches

Bacillus pumilus is the causal agent of trunk bulges illness affecting rubber and rubberwood high quality and yield manufacturing. On this examine, B. pumilus and different carefully associated species had been included in B. pumilus group, as they shared over 99.5% similarity from 16S rRNA evaluation. Multilocus sequence evaluation (MLSA) of 5 housekeeping genes and repetitive elements-based polymerase chain response (rep-PCR) utilizing REP, ERIC, and BOX primers carried out to investigate the variety and systematic relationships of 20 isolates of B. pumilus group from 4 rubber tree plantations in Peninsular Malaysia (Serdang, Tanah Merah, Baling, and Rawang).
Multi rep-PCR outcomes revealed the genetic profiling among the many B. pumilus group isolates, whereas MLSA outcomes confirmed 98-100% similarity throughout the 20 isolates of B. pumilus group species. These 20 isolates, previously established as B. pumilus, had been discovered to not be grouped with B. pumilus. Nonetheless, being distributed inside distinctive teams of the B. pumilus group comprising of two clusters, A and B. Cluster A contained of 17 isolates near B. altitudinis, whereas Cluster B consisted of three isolates attributed to B. safensis. That is the primary MLSA and rep-PCR examine on B. pumilus group, which offers an in-depth understanding of the variety of those rubber-pathogenic isolates in Malaysia.
russelldurrett
russelldurrett

Anti-Relaxin 1 (1H6)

YF-MA15197 100 ug
EUR 363
Description: Mouse monoclonal to Relaxin 1

Recombinant Human Relaxin-3 Protein

PROTQ8WXF3-1 25ug
EUR 317
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as “somatostatin-like” or “angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an “orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain.

Anti-VEGF Receptor 3/FLT4 Antibody

A01276-3 100ug/vial
EUR 334

Anti-Relaxin 1/RLN1 Antibody

A08367-1 100ug/vial
EUR 334

Anti-Relaxin Receptor 2 Antibody

A04848 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Relaxin Receptor 2 Antibody (RXFP2) detection.tested for WB in Human, Mouse, Rat.

Anti-Relaxin Receptor 2 antibody

STJ95407 200 µl
EUR 197
Description: Rabbit polyclonal to Relaxin Receptor 2.

Anti-Relaxin Receptor 4 antibody

STJ95409 200 µl
EUR 197
Description: Rabbit polyclonal to Relaxin Receptor 4.

Anti-Relaxin 1 Antibody BIOTIN

STJ502817 100 µg
EUR 586

Anti-Relaxin 1 Antibody FITC

STJ502818 100 µg
EUR 586

Anti-14-3-3 alpha + beta Rabbit Monoclonal Antibody

M02431-3 100ug/vial
EUR 397
Description: Rabbit Monoclonal 14-3-3 alpha + beta Antibody. Validated in Flow Cytometry, IP, IF, IHC, ICC, WB and tested in Human, Mouse, Rat.

Relaxin 3 Polyclonal Antibody

ES4671-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Relaxin 3 from Human. This antibody is tested and validated for IHC, WB, ELISA

Relaxin 3 Polyclonal Antibody

ES4671-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Relaxin 3 from Human. This antibody is tested and validated for IHC, WB, ELISA

Relaxin 3 (RLN3) Antibody

20-abx130221
  • EUR 453.00
  • EUR 133.00
  • EUR 1302.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Relaxin 3 (RLN3) Antibody

20-abx121705
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Relaxin 3 Polyclonal Antibody

ABP53672-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of Relaxin 3 from Human. This Relaxin 3 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin 3

Relaxin 3 Polyclonal Antibody

ABP53672-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of Relaxin 3 from Human. This Relaxin 3 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin 3

Relaxin 3 Polyclonal Antibody

ABP53672-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of Relaxin 3 from Human. This Relaxin 3 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin 3

Relaxin 3 (RLN3) Antibody

20-abx323035
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

Relaxin 3 (RLN3) Antibody

20-abx218313
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug

Relaxin 3 Blocking Peptide

20-abx161415
  • EUR 606.00
  • EUR 1428.00
  • 1 mg
  • 5 mg

Relaxin 3 (RLN3) Antibody

20-abx178243
  • EUR 1233.00
  • EUR 592.00
  • 1 mg
  • 200 ug

Relaxin 3 (RLN3) Antibody

20-abx178244
  • EUR 1233.00
  • EUR 592.00
  • 1 mg
  • 200 ug

Relaxin 3 (RLN3) Antibody

20-abx174368
  • EUR 356.00
  • EUR 913.00
  • EUR 467.00
  • EUR 154.00
  • EUR 272.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Recombinant Relaxin 3 (RLN3)

4-RPD869Ra01
  • EUR 490.66
  • EUR 234.00
  • EUR 1564.96
  • EUR 588.32
  • EUR 1076.64
  • EUR 391.00
  • EUR 3762.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Relaxin 3 expressed in: E.coli

Relaxin-3 Recombinant Protein

40-230-0005mg 0.005 mg
EUR 259.25
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as "somatostatin-like” or "angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an "orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain.

Relaxin-3 Recombinant Protein

40-230-0025mg 0.025 mg
EUR 364.25
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as "somatostatin-like” or "angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an "orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain.

Relaxin H2 (human)

H-6784.0200 0.2mg
EUR 618
Description: Sum Formula: C256H408N74O74S8; CAS# [99489-94-8] net

Relaxin H2 (human)

H-6784.1000 1.0mg
EUR 2378
Description: Sum Formula: C256H408N74O74S8; CAS# [99489-94-8] net

Relaxin H3 (human)

H-7322.0200 0.2mg
EUR 618
Description: Sum Formula: C237H374N70O69S6; CAS# [1158181-62-4] net

Relaxin H3 (human)

H-7322.1000 1.0mg
EUR 2378
Description: Sum Formula: C237H374N70O69S6; CAS# [1158181-62-4] net

Anti-Human Ki67 Antibody

M00254-3 100ul
EUR 397
Description: Rabbit Polyclonal Human Ki67 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

Anti-Human IBA1 Antibody

M01394-3 100ul
EUR 397
Description: Chicken Polyclonal Human IBA1 Antibody. Validated in IF, IHC, WB and tested in Human.

Anti-active Caspase-3 Rabbit Monoclonal Antibody

M00334-3 100ug/vial
EUR 397
Description: Anti-active Caspase-3 Rabbit Monoclonal Antibody tested for IF, IHC, ICC, WB in Human

Anti-Galectin 3/LGALS3 Antibody (monoclonal, 12B12)

M00621-3 100ug/vial
EUR 334

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Human Relaxin 3 (RLN3) ELISA Kit

abx512123-96tests 96 tests
EUR 754

Human RLN3/ Relaxin-3 ELISA Kit

E2158Hu 1 Kit
EUR 571

Human Relaxin 3 (RLN3) ELISA Kit

abx253713-96tests 96 tests
EUR 754

RLN3 Relaxin-3 Human Recombinant Protein

PROTQ8WXF3 Regular: 25ug
EUR 317
Description: Relaxin-3 Human Recombinant produced in E.Coli is a disulfide-linked heterodimeric, non-glycosylated, polypeptide chain containing 24 amino acids for A chain and 27 amino acids for B chain and having a molecular mass of 2.5kDa for A chain and 3kDa for B chain.;The Relaxin-3 is purified by proprietary chromatographic techniques.

ELISA kit for Human Relaxin 3

EK5653 96 tests
EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Relaxin 3 in samples from serum, plasma, tissue homogenates and other biological fluids.

Human Relaxin 3(RLN3)ELISA Kit

QY-E01376 96T
EUR 361

Human RLN3(Relaxin-3) ELISA Kit

EH0755 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Anti-Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Monoclonal Antibody

M00227-3 100ul
EUR 397
Description: Mouse Monoclonal Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Antibody. Validated in IHC, WB and tested in Equine.

Relaxin Receptor 3 Polyclonal Antibody

ES6513-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Relaxin Receptor 3 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Relaxin Receptor 3 Polyclonal Antibody

ES6513-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Relaxin Receptor 3 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Relaxin Receptor 3 Polyclonal Antibody

ABP55514-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of Relaxin Receptor 3 from Human, Mouse, Rat. This Relaxin Receptor 3 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin Receptor 3 at AA range: 130-210

Relaxin Receptor 3 Polyclonal Antibody

ABP55514-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of Relaxin Receptor 3 from Human, Mouse, Rat. This Relaxin Receptor 3 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin Receptor 3 at AA range: 130-210

Relaxin Receptor 3 Polyclonal Antibody

ABP55514-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of Relaxin Receptor 3 from Human, Mouse, Rat. This Relaxin Receptor 3 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin Receptor 3 at AA range: 130-210

Relaxin 3 receptor 1 Antibody

abx019161-100ug 100 ug
EUR 342

Rat Relaxin 3 (RLN3) Protein

20-abx168490
  • EUR 690.00
  • EUR 286.00
  • EUR 2110.00
  • EUR 815.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Relaxin 3 (RLN3) Protein

20-abx654935
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Relaxin 3 (RLN3) Protein

20-abx654936
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Relaxin 3 receptor 1 Antibody

48026-100ul 100ul
EUR 333

Relaxin 3 receptor 1 Antibody

48026-50ul 50ul
EUR 239

ELISA kit for Human RLN3 (Relaxin-3)

E-EL-H1652 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Human RLN3 (Relaxin-3) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human Relaxin-3 (RLN3)

KTE60790-48T 48T
EUR 332
Description: Quantitative sandwich ELISA for measuring Human Relaxin-3 (RLN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Relaxin-3 (RLN3)

KTE60790-5platesof96wells 5 plates of 96 wells
EUR 2115
Description: Quantitative sandwich ELISA for measuring Human Relaxin-3 (RLN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Relaxin-3 (RLN3)

KTE60790-96T 96T
EUR 539
Description: Quantitative sandwich ELISA for measuring Human Relaxin-3 (RLN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Human Relaxin (RLN) Protein

20-abx654931
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Relaxin ELISA Kit

ELA-E1216h 96 Tests
EUR 824

Relaxin-2, human recombinant

7194-10
EUR 207

Relaxin-2, human recombinant

7194-50
EUR 675

Human Relaxin ELISA kit

E01R0008-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Human Relaxin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Relaxin ELISA kit

E01R0008-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Human Relaxin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Relaxin ELISA kit

E01R0008-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Human Relaxin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Anti-MRP1 (human) Monoclonal Antibody (QCRL-1)

M00872-3 1ml
EUR 675
Description: Mouse Monoclonal MRP1 (human) Antibody (QCRL-1). Validated in IP, IF and tested in Human.

Porcine relaxin peptides / relaxin (RLN) ELISA Kit

QY-E40036 96T
EUR 400

Anti-Human IgM Rabbit Monoclonal Antibody, Clone#RM121

M07469-3 100ug
EUR 375
Description: Anti-Human IgM Rabbit Monoclonal Antibody, Clone#RM121 tested in ICC, IHC, FC, ELISA, reactive to Human

Anti-CNP Antibody

M01017-3 100ul
EUR 397
Description: Chicken Polyclonal CNP Antibody. Validated in IHC, WB and tested in Equine, Pig.

Anti-GAP43 Antibody

M01868-3 100ul
EUR 397
Description: Rabbit Polyclonal GAP43 Antibody. Validated in IF, IHC, WB and tested in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat.

Anti-Calretinin Antibody

M04255-3 100ul
EUR 397
Description: Rabbit Polyclonal Calretinin Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-Nanog Antibody

A00153-3 100ug/vial
EUR 294

Anti-IL17C Antibody

A00164-3 100ug/vial
EUR 294

Anti-AICDA Antibody

A00267-3 100ug/vial
EUR 294

Anti-ICOS Antibody

A00291-3 100ug/vial
EUR 334

Anti-TLR1 Antibody

A00429-3 100ug/vial
EUR 334

Anti-IGFBP3 Antibody

A00435-3 100ug/vial
EUR 294

Anti-IL17B Antibody

A10846-3 100ug/vial
EUR 294

Anti-RAB3GAP2 Antibody

A07244-3 100ug/vial
EUR 334

Anti-FOXI1 Antibody

A07512-3 100ug/vial
EUR 334

Anti-TANK Antibody

A00445-3 100ug/vial
EUR 334

Anti-FGF23 Antibody

A00478-3 100ug/vial
EUR 294

Anti-Leptin Antibody

A00479-3 100ug/vial
EUR 294

Anti-CD5 Antibody

A00480-3 100ug/vial
EUR 334

Anti-SYK Antibody

A00490-3 100ug/vial
EUR 294

Anti-PON1 Antibody

A00516-3 100ug/vial
EUR 334

Anti-CSF1 Antibody

A00620-3 100ug/vial
EUR 294

Anti-LBP Antibody

A00809-3 100ug/vial
EUR 294

Anti-IL22 Antibody

A00963-3 100ug/vial
EUR 294

Design of a digital-PCR assay to quantify fragmented human mitochondrial DNA

Digital PCR (dPCR) has been tailored to quantify the proportion of mitochondrial DNA (mtDNA) molecules with out and with double-strand DNA breaks (DSBs). That is based mostly on a break-apart strategy of two differentially labeled goal sequences distantly situated within the round DNA molecule. When the 2 targets amplify in separated response partitions, the unique mtDNA molecule ought to be fragmented by two DSBs not less than, every in a unique phase between targets.
When each targets amplify in the identical partition, it should correspond to a round or linear mtDNA molecule. These two prospects could also be distinguished via a restriction endonuclease (RE) induced distinctive DSB inside a DNA phase between the targets. After RE-digestion, separation of each goal alerts in several partitions should point out the presence of a earlier linear mtDNA molecule. In any other case, joint amplification in the identical partition would correspond to an preliminary round mtDNA, that has been linearized by the endonuclease.
The process was validated by assaying totally different proportions of mtDNA fragmented by in vitro digestion with REs, evidencing an ideal accordance beween the anticipated theoretical values and dPCR quantification. Samples from peripheral blood cells, mobile and extracellular DNA from the U2OS cell line, in addition to cells incubated with ethidium bromide to induce mtDNA depletion, had been evaluated. The method could also be of curiosity to enhance the research of mtDNA in relation to growing older and human illness, in addition to to evaluate potential antagonistic results of sure medication that may very well be associated to affectation of mtDNA. This text is protected

Comparability of mitochondrial genome and improvement of particular PCR primers for figuring out two scuticociliates, Pseudocohnilembus persalinus and Uronema marinum

Background: Pseudocohnilembus persalinus and Uronema marinum (Ciliophora, Scuticociliatia), as parasitic scuticociliatid ciliates, had been remoted from Scophthalmus maximus and Takifugu rubripes, respectively, in our earlier research. These ciliates are morphologically very comparable; therefore, it’s troublesome to determine particular scuticociliate species utilizing conventional classification strategies for performing taxonomic analysis and illness management research.
Strategies: We annotated the mitochondrial genomes of those two scuticociliates on the premise of earlier sequencing, together with analyses of nucleotide composition, codon utilization, Ka/Ks, and p-distance. We additionally in contrast the nucleotide and amino acid similarity of the mitochondrial genomes of P. persalinus, U. marinum, and different 12 associated ciliates, and a phylogenetic tree was constructed utilizing 16 widespread genes. We selected the nad4 and nad7 genes to design particular PCR primers for identification.
Outcomes: P. persalinus and U. marinum had been discovered to have a detailed evolutionary relationship. Though codon preferences had been comparable, variations had been noticed within the utilization of codons reminiscent of CGA, CGC, and GTC. Each Ka/Ks and p-distance had been lower than 1. Apart from yejR, ymf57, ymf67, and ymf75, the amino acid sequence similarity between P. persalinus and U. marinum was higher than 50%.
Conclusions: The mitochondrial genomes of P. persalinus and U. marinum had been completely in contrast to offer a reference for illness prevention and management. The precise PCR primers enabled us to determine P. persalinus and U. marinum quickly and precisely on the molecular degree, thus offering a foundation for classification and identification.

Estimating Clinically Related Minimize-Off Values for a Excessive-Throughput Quantitative Actual-Time PCR Detecting Bacterial Respiratory Pathogens in Cattle

Bovine respiratory illness (BRD) outcomes from interactions between pathogens, environmental stressors, and host elements. Acquiring a analysis of the causal pathogens is difficult however using high-throughput real-time PCR (rtPCR) might assist goal preventive and therapeutic interventions.
The intention of this examine was to enhance the interpretation of rtPCR outcomes by analysing their associations with medical observations. The target was to develop and illustrate a field-data pushed statistical technique to information the collection of related quantification cycle cut-off values for pathogens related to BRD for the high-throughput rtPCR system “Fluidigm BioMark HD” based mostly on nasal swabs from calves. We used information from 36 herds enrolled in a Danish subject examine the place 340 calves inside pre-determined age-groups had been topic to medical examination and nasal swabs as much as 4 occasions.
The samples had been analysed with the rtPCR system. Every of the 1,025 statement models had been categorised as sick with BRD or wholesome, based mostly on medical scores. The optimum rtPCR outcomes to foretell BRD had been investigated for Pasteurella multocida, Mycoplasma bovis, Histophilus somni, Mannheimia haemolytica, and Trueperella pyogenes by decoding scatterplots and outcomes of blended results logistic regression fashions.
The clinically related rtPCR cut-off prompt for P. multocida and M. bovis was ≤ 21.3. For H. somni it was ≤ 17.4, whereas no cut-off may very well be decided for M. haemolytica and T. pyogenes. The demonstrated strategy can present goal help within the alternative of clinically related cut-offs. Nonetheless, for strong efficiency of the regression mannequin enough quantities of appropriate information are required.

dsGreen for Real-Time PCR, 100×, 100 uL

11010 100 uL
EUR 59

dsGreen for Real-Time PCR, 100×, 1 mL

41010 1 mL
EUR 249

dsGreen for Real-Time PCR, 100×, 1.5 mL

51010 1.5 mL
EUR 331

dsGreen for Real-Time PCR, 100×, 2 mL

61010 2 mL
EUR 363

dsGreen for Real-Time PCR, 100×, 10 mL

81010 10 mL
EUR 869

dsGreen for Real-Time PCR, 100×, 50 mL

91010 50 mL
EUR 3169

dsGreen for Real-Time PCR, 100×, 5 mL

71010 5 mL
EUR 625

hsa-let-7a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097603
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097604
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097605
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097606
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097607
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097608
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7g Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097609
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7i Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097610
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097611
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-1 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097612
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-7 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097613
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-9 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097614
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-9* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097615
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-10a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097616
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-10b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097617
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-10a* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097618
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-15a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097619
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-15b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097620
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-16 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097622
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-18a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097625
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-18b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097626
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-19a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097627
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-19b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097628
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-20a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097629
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-20b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097630
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-21 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097631
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-22 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097632
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-23a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097633
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-23b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097634
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-25 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097636
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-26a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097637
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-26b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097638
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-27a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097639
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-27b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097640
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-29a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097642
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-29b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097643
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-29c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097644
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-30a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097645
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-30b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097647
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-30c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097648
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-30d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097650
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-30e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097651
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-31 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097652
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-32 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097653
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-33a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097654
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-34a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097657
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-34b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097659
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-92a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097661
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-92b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097662
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-93 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097663
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-95 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097664
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-96 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097665
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-98 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097666
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-99a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097667
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-99b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097669
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-100 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097671
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-101 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097673
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-103 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097675
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-105 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097676
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-106a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097677
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-106b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097678
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-107 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097679
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-124 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097680
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-125a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097681
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-127 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097686
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-129 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097689
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-130a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097690
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-130b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097691
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-132 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097692
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-133a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097693
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-133b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097694
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-134 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097695
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-135b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097696
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-137 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097699
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-138 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097700
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-139 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097701
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-140 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097702
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-141 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097703
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-143 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097706
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-145 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097708
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-146a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097709
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-149 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097711
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-150 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097712
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-151 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097714
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

hsa-mir-152 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097715
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

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